Restriction map for
pFN21AE2441
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# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI ApoI AscI AseI
AsnI Asp718I AspEI BcgI BciVI
BfrI BglII BsiWI BsmBI BsmI
BsrBI BssHII Bst1107I BstBI BstZ17I
CpoI DraI Eam1105I EcoRI EcoRV
HpaI KpnI MfeI MluI MunI
NheI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
RsrII SfuI SgrAI SnaBI SpeI
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 547 GTCGAC GT^[AC][GT]AC
AccIII 4403 TCCGGA T^CCGGA
Alw44I 18 GTGCAC G^TGCAC
ApaLI 18 GTGCAC G^TGCAC
AspI 3205 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3059 TGCGCA TGC^GCA
AvrII 3459 CCTAGG C^CTAGG
BamHI 249 GGATCC G^GATCC
BbeI 3463 GGCGCC GGCGC^C
BclI 413 TGATCA T^GATCA
BlnI 3459 CCTAGG C^CTAGG
BsaWI 4403 TCCGGA [AT]^CCGG[AT]
BseAI 4403 TCCGGA T^CCGGA
BsgI 2995 GTGCAG GTGCAG(16/14)
BspDI 4537 ATCGAT AT^CGAT
BspEI 4403 TCCGGA T^CCGGA
ClaI 4537 ATCGAT AT^CGAT
EagI 4604 CGGCCG C^GGCCG
EclXI 4604 CGGCCG C^GGCCG
Eco52I 4604 CGGCCG C^GGCCG
EcoT22I 2230 ATGCAT ATGCA^T
FbaI 413 TGATCA T^GATCA
FseI 2316 GGCCGGCC GGCCGG^CC
FspI 3059 TGCGCA TGC^GCA
HindIII 3973 AAGCTT A^AGCTT
KasI 3463 GGCGCC G^GCGCC
KspI 4386 CCGCGG CCGC^GG
MroI 4403 TCCGGA T^CCGGA
NarI 3463 GGCGCC GG^CGCC
NsiI 2230 ATGCAT ATGCA^T
PaeR7I 2719 CTCGAG C^TCGAG
PflFI 3205 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 713 CGATCG CGAT^CG
SacI 2406 GAGCTC GAGCT^C
SacII 4386 CCGCGG CCGC^GG
SalI 547 GTCGAC G^TCGAC
SapI 810 GCTCTTC GCTCTTC(1/4)
SexAI 1841 ACCTGGT A^CC[AT]GGT
SfoI 3463 GGCGCC GGC^GCC
SphI 2153 GCATGC GCATG^C
TfiI 3425 GATTC G^A[AT]TC
Tth111I 3205 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2719 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Aor51HI 2999 AGCGCT AGC^GCT
5231 AGCGCT
BbvCI 1953 CCTCAGC CCTCAGC(-5/-2)
4950 GCTGAGG
BsiEI 713 CGATCG CG[GA][CT]^CG
4604 CGGCCG
Bsp1407I 4154 TGTACA T^GTACA
4775 TGTACA
BsrGI 4154 TGTACA T^GTACA
4775 TGTACA
BstEII 2101 GGTAACC G^GT[ACGT]ACC
4101 GGTCACC
BstPI 2101 GGTAACC G^GT[ACGT]ACC
4101 GGTCACC
DrdI 589 GACTTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4258 GACTTCGTGGTC
Eco47III 2999 AGCGCT AGC^GCT
5231 AGCGCT
EcoO65I 2101 GGTAACC G^GT[ACGT]ACC
4101 GGTCACC
MseI 2735 TTAA T^TAA
3266 TTAA
NdeI 1346 CATATG CA^TATG
1668 CATATG
SbfI 3711 CCTGCAGG CCTGCA^GG
4938 CCTGCAGG
ScaI 4226 AGTACT AGT^ACT
4316 AGTACT
SfiI 1329 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1365 GGCCACCCTGGCC
Sse8387I 3711 CCTGCAGG CCTGCA^GG
4938 CCTGCAGG
SspBI 4154 TGTACA T^GTACA
4775 TGTACA
Tru9I 2735 TTAA T^TAA
3266 TTAA
Tsp509I 2083 AATT ^AATT
4002 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspLU11I BspMI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI