Restriction map for
pFN21AE4210
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BseAI BsmI BspDI BspEI BspLU11I
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoRV
EcoT22I FbaI FseI FspI HaeII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 2026 GTCTAC GT^[AC][GT]AC
AcyI 1033 GACGCC G[GA]^CG[CT]C
Asp700I 1863 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1399 GAGCTC G[AT]GC[AT]^C
BamHI 623 GGATCC G^GATCC
BbsI 606 GAAGAC GAAGAC(2/6)
BcgI 890 CGATCACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2160 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2160 GCTCAGC GC^T[ACGT]AGC
BpuAI 606 GAAGAC GAAGAC(2/6)
BsaHI 1033 GACGCC G[GA]^CG[CT]C
BsiHKAI 1399 GAGCTC G[AT]GC[AT]^C
BsiWI 1506 CGTACG C^GTACG
BsmBI 1031 GAGACG CGTCTC(1/5)
Bsp1407I 965 TGTACA T^GTACA
BsrGI 965 TGTACA T^GTACA
BstEII 1611 GGTCACC G^GT[ACGT]ACC
BstPI 1611 GGTCACC G^GT[ACGT]ACC
BstYI 623 GGATCC [GA]^GATC[CT]
CelII 2160 GCTCAGC GC^T[ACGT]AGC
DraIII 1745 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1611 GGTCACC G^GT[ACGT]ACC
EcoRI 1279 GAATTC G^AATTC
Hin1I 1033 GACGCC G[GA]^CG[CT]C
HincII 766 GTCAAC GT[CT]^[GA]AC
HindII 766 GTCAAC GT[CT]^[GA]AC
MflI 623 GGATCC [GA]^GATC[CT]
MslI 1152 CATCGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1143 ACATGC [GA]CATG^[CT]
PaeR7I 442 CTCGAG C^TCGAG
PvuII 2069 CAGCTG CAG^CTG
SacI 1399 GAGCTC GAGCT^C
SmlI 442 CTCGAG C^T[CT][GA]AG
SspBI 965 TGTACA T^GTACA
XhoI 442 CTCGAG C^TCGAG
XhoII 623 GGATCC [GA]^GATC[CT]
XmnI 1863 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AocI 304 CCTCAGG CC^T[ACGT]AGG
591 CCTCAGG
ApaI 1272 GGGCCC GGGCC^C
1434 GGGCCC
BalI 92 TGGCCA TGG^CCA
1079 TGGCCA
BanI 147 GGCACC G^G[CT][GA]CC
361 GGCACC
BbvCI 414 CCTCAGC CCTCAGC(-5/-2)
1578 CCTCAGC
BsaWI 470 ACCGGA [AT]^CCGG[AT]
1361 ACCGGA
BsgI 1708 CTGCAC GTGCAG(16/14)
1159 GTGCAG
BsiEI 124 CGGCCG CG[GA][CT]^CG
1890 CGGCCG
BspHI 1871 TCATGA T^CATGA
2087 TCATGA
BspMI 875 ACCTGC ACCTGC(4/8)
1161 GCAGGT
BsrFI 122 ACCGGC [GA]^CCGG[CT]
514 ACCGGC
Bsu36I 304 CCTCAGG CC^T[ACGT]AGG
591 CCTCAGG
Cfr10I 122 ACCGGC [GA]^CCGG[CT]
514 ACCGGC
EagI 124 CGGCCG C^GGCCG
1890 CGGCCG
EclXI 124 CGGCCG C^GGCCG
1890 CGGCCG
Eco52I 124 CGGCCG C^GGCCG
1890 CGGCCG
Eco81I 304 CCTCAGG CC^T[ACGT]AGG
591 CCTCAGG
HindIII 208 AAGCTT A^AGCTT
646 AAGCTT
MluNI 92 TGGCCA TGG^CCA
1079 TGGCCA
MscI 92 TGGCCA TGG^CCA
1079 TGGCCA
MseI 860 TTAA T^TAA
1490 TTAA
MspA1I 1581 CAGCGG C[AC]G^C[GT]G
2069 CAGCTG
PpuMI 228 GGGACCC [GA]G^G[AT]CC[CT]
344 AGGACCC
PstI 898 CTGCAG CTGCA^G
1940 CTGCAG
RcaI 1871 TCATGA T^CATGA
2087 TCATGA
SapI 214 GAAGAGC GCTCTTC(1/4)
1695 GCTCTTC
StuI 728 AGGCCT AGG^CCT
924 AGGCCT
TfiI 704 GATTC G^A[AT]TC
958 GAATC
Tru9I 860 TTAA T^TAA
1490 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI