Restriction map for
pFN21AE1857
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BanII BbeI
BbrPI BcgI BclI BfrI BglII
BlnI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraII DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
MamI MluI MluNI MroI MscI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Alw44I 23 GTGCAC G^TGCAC
AlwNI 1027 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 23 GTGCAC G^TGCAC
AspHI 23 GTGCAC G[AT]GC[AT]^C
BamHI 529 GGATCC G^GATCC
BanI 309 GGCACC G^G[CT][GA]CC
BbsI 91 GTCTTC GAAGAC(2/6)
BbvCI 533 CCTCAGC CCTCAGC(-5/-2)
BbvI 51 GCAGC GCAGC(8/12)
BciVI 173 GTATCC GTATCC(6/5)
BcnI 1083 CCGGG CC^[GC]GG
BfaI 314 CTAG C^TAG
BglI 139 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 553 CTGGAG CTGGAG(16/14)
BpuAI 91 GTCTTC GAAGAC(2/6)
BsaAI 940 TACGTG [CT]AC^GT[GA]
BseRI 472 GAGGAG GAGGAG(10/8)
BsiEI 879 CGGCCG CG[GA][CT]^CG
BsiHKAI 23 GTGCAC G[AT]GC[AT]^C
BsmFI 110 GGGAC GGGAC(10/14)
BssHII 85 GCGCGC G^CGCGC
BstAPI 1100 GCAAATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 432 TTCGAA TT^CGAA
BstXI 252 CCACGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 907 TTTAAA TTT^AAA
DsaI 140 CCGCGG C^C[GA][CT]GG
EagI 879 CGGCCG C^GGCCG
EclXI 879 CGGCCG C^GGCCG
Eco52I 879 CGGCCG C^GGCCG
EcoRI 295 GAATTC G^AATTC
EcoT14I 749 CCAAGG C^C[AT][AT]GG
HgaI 89 GCGTC GACGC(5/10)
KspI 140 CCGCGG CCGC^GG
MaeI 314 CTAG C^TAG
MaeII 941 ACGT A^CGT
MfeI 794 CAATTG C^AATTG
MunI 794 CAATTG C^AATTG
NaeI 4 GCCGGC GCC^GGC
NciI 1083 CCGGG CC^[GC]GG
NgoMIV 4 GCCGGC G^CCGGC
NlaIII 891 CATG CATG^
NspV 432 TTCGAA TT^CGAA
PleI 617 GACTC GAGTC(4/5)
SacII 140 CCGCGG CCGC^GG
SfuI 432 TTCGAA TT^CGAA
StyI 749 CCAAGG C^C[AT][AT]GG
TaqI 433 TCGA T^CGA
TfiI 1092 GATTC G^A[AT]TC
TseI 51 GCAGC G^C[AT]GC
Tsp45I 497 GTGAC ^GT[GC]AC
TthHB8I 433 TCGA T^CGA
XcmI 1004 CCACTGCTGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AfaI 367 GTAC GT^AC
1080 GTAC
Asp700I 234 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1018 GAAAAAGTTC
AvaII 394 GGACC G^G[AT]CC
663 GGTCC
BlpI 831 GCTGAGC GC^T[ACGT]AGC
895 GCTCAGC
BmyI 23 GTGCAC G[AGT]GC[ACT]^C
745 GTGCCC
Bpu1102I 831 GCTGAGC GC^T[ACGT]AGC
895 GCTCAGC
Bsp1286I 23 GTGCAC G[AGT]GC[ACT]^C
745 GTGCCC
BspMI 27 ACCTGC ACCTGC(4/8)
1050 GCAGGT
BsrFI 4 GCCGGC [GA]^CCGG[CT]
65 GCCGGT
BstF5I 390 GGATG GGATG(2/0)
857 GGATG
BstYI 78 AGATCC [GA]^GATC[CT]
529 GGATCC
CelII 831 GCTGAGC GC^T[ACGT]AGC
895 GCTCAGC
CfoI 85 GCGC GCG^C
87 GCGC
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
65 GCCGGT
Cfr13I 394 GGACC G^G[ACGT]CC
663 GGTCC
EaeI 2 TGGCCG [CT]^GGCC[GA]
879 CGGCCG
FauI 180 GCGGG CCCGC(4/6)
854 GCGGG
FokI 390 GGATG GGATG(9/13)
857 GGATG
HaeIII 3 GGCC GG^CC
880 GGCC
HhaI 85 GCGC GCG^C
87 GCGC
HinP1I 85 GCGC G^CGC
87 GCGC
HinfI 617 GACTC G^A[ACGT]TC
1092 GATTC
MflI 78 AGATCC [GA]^GATC[CT]
529 GGATCC
MseI 403 TTAA T^TAA
908 TTAA
MslI 97 CACCACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
253 CACGAAAATG
MspA1I 140 CCGCGG C[AC]G^C[GT]G
178 CAGCGG
PstI 29 CTGCAG CTGCA^G
1048 CTGCAG
RsaI 367 GTAC GT^AC
1080 GTAC
SapI 130 GAAGAGC GCTCTTC(1/4)
435 GAAGAGC
Sau96I 394 GGACC G^G[ACGT]CC
663 GGTCC
SexAI 396 ACCTGGT A^CC[AT]GGT
1031 ACCTGGT
SfaNI 810 GATGC GCATC(5/9)
861 GATGC
Tru9I 403 TTAA T^TAA
908 TTAA
XhoII 78 AGATCC [GA]^GATC[CT]
529 GGATCC
XmnI 234 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1018 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI BsaJI BsiYI BslI BsrI
BssKI BstNI BstUI Cac8I DdeI
DpnII EarI EcoRII Fnu4HI HapII
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MspI MvaI
MvnI MwoI NdeII NlaIV Sau3AI
ScrFI SfcI Tsp509I TspRI