Restriction map for
pFN21AE4447
##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspHI AspI AvrII BalI BamHI
BbeI BbrPI BciVI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FauI FseI HgaI HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 1188 GACGTC GACGT^C
AccI 604 GTCTAC GT^[AC][GT]AC
AcsI 568 AAATTT [GA]^AATT[CT]
AcyI 1188 GACGTC G[GA]^CG[CT]C
AhdI 1276 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 152 GGATC GGATC(4/5)
ApaI 337 GGGCCC GGGCC^C
ApoI 568 AAATTT [GA]^AATT[CT]
Asp700I 319 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1276 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 196 CCCGGG C^[CT]CG[GA]G
AviII 549 TGCGCA TGC^GCA
BanI 475 GGCACC G^G[CT][GA]CC
BbvCI 906 CCTCAGC CCTCAGC(-5/-2)
BcgI 1183 GCAGGGACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 32 TGATCA T^GATCA
BglII 380 AGATCT A^GATCT
BsaHI 1188 GACGTC G[GA]^CG[CT]C
BsaI 121 GAGACC GGTCTC(1/5)
BsoBI 196 CCCGGG C^[CT]CG[GA]G
BsrFI 1356 GCCGGT [GA]^CCGG[CT]
BstEII 879 GGTCACC G^GT[ACGT]ACC
BstPI 879 GGTCACC G^GT[ACGT]ACC
Cfr10I 1356 GCCGGT [GA]^CCGG[CT]
DraII 336 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 232 GACATCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1276 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1225 GAAGAG CTCTTC(1/4)
EcoO109I 336 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 879 GGTCACC G^GT[ACGT]ACC
FbaI 32 TGATCA T^GATCA
FspI 549 TGCGCA TGC^GCA
Hin1I 1188 GACGTC G[GA]^CG[CT]C
HincII 1116 GTCAAC GT[CT]^[GA]AC
HindII 1116 GTCAAC GT[CT]^[GA]AC
Ksp632I 1225 GAAGAG CTCTTC(1/4)
MseI 332 TTAA T^TAA
NcoI 956 CCATGG C^CATGG
PstI 509 CTGCAG CTGCA^G
SfcI 509 CTGCAG C^T[GA][CT]AG
SmaI 196 CCCGGG CCC^GGG
SmlI 529 CTTGAG C^T[CT][GA]AG
TaqI 1192 TCGA T^CGA
Tru9I 332 TTAA T^TAA
TthHB8I 1192 TCGA T^CGA
XmaCI 196 CCCGGG C^CCGGG
XmaI 196 CCCGGG C^CCGGG
XmnI 319 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 290 ACGTGT A^C[GA][CT]GT
395 ACACGT
BanII 163 GGGCTC G[GA]GC[CT]^C
337 GGGCCC
BbsI 1230 GAAGAC GAAGAC(2/6)
991 GTCTTC
BpmI 657 CTCCAG CTGGAG(16/14)
463 CTGGAG
BpuAI 1230 GAAGAC GAAGAC(2/6)
991 GTCTTC
BspHI 29 TCATGA T^CATGA
737 TCATGA
BstYI 152 GGATCT [GA]^GATC[CT]
380 AGATCT
EcoT14I 956 CCATGG C^C[AT][AT]GG
1067 CCTTGG
HinfI 850 GAATC G^A[ACGT]TC
1289 GAATC
MflI 152 GGATCT [GA]^GATC[CT]
380 AGATCT
PflMI 675 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
956 CCATGGACTGG
RcaI 29 TCATGA T^CATGA
737 TCATGA
StyI 956 CCATGG C^C[AT][AT]GG
1067 CCTTGG
TfiI 850 GAATC G^A[AT]TC
1289 GAATC
TspRI 110 CAGTG CA[GC]TG[ACGT][ACGT]^
699 CACTG
Van91I 675 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
956 CCATGGACTGG
XcmI 626 CCACCAAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
629 CCAAGTCGGTGGTGG
XhoII 152 GGATCT [GA]^GATC[CT]
380 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I
Tsp509I