Restriction map for
pFN21AE3270
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI Aor51HI AscI
AseI AsnI Asp700I AviII AvrII
BbeI BbvCI BciVI BclI BfrI
BlnI BsaBI BsaHI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII DrdI Eco47III EcoRI EcoRV
FbaI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MroI MseI
MunI NarI NdeI NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 1568 GGTACC G^GTACC
AcsI 855 GAATTT [GA]^AATT[CT]
AhdI 1069 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 397 CCTGAGG CC^T[ACGT]AGG
ApoI 855 GAATTT [GA]^AATT[CT]
Asp718I 1568 GGTACC G^GTACC
AspEI 1069 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1375 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 257 TGGCCA TGG^CCA
BbrPI 826 CACGTG CAC^GTG
BcgI 288 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 424 CTAG C^TAG
BsaAI 826 CACGTG [CT]AC^GT[GA]
BseRI 1555 GAGGAG GAGGAG(10/8)
BsiEI 1113 CGGCCG CG[GA][CT]^CG
BsmI 37 GAATGC GAATGC(1/-1)
BsrFI 65 GCCGGC [GA]^CCGG[CT]
BssSI 229 CACGAG CACGAG(-5/-1)
BstAPI 218 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1635 GGTCACC G^GT[ACGT]ACC
BstPI 1635 GGTCACC G^GT[ACGT]ACC
BstXI 134 CCATGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 397 CCTGAGG CC^T[ACGT]AGG
Cfr10I 65 GCCGGC [GA]^CCGG[CT]
EagI 1113 CGGCCG C^GGCCG
Eam1105I 1069 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1113 CGGCCG C^GGCCG
Eco52I 1113 CGGCCG C^GGCCG
Eco81I 397 CCTGAGG CC^T[ACGT]AGG
EcoNI 1080 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1635 GGTCACC G^GT[ACGT]ACC
EcoT22I 192 ATGCAT ATGCA^T
FseI 64 GGCCGGCC GGCCGG^CC
KpnI 1568 GGTACC GGTAC^C
MaeI 424 CTAG C^TAG
MluNI 257 TGGCCA TGG^CCA
MscI 257 TGGCCA TGG^CCA
NaeI 65 GCCGGC GCC^GGC
NcoI 1506 CCATGG C^CATGG
NgoMIV 65 GCCGGC G^CCGGC
NsiI 192 ATGCAT ATGCA^T
PflFI 1375 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 826 CACGTG CAC^GTG
PmlI 826 CACGTG CAC^GTG
SfiI 450 GGCCACCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 555 AGGCCT AGG^CCT
Tth111I 1375 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 1029 CGCG CG^CG
1292 CGCG
Alw44I 800 GTGCAC G^TGCAC
1057 GTGCAC
ApaI 787 GGGCCC GGGCC^C
1349 GGGCCC
ApaLI 800 GTGCAC G^TGCAC
1057 GTGCAC
BamHI 414 GGATCC G^GATCC
1540 GGATCC
BanI 1568 GGTACC G^G[CT][GA]CC
1901 GGTGCC
BbsI 485 GAAGAC GAAGAC(2/6)
1255 GAAGAC
BglII 359 AGATCT A^GATCT
1262 AGATCT
BlpI 616 GCTCAGC GC^T[ACGT]AGC
774 GCTGAGC
Bpu1102I 616 GCTCAGC GC^T[ACGT]AGC
774 GCTGAGC
BpuAI 485 GAAGAC GAAGAC(2/6)
1255 GAAGAC
BsaI 1748 GAGACC GGTCTC(1/5)
733 GGTCTC
BsaWI 43 ACCGGA [AT]^CCGG[AT]
641 TCCGGT
BsmBI 348 GAGACG CGTCTC(1/5)
601 GAGACG
BstUI 1029 CGCG CG^CG
1292 CGCG
CelII 616 GCTCAGC GC^T[ACGT]AGC
774 GCTGAGC
DsaI 455 CCGTGG C^C[GA][CT]GG
1506 CCATGG
EaeI 257 TGGCCA [CT]^GGCC[GA]
1113 CGGCCG
HaeII 1198 AGCGCC [GA]GCGC^[CT]
1585 AGCGCC
MaeII 683 ACGT A^CGT
827 ACGT
MslI 822 CATTCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
864 CACGCTGATG
MvnI 1029 CGCG CG^CG
1292 CGCG
SmlI 913 CTCAAG C^T[CT][GA]AG
1063 CTCAAG
TaqI 206 TCGA T^CGA
590 TCGA
TthHB8I 206 TCGA T^CGA
590 TCGA
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmaI StyI TfiI
TseI Tsp45I Tsp509I TspRI XcmI
XhoII XmaCI XmaI