Restriction map for
pFN21AE5486
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AvaI AviII AvrII BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I CelII
CfoI ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KspI MaeI
MaeII MamI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 827 GGTACC G^GTACC
AcsI 85 GAATTC [GA]^AATT[CT]
AgeI 416 ACCGGT A^CCGGT
ApoI 85 GAATTC [GA]^AATT[CT]
Asp700I 909 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 827 GGTACC G^GTACC
BalI 425 TGGCCA TGG^CCA
BglI 470 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 950 GTGCCC G[AGT]GC[ACT]^C
BsaWI 416 ACCGGT [AT]^CCGG[AT]
Bsp1286I 950 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 884 TGTACA T^GTACA
BspHI 458 TCATGA T^CATGA
BsrGI 884 TGTACA T^GTACA
BsrI 522 CCAGT ACTGG(1/-1)
BstEII 894 GGTCACC G^GT[ACGT]ACC
BstPI 894 GGTCACC G^GT[ACGT]ACC
BstXI 152 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 959 GCAGGC GC[ACGT]^[ACGT]GC
DrdI 348 GACTCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 281 CCACGG C^C[GA][CT]GG
EaeI 425 TGGCCA [CT]^GGCC[GA]
EarI 861 GAAGAG CTCTTC(1/4)
EcoNI 682 CCTATCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 894 GGTCACC G^GT[ACGT]ACC
EcoRI 85 GAATTC G^AATTC
EcoT14I 254 CCTTGG C^C[AT][AT]GG
KpnI 827 GGTACC GGTAC^C
Ksp632I 861 GAAGAG CTCTTC(1/4)
MluNI 425 TGGCCA TGG^CCA
MscI 425 TGGCCA TGG^CCA
MseI 484 TTAA T^TAA
MspA1I 839 CAGCTG C[AC]G^C[GT]G
MwoI 470 GCCTCTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PinAI 416 ACCGGT A^CCGGT
PstI 121 CTGCAG CTGCA^G
PvuII 839 CAGCTG CAG^CTG
RcaI 458 TCATGA T^CATGA
SexAI 158 ACCTGGT A^CC[AT]GGT
SspBI 884 TGTACA T^GTACA
StyI 254 CCTTGG C^C[AT][AT]GG
Tru9I 484 TTAA T^TAA
XmnI 909 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AocI 612 CCTTAGG CC^T[ACGT]AGG
927 CCTGAGG
AvaII 129 GGTCC G^G[AT]CC
285 GGTCC
BamHI 111 GGATCC G^GATCC
692 GGATCC
BanI 661 GGTGCC G^G[CT][GA]CC
827 GGTACC
BseRI 91 CTCCTC GAGGAG(10/8)
604 CTCCTC
BsgI 442 CTGCAC GTGCAG(16/14)
957 GTGCAG
BsmAI 79 GTCTC GTCTC(1/5)
677 GTCTC
BsmFI 99 GGGAC GGGAC(10/14)
286 GTCCC
BspMI 179 ACCTGC ACCTGC(4/8)
553 ACCTGC
BsrFI 126 GCCGGT [GA]^CCGG[CT]
416 ACCGGT
BstYI 111 GGATCC [GA]^GATC[CT]
692 GGATCC
Bsu36I 612 CCTTAGG CC^T[ACGT]AGG
927 CCTGAGG
Cfr10I 126 GCCGGT [GA]^CCGG[CT]
416 ACCGGT
Cfr13I 129 GGTCC G^G[ACGT]CC
285 GGTCC
DpnII 112 GATC ^GATC
693 GATC
Eco81I 612 CCTTAGG CC^T[ACGT]AGG
927 CCTGAGG
HaeIII 408 GGCC GG^CC
426 GGCC
HapII 127 CCGG C^CGG
417 CCGG
HpaII 127 CCGG C^CGG
417 CCGG
MboI 112 GATC ^GATC
693 GATC
MflI 111 GGATCC [GA]^GATC[CT]
692 GGATCC
MslI 222 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
897 CACCCCCATG
MspI 127 CCGG C^CGG
417 CCGG
NdeII 112 GATC ^GATC
693 GATC
Sau3AI 112 GATC ^GATC
693 GATC
Sau96I 129 GGTCC G^G[ACGT]CC
285 GGTCC
SfcI 121 CTGCAG C^T[GA][CT]AG
921 CTACAG
TspRI 878 CAGTG CA[GC]TG[ACGT][ACGT]^
888 CACTG
XhoII 111 GGATCC [GA]^GATC[CT]
692 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI AlwI BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
DdeI EcoRII Fnu4HI FokI HinfI
HphI ItaI MaeIII MboII MnlI
MvaI NlaIII NlaIV NspI PleI
RsaI ScrFI TfiI TseI Tsp45I
Tsp509I XcmI