##################################
# NO CUT ENZYMES (  213 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TseI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AcsI          343  AAATTT               [GA]^AATT[CT]
AlwNI         197  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          343  AAATTT               [GA]^AATT[CT]
BpmI          292  CTGGAG               CTGGAG(16/14)
BsmAI         162  GTCTC                GTCTC(1/5)
BssKI         291  CCTGG                ^CC[ACGT]GG
BstF5I        100  CATCC                GGATG(2/0)
BstNI         291  CCTGG                CC^[AT]GG
Cac8I         184  GCCCGC               GC[ACGT]^[ACGT]GC
DdeI           44  CTGAG                C^T[ACGT]AG
DpnII          52  GATC                 ^GATC
DraI          190  TTTAAA               TTT^AAA
EarI          316  GAAGAG               CTCTTC(1/4)
EcoRII        291  CCTGG                ^CC[AT]GG
EcoRV         167  GATATC               GAT^ATC
FauI          185  CCCGC                CCCGC(4/6)
FokI          100  CATCC                GGATG(9/13)
HphI          301  TCACC                GGTGA(8/7)
Ksp632I       316  GAAGAG               CTCTTC(1/4)
MboI           52  GATC                 ^GATC
MboII         316  GAAGA                GAAGA(8/7)
MvaI          291  CCTGG                CC^[AT]GG
NdeII          52  GATC                 ^GATC
NlaIII        351  CATG                 CATG^
NspI          350  ACATGC               [GA]CATG^[CT]
PleI           46  GAGTC                GAGTC(4/5)
Sau3AI         52  GATC                 ^GATC
ScrFI         291  CCTGG                CC^[ACGT]GG
SfcI          274  CTACAG               C^T[GA][CT]AG
TfiI          106  GATTC                G^A[AT]TC
Tsp45I        300  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BsrI          127  CCAGT                ACTGG(1/-1)
              196  CCAGT               
Fnu4HI        227  GCCGC                GC^[ACGT]GC
              328  GCGGC               
HinfI          46  GAGTC                G^A[ACGT]TC
              106  GATTC               
ItaI          227  GCCGC                GC^[ACGT]GC
              328  GCGGC               
MaeIII        130  GTTAC                ^GT[ACGT]AC
              300  GTCAC               
MnlI            4  GAGG                 CCTC(7/6)
               10  GAGG                
MseI          139  TTAA                 T^TAA
              191  TTAA                
TaqI           14  TCGA                 T^CGA
              165  TCGA                
Tru9I         139  TTAA                 T^TAA
              191  TTAA                
TspRI         152  CAGTG                CA[GC]TG[ACGT][ACGT]^
              305  CACTG               
TthHB8I        14  TCGA                 T^CGA
              165  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

AciI        AluI        Tsp509I