Restriction map for
pFN21AE2227
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BalI BamHI BanII BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HindIII KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TfiI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcyI 46 GGCGCC G[GA]^CG[CT]C
Asp700I 571 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 450 CCCGAG C^[CT]CG[GA]G
BbeI 46 GGCGCC GGCGC^C
BcnI 111 CCGGG CC^[GC]GG
BsaHI 46 GGCGCC G[GA]^CG[CT]C
BsoBI 450 CCCGAG C^[CT]CG[GA]G
BsrDI 683 GCAATG GCAATG(2/0)
BstYI 340 GGATCT [GA]^GATC[CT]
DraII 27 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 115 GACTCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 27 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1158 ATGCAT ATGCA^T
HgaI 825 GACGC GACGC(5/10)
Hin1I 46 GGCGCC G[GA]^CG[CT]C
HincII 871 GTTAAC GT[CT]^[GA]AC
HindII 871 GTTAAC GT[CT]^[GA]AC
HpaI 871 GTTAAC GTT^AAC
HphI 268 TCACC GGTGA(8/7)
KasI 46 GGCGCC G^GCGCC
MflI 340 GGATCT [GA]^GATC[CT]
MspA1I 34 CCGCTG C[AC]G^C[GT]G
NarI 46 GGCGCC GG^CGCC
NciI 111 CCGGG CC^[GC]GG
NsiI 1158 ATGCAT ATGCA^T
NspI 641 ACATGC [GA]CATG^[CT]
PpuMI 27 GGGTCCT [GA]G^G[AT]CC[CT]
SapI 150 GCTCTTC GCTCTTC(1/4)
SfaNI 1002 GATGC GCATC(5/9)
SfoI 46 GGCGCC GGC^GCC
SmlI 1006 CTCAAG C^T[CT][GA]AG
SspI 780 AATATT AAT^ATT
XcmI 845 CCATTAAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 340 GGATCT [GA]^GATC[CT]
XmnI 571 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 739 GGTACC G^GTACC
1058 GGTACC
AccII 127 CGCG CG^CG
178 CGCG
AfaI 740 GTAC GT^AC
1059 GTAC
Asp718I 739 GGTACC G^GTACC
1058 GGTACC
AvaII 28 GGTCC G^G[AT]CC
165 GGACC
BglI 135 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
175 GCCCGCGAGGC
BsgI 196 CTGCAC GTGCAG(16/14)
301 GTGCAG
BsmAI 497 GAGAC GTCTC(1/5)
306 GTCTC
BstUI 127 CGCG CG^CG
178 CGCG
EaeI 328 TGGCCG [CT]^GGCC[GA]
378 TGGCCG
EarI 151 CTCTTC CTCTTC(1/4)
223 GAAGAG
HapII 111 CCGG C^CGG
331 CCGG
HpaII 111 CCGG C^CGG
331 CCGG
KpnI 739 GGTACC GGTAC^C
1058 GGTACC
Ksp632I 151 CTCTTC CTCTTC(1/4)
223 GAAGAG
MspI 111 CCGG C^CGG
331 CCGG
MvnI 127 CGCG CG^CG
178 CGCG
NlaIII 43 CATG CATG^
642 CATG
RsaI 740 GTAC GT^AC
1059 GTAC
TaqI 233 TCGA T^CGA
993 TCGA
Tsp45I 250 GTCAC ^GT[GC]AC
948 GTCAC
TthHB8I 233 TCGA T^CGA
993 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI BanI BbvI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HinfI
ItaI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIV
PleI Sau3AI Sau96I ScrFI Tru9I
TseI Tsp509I TspRI