Restriction map for
pFN21AE3331
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII AccI AccII AclI AcyI
AflII AflIII AgeI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
AspI AvaI AviII BamHI BanII
BbeI BbrPI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaHI BsaI BsiEI
BsiWI BsmAI BsmBI BsoBI BspDI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KspI
MflI MluI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspI StuI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 362 GGTACC G^GTACC
AccIII 14 TCCGGA T^CCGGA
AciI 2186 GCGG CCGC(-3/-1)
AhdI 114 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 71 GGATC GGATC(4/5)
Asp718I 362 GGTACC G^GTACC
AspEI 114 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 357 CCTAGG C^CTAGG
BalI 2 TGGCCA TGG^CCA
BanI 362 GGTACC G^G[CT][GA]CC
BcgI 156 GCAGAAGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 357 CCTAGG C^CTAGG
BpmI 1988 CTGGAG CTGGAG(16/14)
BsaBI 487 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 14 TCCGGA [AT]^CCGG[AT]
BseAI 14 TCCGGA T^CCGGA
BseRI 277 GAGGAG GAGGAG(10/8)
BspEI 14 TCCGGA T^CCGGA
BsrI 1987 ACTGG ACTGG(1/-1)
BstXI 1674 CCATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 2 TGGCCA [CT]^GGCC[GA]
Eam1105I 114 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 382 GATATC GAT^ATC
EcoT22I 2809 ATGCAT ATGCA^T
FauI 2186 GCGGG CCCGC(4/6)
HapII 15 CCGG C^CGG
HincII 2497 GTCAAC GT[CT]^[GA]AC
HindII 2497 GTCAAC GT[CT]^[GA]AC
HpaII 15 CCGG C^CGG
KpnI 362 GGTACC GGTAC^C
MaeII 1948 ACGT A^CGT
MamI 487 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1593 CAATTG C^AATTG
MluNI 2 TGGCCA TGG^CCA
MroI 14 TCCGGA T^CCGGA
MscI 2 TGGCCA TGG^CCA
MspI 15 CCGG C^CGG
MunI 1593 CAATTG C^AATTG
NsiI 2809 ATGCAT ATGCA^T
PflMI 224 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 236 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 409 GAAGAGC GCTCTTC(1/4)
SexAI 347 ACCTGGT A^CC[AT]GGT
SpeI 646 ACTAGT A^CTAGT
Van91I 224 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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BbsI 563 GTCTTC GAAGAC(2/6)
1102 GTCTTC
BpuAI 563 GTCTTC GAAGAC(2/6)
1102 GTCTTC
BstAPI 1001 GCAGTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2177 GCAGGGAATGC
Cac8I 426 GCAGGC GC[ACGT]^[ACGT]GC
611 GCACGC
DdeI 568 CTTAG C^T[ACGT]AG
2719 CTCAG
DpnII 72 GATC ^GATC
478 GATC
DraII 32 GGGTCCC [GA]G^G[ACGT]CC[CT]
234 GGGACCT
EcoNI 2279 CCTTTAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2615 CCTTCATTAGG
EcoO109I 32 GGGTCCC [GA]G^G[ACGT]CC[CT]
234 GGGACCT
MboI 72 GATC ^GATC
478 GATC
MspA1I 286 CAGCTG C[AC]G^C[GT]G
834 CAGCTG
NdeII 72 GATC ^GATC
478 GATC
PpuMI 32 GGGTCCC [GA]G^G[AT]CC[CT]
234 GGGACCT
PvuII 286 CAGCTG CAG^CTG
834 CAGCTG
Sau3AI 72 GATC ^GATC
478 GATC
SfcI 339 CTATAG C^T[GA][CT]AG
1917 CTATAG
XcmI 108 CCAAGGGACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
454 CCAAACACAGTCTGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AcsI AfaI AluI Alw44I ApaLI
ApoI Asp700I AspHI AvaII BbvI
BfaI BmyI BsaJI BsgI BsiHKAI
BsiYI BslI BsmFI BsmI Bsp1286I
Bsp1407I BsrDI BsrGI BssKI BstF5I
BstNI Cfr13I DraI EarI EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboII MnlI MseI
MslI MvaI MwoI NdeI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
SfaNI SspBI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI