Restriction map for
pFN21AE1249
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BbrPI
BbsI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I EarI
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HinfI
HpaI KpnI Ksp632I MaeI MaeII
MfeI MluI MluNI MroI MscI
MseI MunI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsaI RsrII SalI SapI
SbfI ScaI SexAI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Aor51HI 1004 AGCGCT AGC^GCT
AspHI 1360 GAGCTC G[AT]GC[AT]^C
AvaII 556 GGACC G^G[AT]CC
AviII 1079 TGCGCA TGC^GCA
BamHI 1040 GGATCC G^GATCC
BbvCI 1420 GCTGAGG CCTCAGC(-5/-2)
BcgI 1280 GCACGCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 13 CTGGAG CTGGAG(16/14)
BsaBI 1287 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1120 GAGACC GGTCTC(1/5)
BsiHKAI 1360 GAGCTC G[AT]GC[AT]^C
BsmAI 1120 GAGAC GTCTC(1/5)
BspHI 1385 TCATGA T^CATGA
BsrDI 271 GCAATG GCAATG(2/0)
BstEII 1332 GGTCACC G^GT[ACGT]ACC
BstPI 1332 GGTCACC G^GT[ACGT]ACC
BstYI 1040 GGATCC [GA]^GATC[CT]
DraIII 34 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1004 AGCGCT AGC^GCT
EcoNI 1124 CCTTCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1332 GGTCACC G^GT[ACGT]ACC
FspI 1079 TGCGCA TGC^GCA
MaeIII 1333 GTCAC ^GT[ACGT]AC
MamI 1287 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1040 GGATCC [GA]^GATC[CT]
MslI 504 CACCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 581 CCATGG C^CATGG
NspI 1169 GCATGT [GA]CATG^[CT]
PflMI 1088 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1385 TCATGA T^CATGA
SacI 1360 GAGCTC GAGCT^C
SfiI 685 GGCCCGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1452 CGCCGGCG C[GA]^CCGG[CT]G
TaqI 734 TCGA T^CGA
Tsp45I 1333 GTCAC ^GT[GC]AC
TspRI 773 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 734 TCGA T^CGA
Van91I 1088 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1040 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 1041 GATCC GGATC(4/5)
1040 GGATC
ApaI 255 GGGCCC GGGCC^C
1446 GGGCCC
BlpI 6 GCTGAGC GC^T[ACGT]AGC
66 GCTGAGC
Bpu1102I 6 GCTGAGC GC^T[ACGT]AGC
66 GCTGAGC
BseRI 955 GAGGAG GAGGAG(10/8)
1423 GAGGAG
BsmFI 707 GGGAC GGGAC(10/14)
756 GGGAC
BsrFI 142 GCCGGC [GA]^CCGG[CT]
1453 GCCGGC
BsrI 249 ACTGG ACTGG(1/-1)
774 ACTGG
BssHII 82 GCGCGC G^CGCGC
1227 GCGCGC
BstAPI 278 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
333 GCAGCACCTGC
BstXI 696 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
968 CCAAGGAGGTGG
CelII 6 GCTGAGC GC^T[ACGT]AGC
66 GCTGAGC
Cfr10I 142 GCCGGC [GA]^CCGG[CT]
1453 GCCGGC
NaeI 142 GCCGGC GCC^GGC
1453 GCCGGC
NgoMIV 142 GCCGGC G^CCGGC
1453 GCCGGC
NotI 385 GCGGCCGC GC^GGCCGC
442 GCGGCCGC
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI BanI BanII BbeI BbvI
BcnI BglI BmyI BsaHI BsaJI
BsgI BsiEI BsiYI BslI BsoBI
Bsp1286I BspMI BsrBI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
KspI MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PstI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StyI TseI
XmaCI XmaI