Restriction map for
pFN21AB5820
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BbrPI BcgI
BciVI BclI BfrI BlnI BsaAI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HgaI HpaI
KpnI MfeI MluI MluNI MroI
MscI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SacI SapI SbfI
ScaI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 1183 GTCGAC GT^[AC][GT]AC
AcsI 1580 GAATTC [GA]^AATT[CT]
AcyI 55 GGCGCC G[GA]^CG[CT]C
AocI 1465 CCTGAGG CC^T[ACGT]AGG
ApoI 1580 GAATTC [GA]^AATT[CT]
AspI 131 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 160 CCCGGG C^[CT]CG[GA]G
BamHI 336 GGATCC G^GATCC
BbeI 55 GGCGCC GGCGC^C
BbvCI 436 CCTCAGC CCTCAGC(-5/-2)
BfaI 725 CTAG C^TAG
BglII 1082 AGATCT A^GATCT
BlpI 39 GCTAAGC GC^T[ACGT]AGC
BpmI 872 CTGGAG CTGGAG(16/14)
Bpu1102I 39 GCTAAGC GC^T[ACGT]AGC
BsaBI 531 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 55 GGCGCC G[GA]^CG[CT]C
BsmBI 1476 GAGACG CGTCTC(1/5)
BsoBI 160 CCCGGG C^[CT]CG[GA]G
BspDI 1222 ATCGAT AT^CGAT
BspMI 261 GCAGGT ACCTGC(4/8)
BsrDI 516 CATTGC GCAATG(2/0)
BsrFI 117 ACCGGC [GA]^CCGG[CT]
BssSI 1042 CACGAG CACGAG(-5/-1)
Bsu36I 1465 CCTGAGG CC^T[ACGT]AGG
CelII 39 GCTAAGC GC^T[ACGT]AGC
Cfr10I 117 ACCGGC [GA]^CCGG[CT]
ClaI 1222 ATCGAT AT^CGAT
CpoI 114 CGGACCG CG^G[AT]CCG
DraIII 808 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1095 TGGCCG [CT]^GGCC[GA]
EarI 735 GAAGAG CTCTTC(1/4)
Eco81I 1465 CCTGAGG CC^T[ACGT]AGG
EcoRI 1580 GAATTC G^AATTC
Hin1I 55 GGCGCC G[GA]^CG[CT]C
HincII 1183 GTCGAC GT[CT]^[GA]AC
HindII 1183 GTCGAC GT[CT]^[GA]AC
HindIII 577 AAGCTT A^AGCTT
KasI 55 GGCGCC G^GCGCC
Ksp632I 735 GAAGAG CTCTTC(1/4)
MaeI 725 CTAG C^TAG
MamI 531 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 55 GGCGCC GG^CGCC
PflFI 131 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 114 CGGACCG CG^G[AT]CCG
SalI 1183 GTCGAC G^TCGAC
SexAI 809 ACCTGGT A^CC[AT]GGT
SfiI 187 GGCCCCGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 55 GGCGCC GGC^GCC
SmaI 160 CCCGGG CCC^GGG
SmlI 1435 CTCAAG C^T[CT][GA]AG
Tsp45I 228 GTCAC ^GT[GC]AC
Tth111I 131 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 160 CCCGGG C^CCGGG
XmaI 160 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 891 GTGCAC G^TGCAC
967 GTGCAC
ApaLI 891 GTGCAC G^TGCAC
967 GTGCAC
AspHI 891 GTGCAC G[AT]GC[AT]^C
967 GTGCAC
BanI 55 GGCGCC G^G[CT][GA]CC
821 GGCACC
BbsI 1203 GAAGAC GAAGAC(2/6)
1350 GAAGAC
BpuAI 1203 GAAGAC GAAGAC(2/6)
1350 GAAGAC
BsaWI 239 ACCGGA [AT]^CCGG[AT]
824 ACCGGA
BsiHKAI 891 GTGCAC G[AT]GC[AT]^C
967 GTGCAC
BsrBI 496 GAGCGG CCGCTC(-3/-3)
1045 GAGCGG
BsrI 1563 ACTGG ACTGG(1/-1)
927 CCAGT
BstAPI 634 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
822 GCACCGGATGC
HphI 229 TCACC GGTGA(8/7)
807 TCACC
MaeII 601 ACGT A^CGT
884 ACGT
MseI 403 TTAA T^TAA
758 TTAA
NcoI 182 CCATGG C^CATGG
248 CCATGG
PvuII 1027 CAGCTG CAG^CTG
1345 CAGCTG
SphI 104 GCATGC GCATG^C
317 GCATGC
TfiI 443 GAATC G^A[AT]TC
1116 GATTC
Tru9I 403 TTAA T^TAA
758 TTAA
TspRI 1244 CACTG CA[GC]TG[ACGT][ACGT]^
1446 CACTG
XcmI 840 CCAGGAGACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1026 CCAGCTGCAGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaII BanII BbvI
BcnI BglI BmyI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
KspI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp509I TthHB8I XhoII