Restriction map for
pFN21AE5749
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AvrII
BalI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII Eco47III EcoRI EcoRV
FbaI FseI HgaI HpaI KpnI
KspI MamI MluI MluNI MroI
MscI NaeI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 1347 GTCTAC GT^[AC][GT]AC
AccII 55 CGCG CG^CG
AcsI 152 AAATTC [GA]^AATT[CT]
AcyI 1631 GGCGCC G[GA]^CG[CT]C
AfaI 769 GTAC GT^AC
AflIII 1568 ACACGT A^C[GA][CT]GT
AocI 1948 CCTAAGG CC^T[ACGT]AGG
ApoI 152 AAATTC [GA]^AATT[CT]
AviII 605 TGCGCA TGC^GCA
BbeI 1631 GGCGCC GGCGC^C
BfaI 655 CTAG C^TAG
BsaHI 1631 GGCGCC G[GA]^CG[CT]C
BsgI 807 CTGCAC GTGCAG(16/14)
BsiEI 1447 CGGCCG CG[GA][CT]^CG
BsrBI 1638 CCGCTC CCGCTC(-3/-3)
BssSI 1847 CACGAG CACGAG(-5/-1)
BstUI 55 CGCG CG^CG
Bsu36I 1948 CCTAAGG CC^T[ACGT]AGG
EagI 1447 CGGCCG C^GGCCG
EclXI 1447 CGGCCG C^GGCCG
Eco52I 1447 CGGCCG C^GGCCG
Eco81I 1948 CCTAAGG CC^T[ACGT]AGG
EcoNI 1101 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 589 ATGCAT ATGCA^T
FspI 605 TGCGCA TGC^GCA
HaeII 1631 GGCGCC [GA]GCGC^[CT]
Hin1I 1631 GGCGCC G[GA]^CG[CT]C
HincII 543 GTCAAC GT[CT]^[GA]AC
HindII 543 GTCAAC GT[CT]^[GA]AC
HindIII 1942 AAGCTT A^AGCTT
KasI 1631 GGCGCC G^GCGCC
MaeI 655 CTAG C^TAG
MfeI 728 CAATTG C^AATTG
MseI 374 TTAA T^TAA
MslI 420 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 728 CAATTG C^AATTG
MvnI 55 CGCG CG^CG
NarI 1631 GGCGCC GG^CGCC
NcoI 419 CCATGG C^CATGG
NsiI 589 ATGCAT ATGCA^T
PaeR7I 236 CTCGAG C^TCGAG
PstI 1102 CTGCAG CTGCA^G
PvuII 185 CAGCTG CAG^CTG
RsaI 769 GTAC GT^AC
SapI 531 GCTCTTC GCTCTTC(1/4)
SbfI 1101 CCTGCAGG CCTGCA^GG
SexAI 902 ACCTGGT A^CC[AT]GGT
SfoI 1631 GGCGCC GGC^GCC
SmaI 275 CCCGGG CCC^GGG
Sse8387I 1101 CCTGCAGG CCTGCA^GG
StuI 1686 AGGCCT AGG^CCT
Tru9I 374 TTAA T^TAA
XhoI 236 CTCGAG C^TCGAG
XmaCI 275 CCCGGG C^CCGGG
XmaI 275 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AhdI 38 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1311 GACAGGCGGTC
ApaI 1702 GGGCCC GGGCC^C
1953 GGGCCC
AspEI 38 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1311 GACAGGCGGTC
BamHI 1413 GGATCC G^GATCC
1536 GGATCC
BsaI 802 GAGACC GGTCTC(1/5)
1811 GAGACC
BsrDI 632 GCAATG GCAATG(2/0)
1064 GCAATG
BstEII 414 GGTTACC G^GT[ACGT]ACC
777 GGTGACC
BstPI 414 GGTTACC G^GT[ACGT]ACC
777 GGTGACC
BstXI 785 CCATTGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
896 CCAGGAACCTGG
DrdI 1666 GACAGGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1742 GACCTCAACGTC
Eam1105I 38 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1311 GACAGGCGGTC
EcoO65I 414 GGTTACC G^GT[ACGT]ACC
777 GGTGACC
EcoT14I 419 CCATGG C^C[AT][AT]GG
1985 CCTTGG
MspA1I 185 CAGCTG C[AC]G^C[GT]G
1200 CAGCGG
PpuMI 710 AGGACCT [GA]G^G[AT]CC[CT]
1470 GGGACCC
SmlI 236 CTCGAG C^T[CT][GA]AG
865 CTTGAG
StyI 419 CCATGG C^C[AT][AT]GG
1985 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AspI AvaI AvaII BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflFI PleI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII