Restriction map for
pFN21AE9602
##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BpuAI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HapII HgaI HindIII HpaI
HpaII KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MspI MunI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 373 GTCGAC GT^[AC][GT]AC
AcsI 547 AAATTC [GA]^AATT[CT]
AfaI 411 GTAC GT^AC
Alw44I 217 GTGCAC G^TGCAC
ApaLI 217 GTGCAC G^TGCAC
ApoI 547 AAATTC [GA]^AATT[CT]
BanII 328 GAGCTC G[GA]GC[CT]^C
BglI 47 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 42 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 42 GCTCAGC GC^T[ACGT]AGC
BsaAI 412 TACGTG [CT]AC^GT[GA]
BsiEI 404 CGGCCG CG[GA][CT]^CG
BsrBI 115 GAGCGG CCGCTC(-3/-3)
BsrI 500 CCAGT ACTGG(1/-1)
BssSI 100 CACGAG CACGAG(-5/-1)
CelII 42 GCTCAGC GC^T[ACGT]AGC
DdeI 43 CTCAG C^T[ACGT]AG
DraIII 582 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 382 GACACGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 170 CCATGG C^C[GA][CT]GG
EagI 404 CGGCCG C^GGCCG
EarI 325 GAAGAG CTCTTC(1/4)
EclXI 404 CGGCCG C^GGCCG
Eco52I 404 CGGCCG C^GGCCG
EcoT14I 170 CCATGG C^C[AT][AT]GG
HincII 373 GTCGAC GT[CT]^[GA]AC
HindII 373 GTCGAC GT[CT]^[GA]AC
HinfI 570 GAGTC G^A[ACGT]TC
Ksp632I 325 GAAGAG CTCTTC(1/4)
MaeII 413 ACGT A^CGT
MaeIII 391 GTCAC ^GT[ACGT]AC
MboII 325 GAAGA GAAGA(8/7)
NcoI 170 CCATGG C^CATGG
PleI 570 GAGTC GAGTC(4/5)
PshAI 280 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 238 CTGCAG CTGCA^G
RsaI 411 GTAC GT^AC
SacI 328 GAGCTC GAGCT^C
SalI 373 GTCGAC G^TCGAC
SapI 325 GAAGAGC GCTCTTC(1/4)
SfaNI 485 GCATC GCATC(5/9)
SfcI 238 CTGCAG C^T[GA][CT]AG
SmlI 331 CTCAAG C^T[CT][GA]AG
StyI 170 CCATGG C^C[AT][AT]GG
TaqI 374 TCGA T^CGA
Tsp45I 391 GTCAC ^GT[GC]AC
TspRI 501 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 374 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 55 GGCGCC G[GA]^CG[CT]C
95 GGCGCC
AlwI 162 GATCC GGATC(4/5)
348 GGATC
AlwNI 34 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
241 CAGCAGCTG
AspHI 217 GTGCAC G[AT]GC[AT]^C
328 GAGCTC
BbeI 55 GGCGCC GGCGC^C
95 GGCGCC
BmyI 217 GTGCAC G[AGT]GC[ACT]^C
328 GAGCTC
BsaHI 55 GGCGCC G[GA]^CG[CT]C
95 GGCGCC
BseRI 22 GAGGAG GAGGAG(10/8)
274 GAGGAG
BsiHKAI 217 GTGCAC G[AT]GC[AT]^C
328 GAGCTC
Bsp1286I 217 GTGCAC G[AGT]GC[ACT]^C
328 GAGCTC
BspMI 105 GCAGGT ACCTGC(4/8)
213 GCAGGT
BstXI 314 CCAGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
515 CCATCCACCTGG
BstYI 161 AGATCC [GA]^GATC[CT]
348 GGATCT
Cac8I 47 GCCTGC GC[ACGT]^[ACGT]GC
295 GCCAGC
DpnII 162 GATC ^GATC
349 GATC
EaeI 2 TGGCCG [CT]^GGCC[GA]
404 CGGCCG
HaeII 55 GGCGCC [GA]GCGC^[CT]
95 GGCGCC
Hin1I 55 GGCGCC G[GA]^CG[CT]C
95 GGCGCC
KasI 55 GGCGCC G^GCGCC
95 GGCGCC
MboI 162 GATC ^GATC
349 GATC
MflI 161 AGATCC [GA]^GATC[CT]
348 GGATCT
MspA1I 151 CAGCTG C[AC]G^C[GT]G
244 CAGCTG
NarI 55 GGCGCC GG^CGCC
95 GGCGCC
NdeII 162 GATC ^GATC
349 GATC
NlaIII 171 CATG CATG^
493 CATG
PvuII 151 CAGCTG CAG^CTG
244 CAGCTG
Sau3AI 162 GATC ^GATC
349 GATC
SfoI 55 GGCGCC GGC^GCC
95 GGCGCC
Tsp509I 444 AATT ^AATT
548 AATT
XhoII 161 AGATCC [GA]^GATC[CT]
348 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AccII AciI AluI BanI BbvI
BpmI BsaJI BsiYI BslI BssKI
BstF5I BstNI BstUI CfoI Cfr13I
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HphI ItaI MnlI MvaI
MvnI MwoI NlaIV Sau96I ScrFI
TseI