Restriction map for
pFN21AE2201
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbrPI BbsI BfaI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BseAI BsiWI BsmBI Bsp1407I BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
CpoI DraI DraIII DrdI Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FspI HincII HindII HindIII HpaI
KpnI MaeI MluI MroI NcoI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SapI SbfI
SexAI SfuI SmlI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AocI 1176 CCTCAGG CC^T[ACGT]AGG
BalI 1973 TGGCCA TGG^CCA
BamHI 861 GGATCC G^GATCC
BbeI 73 GGCGCC GGCGC^C
BbvCI 1822 CCTCAGC CCTCAGC(-5/-2)
BcgI 1098 CGAGAAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1064 GGATAC GTATCC(6/5)
BglII 1376 AGATCT A^GATCT
BsaI 760 GAGACC GGTCTC(1/5)
BsmI 993 GCATTC GAATGC(1/-1)
BspDI 973 ATCGAT AT^CGAT
Bsu36I 1176 CCTCAGG CC^T[ACGT]AGG
ClaI 973 ATCGAT AT^CGAT
Eco81I 1176 CCTCAGG CC^T[ACGT]AGG
FseI 732 GGCCGGCC GGCCGG^CC
KasI 73 GGCGCC G^GCGCC
MaeII 915 ACGT A^CGT
MaeIII 917 GTGAC ^GT[ACGT]AC
MfeI 1254 CAATTG C^AATTG
MluNI 1973 TGGCCA TGG^CCA
MscI 1973 TGGCCA TGG^CCA
MunI 1254 CAATTG C^AATTG
NarI 73 GGCGCC GG^CGCC
NotI 1702 GCGGCCGC GC^GGCCGC
PflMI 2077 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 382 GACTC GAGTC(4/5)
SacI 1994 GAGCTC GAGCT^C
ScaI 1949 AGTACT AGT^ACT
SfoI 73 GGCGCC GGC^GCC
SgrAI 48 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1214 GCATGC GCATG^C
SspI 2039 AATATT AAT^ATT
StuI 1859 AGGCCT AGG^CCT
Tsp45I 917 GTGAC ^GT[GC]AC
Van91I 2077 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 73 GGCGCC G[GA]^CG[CT]C
226 GACGCC
AhdI 628 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2073 GACCCCAGGTC
AspEI 628 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2073 GACCCCAGGTC
AspHI 1138 GAGCAC G[AT]GC[AT]^C
1994 GAGCTC
BanI 73 GGCGCC G^G[CT][GA]CC
697 GGCACC
BclI 473 TGATCA T^GATCA
1031 TGATCA
BsaHI 73 GGCGCC G[GA]^CG[CT]C
226 GACGCC
BsiHKAI 1138 GAGCAC G[AT]GC[AT]^C
1994 GAGCTC
BspHI 713 TCATGA T^CATGA
1677 TCATGA
DsaI 722 CCGCGG C^C[GA][CT]GG
1706 CCGCGG
Eam1105I 628 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2073 GACCCCAGGTC
FbaI 473 TGATCA T^GATCA
1031 TGATCA
HaeII 73 GGCGCC [GA]GCGC^[CT]
1911 AGCGCC
HgaI 214 GACGC GACGC(5/10)
226 GACGC
Hin1I 73 GGCGCC G[GA]^CG[CT]C
226 GACGCC
KspI 722 CCGCGG CCGC^GG
1706 CCGCGG
NaeI 49 GCCGGC GCC^GGC
733 GCCGGC
NgoMIV 49 GCCGGC G^CCGGC
733 GCCGGC
NspI 1214 GCATGC [GA]CATG^[CT]
1596 ACATGC
PstI 775 CTGCAG CTGCA^G
1697 CTGCAG
RcaI 713 TCATGA T^CATGA
1677 TCATGA
SacII 722 CCGCGG CCGC^GG
1706 CCGCGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BanII
BbvI BcnI BglI BmyI BpmI
BsaBI BsaJI BsaWI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SmaI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI