##################################
# NO CUT ENZYMES (  200 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BamHI       
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MfeI        MluI        MroI        MslI        
MspI        MunI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScrFI       SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          158  GTAGAC               GT^[AC][GT]AC
AciI           26  GCGG                 CCGC(-3/-1)
AluI          231  AGCT                 AG^CT
BalI          544  TGGCCA               TGG^CCA
BanI          463  GGTGCC               G^G[CT][GA]CC
BbsI          324  GAAGAC               GAAGAC(2/6)
BbvI            4  GCTGC                GCAGC(8/12)
BglII         573  AGATCT               A^GATCT
BpuAI         324  GAAGAC               GAAGAC(2/6)
BsaI           44  GAGACC               GGTCTC(1/5)
BsaJI          49  CCAAGG               C^C[ACGT][ACGT]GG
BseRI         271  GAGGAG               GAGGAG(10/8)
BsmAI          44  GAGAC                GTCTC(1/5)
BstF5I        483  GGATG                GGATG(2/0)
BstYI         573  AGATCT               [GA]^GATC[CT]
DdeI          468  CTCAG                C^T[ACGT]AG
EaeI          544  TGGCCA               [CT]^GGCC[GA]
EcoT14I        49  CCAAGG               C^C[AT][AT]GG
FauI           26  GCGGG                CCCGC(4/6)
Fnu4HI          4  GCTGC                GC^[ACGT]GC
FokI          483  GGATG                GGATG(9/13)
HphI          217  TCACC                GGTGA(8/7)
ItaI            4  GCTGC                GC^[ACGT]GC
MflI          573  AGATCT               [GA]^GATC[CT]
MluNI         544  TGGCCA               TGG^CCA
MscI          544  TGGCCA               TGG^CCA
MseI          422  TTAA                 T^TAA
MspA1I         24  CAGCGG               C[AC]G^C[GT]G
NlaIV         463  GGTGCC               GG[ACGT]^[ACGT]CC
ScaI          125  AGTACT               AGT^ACT
StyI           49  CCAAGG               C^C[AT][AT]GG
TfiI           19  GAATC                G^A[AT]TC
Tru9I         422  TTAA                 T^TAA
TseI            4  GCTGC                G^C[AT]GC
Tsp45I        382  GTGAC                ^GT[GC]AC
TspRI          57  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         573  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI          126  GTAC                 GT^AC
              331  GTAC                
DpnII         418  GATC                 ^GATC
              574  GATC                
EarI          268  GAAGAG               CTCTTC(1/4)
              288  GAAGAG              
HaeIII        397  GGCC                 GG^CC
              545  GGCC                
Ksp632I       268  GAAGAG               CTCTTC(1/4)
              288  GAAGAG              
MboI          418  GATC                 ^GATC
              574  GATC                
NdeII         418  GATC                 ^GATC
              574  GATC                
PleI           89  GAGTC                GAGTC(4/5)
              104  GAGTC               
RsaI          126  GTAC                 GT^AC
              331  GTAC                
Sau3AI        418  GATC                 ^GATC
              574  GATC                
SfaNI          70  GATGC                GCATC(5/9)
              566  GCATC               
SfcI          206  CTGTAG               C^T[GA][CT]AG
              347  CTATAG              
TaqI           87  TCGA                 T^CGA
              569  TCGA                
TthHB8I        87  TCGA                 T^CGA
              569  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

AcsI        ApoI        HinfI       MaeIII      MboII       
MnlI        Tsp509I