Restriction map for
pFN21AE4017
##################################
# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BciVI BclI
BfaI BfrI BglI BglII BlnI
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HinfI
HpaI KpnI MaeI MaeII MfeI
MflI MluI MluNI MroI MscI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RcaI RsaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tsp45I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccII 31 CGCG CG^CG
AflIII 437 ACATGT A^C[GA][CT]GT
ApaI 261 GGGCCC GGGCC^C
AseI 462 ATTAAT AT^TAAT
AsnI 462 ATTAAT AT^TAAT
AspHI 138 GAGCTC G[AT]GC[AT]^C
AvaI 143 CCCGGG C^[CT]CG[GA]G
AvaII 272 GGTCC G^G[AT]CC
BbeI 34 GGCGCC GGCGC^C
BbsI 105 GAAGAC GAAGAC(2/6)
BcgI 388 GCAGAAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 542 GCTTAGC GC^T[ACGT]AGC
BpmI 565 CTCCAG CTGGAG(16/14)
Bpu1102I 542 GCTTAGC GC^T[ACGT]AGC
BpuAI 105 GAAGAC GAAGAC(2/6)
BsaBI 231 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 364 GGTCTC GGTCTC(1/5)
BseRI 448 GAGGAG GAGGAG(10/8)
BsgI 352 GTGCAG GTGCAG(16/14)
BsiHKAI 138 GAGCTC G[AT]GC[AT]^C
BsoBI 143 CCCGGG C^[CT]CG[GA]G
BspLU11I 437 ACATGT A^CATGT
BsrDI 483 CATTGC GCAATG(2/0)
BsrFI 10 GCCGGT [GA]^CCGG[CT]
BstUI 31 CGCG CG^CG
CelII 542 GCTTAGC GC^T[ACGT]AGC
Cfr10I 10 GCCGGT [GA]^CCGG[CT]
DpnII 231 GATC ^GATC
DraII 261 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 8 TGGCCG [CT]^GGCC[GA]
EarI 409 CTCTTC CTCTTC(1/4)
EcoO109I 261 GGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 18 GCGGG CCCGC(4/6)
KasI 34 GGCGCC G^GCGCC
Ksp632I 409 CTCTTC CTCTTC(1/4)
KspI 30 CCGCGG CCGC^GG
MamI 231 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 231 GATC ^GATC
MseI 463 TTAA T^TAA
MspA1I 30 CCGCGG C[AC]G^C[GT]G
MvnI 31 CGCG CG^CG
NarI 34 GGCGCC GG^CGCC
NdeII 231 GATC ^GATC
NspI 437 ACATGT [GA]CATG^[CT]
PshBI 462 ATTAAT AT^TAAT
SacI 138 GAGCTC GAGCT^C
SacII 30 CCGCGG CCGC^GG
Sau3AI 231 GATC ^GATC
SfoI 34 GGCGCC GGC^GCC
SmaI 143 CCCGGG CCC^GGG
SmlI 24 CTTGAG C^T[CT][GA]AG
TaqI 422 TCGA T^CGA
Tru9I 463 TTAA T^TAA
TspRI 537 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 422 TCGA T^CGA
XmaCI 143 CCCGGG C^CCGGG
XmaI 143 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BanII 138 GAGCTC G[GA]GC[CT]^C
261 GGGCCC
BsmAI 77 GTCTC GTCTC(1/5)
365 GTCTC
BsrI 538 ACTGG ACTGG(1/-1)
275 CCAGT
Cac8I 18 GCGGGC GC[ACGT]^[ACGT]GC
555 GCTGGC
CfoI 35 GCGC GCG^C
524 GCGC
DdeI 360 CTTAG C^T[ACGT]AG
543 CTTAG
HaeII 34 GGCGCC [GA]GCGC^[CT]
523 AGCGCC
HhaI 35 GCGC GCG^C
524 GCGC
HinP1I 35 GCGC G^CGC
524 GCGC
MaeIII 278 GTTAC ^GT[ACGT]AC
434 GTTAC
MboII 105 GAAGA GAAGA(8/7)
410 TCTTC
MwoI 42 GCTACGGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
555 GCTGGCCTGGC
NlaIII 258 CATG CATG^
438 CATG
PstI 127 CTGCAG CTGCA^G
374 CTGCAG
SfaNI 343 GCATC GCATC(5/9)
394 GCATC
SfcI 127 CTGCAG C^T[GA][CT]AG
374 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AcyI AluI BanI BbvI
BcnI BmyI BsaHI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cfr13I DsaI EcoRII Fnu4HI FokI
HaeIII HapII HgaI Hin1I HpaII
HphI ItaI MnlI MspI MvaI
NciI NlaIV Sau96I ScrFI TseI