Restriction map for
pFN21AB7883
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AhdI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbrPI BbvCI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NdeI NotI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfuI SmlI SnaBI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1249 GACGTC GACGT^C
AccI 781 GTAGAC GT^[AC][GT]AC
AflIII 1667 ACATGT A^C[GA][CT]GT
AgeI 946 ACCGGT A^CCGGT
AocI 101 CCTTAGG CC^T[ACGT]AGG
Aor51HI 1537 AGCGCT AGC^GCT
Asp700I 189 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 852 GGATCC G^GATCC
BbeI 294 GGCGCC GGCGC^C
BbsI 1135 GTCTTC GAAGAC(2/6)
BcgI 1458 CGATGGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1135 GTCTTC GAAGAC(2/6)
BsaAI 1185 CACGTA [CT]AC^GT[GA]
BsaI 1579 GAGACC GGTCTC(1/5)
BsaWI 946 ACCGGT [AT]^CCGG[AT]
BsiEI 443 CGGCCG CG[GA][CT]^CG
BspLU11I 1667 ACATGT A^CATGT
BsrDI 1175 CATTGC GCAATG(2/0)
BssSI 592 CTCGTG CACGAG(-5/-1)
BstEII 276 GGTCACC G^GT[ACGT]ACC
BstPI 276 GGTCACC G^GT[ACGT]ACC
BstXI 1649 CCAGCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 101 CCTTAGG CC^T[ACGT]AGG
EagI 443 CGGCCG C^GGCCG
EarI 1492 CTCTTC CTCTTC(1/4)
EclXI 443 CGGCCG C^GGCCG
Eco47III 1537 AGCGCT AGC^GCT
Eco52I 443 CGGCCG C^GGCCG
Eco81I 101 CCTTAGG CC^T[ACGT]AGG
EcoO65I 276 GGTCACC G^GT[ACGT]ACC
HincII 1129 GTCAAC GT[CT]^[GA]AC
HindII 1129 GTCAAC GT[CT]^[GA]AC
KasI 294 GGCGCC G^GCGCC
Ksp632I 1492 CTCTTC CTCTTC(1/4)
NarI 294 GGCGCC GG^CGCC
NcoI 1281 CCATGG C^CATGG
NheI 1534 GCTAGC G^CTAGC
NruI 1197 TCGCGA TCG^CGA
PflMI 16 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 946 ACCGGT A^CCGGT
PshAI 338 GACTAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 377 ACCTGGT A^CC[AT]GGT
SfoI 294 GGCGCC GGC^GCC
SgrAI 945 CACCGGTG C[GA]^CCGG[CT]G
SpeI 339 ACTAGT A^CTAGT
SphI 410 GCATGC GCATG^C
StuI 812 AGGCCT AGG^CCT
TfiI 726 GATTC G^A[AT]TC
Van91I 16 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 189 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 942 GTGCAC G^TGCAC
1057 GTGCAC
AlwNI 17 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1385 CAGACCCTG
ApaLI 942 GTGCAC G^TGCAC
1057 GTGCAC
BfaI 340 CTAG C^TAG
1535 CTAG
BglI 326 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1652 GCCTTGTGGGC
BpmI 1105 CTGGAG CTGGAG(16/14)
1426 CTGGAG
BseRI 120 CTCCTC GAGGAG(10/8)
955 GAGGAG
BsmAI 1579 GAGAC GTCTC(1/5)
249 GTCTC
BspMI 1190 ACCTGC ACCTGC(4/8)
1508 ACCTGC
BsrBI 438 CCGCTC CCGCTC(-3/-3)
718 GAGCGG
BstYI 800 GGATCT [GA]^GATC[CT]
852 GGATCC
MaeI 340 CTAG C^TAG
1535 CTAG
MflI 800 GGATCT [GA]^GATC[CT]
852 GGATCC
MseI 665 TTAA T^TAA
923 TTAA
MslI 786 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
996 CATATCAGTG
MspA1I 1144 CAGCGG C[AC]G^C[GT]G
1551 CAGCGG
NspI 410 GCATGC [GA]CATG^[CT]
1667 ACATGT
SacI 502 GAGCTC GAGCT^C
958 GAGCTC
Tru9I 665 TTAA T^TAA
923 TTAA
Tsp509I 158 AATT ^AATT
921 AATT
XhoII 800 GGATCT [GA]^GATC[CT]
852 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TseI Tsp45I
TspRI TthHB8I XmaCI XmaI