################################## # NO CUT ENZYMES ( 213 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AcsI 343 AAATTT [GA]^AATT[CT] AlwNI 197 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 343 AAATTT [GA]^AATT[CT] BpmI 292 CTGGAG CTGGAG(16/14) BsmAI 162 GTCTC GTCTC(1/5) BssKI 291 CCTGG ^CC[ACGT]GG BstF5I 100 CATCC GGATG(2/0) BstNI 291 CCTGG CC^[AT]GG Cac8I 184 GCCCGC GC[ACGT]^[ACGT]GC DdeI 44 CTGAG C^T[ACGT]AG DpnII 52 GATC ^GATC DraI 190 TTTAAA TTT^AAA EarI 316 GAAGAG CTCTTC(1/4) EcoRII 291 CCTGG ^CC[AT]GG EcoRV 167 GATATC GAT^ATC FauI 185 CCCGC CCCGC(4/6) FokI 100 CATCC GGATG(9/13) HphI 301 TCACC GGTGA(8/7) Ksp632I 316 GAAGAG CTCTTC(1/4) MboI 52 GATC ^GATC MboII 316 GAAGA GAAGA(8/7) MvaI 291 CCTGG CC^[AT]GG NdeII 52 GATC ^GATC NlaIII 351 CATG CATG^ NspI 350 ACATGC [GA]CATG^[CT] PleI 46 GAGTC GAGTC(4/5) Sau3AI 52 GATC ^GATC ScrFI 291 CCTGG CC^[ACGT]GG SfcI 274 CTACAG C^T[GA][CT]AG TfiI 106 GATTC G^A[AT]TC Tsp45I 300 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BsrI 127 CCAGT ACTGG(1/-1) 196 CCAGT Fnu4HI 227 GCCGC GC^[ACGT]GC 328 GCGGC HinfI 46 GAGTC G^A[ACGT]TC 106 GATTC ItaI 227 GCCGC GC^[ACGT]GC 328 GCGGC MaeIII 130 GTTAC ^GT[ACGT]AC 300 GTCAC MnlI 4 GAGG CCTC(7/6) 10 GAGG MseI 139 TTAA T^TAA 191 TTAA TaqI 14 TCGA T^CGA 165 TCGA Tru9I 139 TTAA T^TAA 191 TTAA TspRI 152 CAGTG CA[GC]TG[ACGT][ACGT]^ 305 CACTG TthHB8I 14 TCGA T^CGA 165 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### AciI AluI Tsp509I