Restriction map for
pFN21AE0904
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaBI BsaI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI Tru9I Tsp509I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 213 GTATAC GT^[AC][GT]AC
AccII 976 CGCG CG^CG
AfaI 923 GTAC GT^AC
AflIII 962 ACACGT A^C[GA][CT]GT
AlwI 268 GATCC GGATC(4/5)
AspI 702 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 850 TGCGCA TGC^GCA
BalI 665 TGGCCA TGG^CCA
BanI 179 GGCACC G^G[CT][GA]CC
BfaI 515 CTAG C^TAG
BglI 1025 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 636 GCTCAGC GC^T[ACGT]AGC
BpmI 286 CTGGAG CTGGAG(16/14)
Bpu1102I 636 GCTCAGC GC^T[ACGT]AGC
BsaAI 920 CACGTA [CT]AC^GT[GA]
BsaWI 753 ACCGGA [AT]^CCGG[AT]
BsmBI 246 GAGACG CGTCTC(1/5)
Bst1107I 213 GTATAC GTA^TAC
BstUI 976 CGCG CG^CG
BstYI 267 AGATCC [GA]^GATC[CT]
BstZ17I 213 GTATAC GTA^TAC
CelII 636 GCTCAGC GC^T[ACGT]AGC
DpnII 268 GATC ^GATC
DraII 739 AGGCCCC [GA]G^G[ACGT]CC[CT]
EagI 911 CGGCCG C^GGCCG
EclXI 911 CGGCCG C^GGCCG
Eco52I 911 CGGCCG C^GGCCG
EcoNI 315 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 739 AGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 227 CCCGC CCCGC(4/6)
FspI 850 TGCGCA TGC^GCA
HincII 966 GTCAAC GT[CT]^[GA]AC
HindII 966 GTCAAC GT[CT]^[GA]AC
KspI 975 CCGCGG CCGC^GG
MaeI 515 CTAG C^TAG
MboI 268 GATC ^GATC
MflI 267 AGATCC [GA]^GATC[CT]
MluNI 665 TGGCCA TGG^CCA
MscI 665 TGGCCA TGG^CCA
MvnI 976 CGCG CG^CG
NdeII 268 GATC ^GATC
NlaIII 810 CATG CATG^
NotI 910 GCGGCCGC GC^GGCCGC
PflFI 702 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 536 CTGCAG CTGCA^G
PvuII 208 CAGCTG CAG^CTG
RsaI 923 GTAC GT^AC
SacII 975 CCGCGG CCGC^GG
Sau3AI 268 GATC ^GATC
SfcI 536 CTGCAG C^T[GA][CT]AG
SmaI 201 CCCGGG CCC^GGG
SmlI 689 CTCAAG C^T[CT][GA]AG
StuI 192 AGGCCT AGG^CCT
TaqI 1017 TCGA T^CGA
Tth111I 702 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1017 TCGA T^CGA
XhoII 267 AGATCC [GA]^GATC[CT]
XmaCI 201 CCCGGG C^CCGGG
XmaI 201 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 559 GACGCC G[GA]^CG[CT]C
1022 GACGCC
Aor51HI 30 AGCGCT AGC^GCT
614 AGCGCT
AspHI 64 GAGCAC G[AT]GC[AT]^C
342 GAGCAC
AvaI 34 CTCGGG C^[CT]CG[GA]G
201 CCCGGG
BbsI 574 GAAGAC GAAGAC(2/6)
113 GTCTTC
BpuAI 574 GAAGAC GAAGAC(2/6)
113 GTCTTC
BsaHI 559 GACGCC G[GA]^CG[CT]C
1022 GACGCC
BsgI 253 CTGCAC GTGCAG(16/14)
95 GTGCAG
BsiEI 628 CGACCG CG[GA][CT]^CG
911 CGGCCG
BsiHKAI 64 GAGCAC G[AT]GC[AT]^C
342 GAGCAC
BsmAI 246 GAGAC GTCTC(1/5)
700 GAGAC
BsmFI 38 GGGAC GGGAC(10/14)
708 GTCCC
BsoBI 34 CTCGGG C^[CT]CG[GA]G
201 CCCGGG
BsrI 136 ACTGG ACTGG(1/-1)
42 CCAGT
Eco47III 30 AGCGCT AGC^GCT
614 AGCGCT
HaeII 30 AGCGCT [GA]GCGC^[CT]
614 AGCGCT
Hin1I 559 GACGCC G[GA]^CG[CT]C
1022 GACGCC
HinfI 127 GACTC G^A[ACGT]TC
702 GACTC
HphI 332 TCACC GGTGA(8/7)
660 TCACC
PleI 127 GACTC GAGTC(4/5)
702 GACTC
SapI 835 GAAGAGC GCTCTTC(1/4)
1000 GAAGAGC
SfaNI 394 GATGC GCATC(5/9)
413 GCATC
TspRI 67 CACTG CA[GC]TG[ACGT][ACGT]^
1029 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DsaI
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI Ksp632I MaeII MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NlaIV Sau96I
ScrFI TseI Tsp45I