Restriction map for
pFN21AE5678
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AvaI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfrI BglI BpuAI BsaAI
BsaBI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 999 CGCG CG^CG
AcsI 152 AAATTC [GA]^AATT[CT]
AcyI 639 GACGCC G[GA]^CG[CT]C
AhdI 667 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1029 GGATC GGATC(4/5)
ApaI 284 GGGCCC GGGCC^C
ApoI 152 AAATTC [GA]^AATT[CT]
AspEI 667 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1114 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 432 TGCGCA TGC^GCA
BanI 1099 GGCACC G^G[CT][GA]CC
BanII 284 GGGCCC G[GA]GC[CT]^C
BbvI 939 GCTGC GCAGC(8/12)
BciVI 709 GGATAC GTATCC(6/5)
BcnI 1272 CCGGG CC^[GC]GG
BglII 815 AGATCT A^GATCT
BlpI 949 GCTCAGC GC^T[ACGT]AGC
BmyI 284 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 949 GCTCAGC GC^T[ACGT]AGC
BsaHI 639 GACGCC G[GA]^CG[CT]C
BsaI 440 GAGACC GGTCTC(1/5)
BsaWI 864 ACCGGA [AT]^CCGG[AT]
BsmI 1276 GAATGC GAATGC(1/-1)
Bsp1286I 284 GGGCCC G[AGT]GC[ACT]^C
BspHI 773 TCATGA T^CATGA
BspMI 1374 GCAGGT ACCTGC(4/8)
BsrDI 429 CATTGC GCAATG(2/0)
BsrFI 350 ACCGGC [GA]^CCGG[CT]
BstAPI 970 GCAGTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 483 GGATG GGATG(2/0)
BstUI 999 CGCG CG^CG
BstXI 1134 CCATGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 949 GCTCAGC GC^T[ACGT]AGC
Cfr10I 350 ACCGGC [GA]^CCGG[CT]
DraIII 1057 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 667 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI 483 GGATG GGATG(9/13)
FspI 432 TGCGCA TGC^GCA
HgaI 639 GACGC GACGC(5/10)
Hin1I 639 GACGCC G[GA]^CG[CT]C
MfeI 66 CAATTG C^AATTG
MunI 66 CAATTG C^AATTG
MvnI 999 CGCG CG^CG
NciI 1272 CCGGG CC^[GC]GG
PflFI 1114 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 576 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1300 CTGCAG CTGCA^G
RcaI 773 TCATGA T^CATGA
SapI 1384 GAAGAGC GCTCTTC(1/4)
SfcI 1300 CTGCAG C^T[GA][CT]AG
TseI 939 GCTGC G^C[AT]GC
Tth111I 1114 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 576 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 187 GTAC GT^AC
580 GTAC
AvrII 280 CCTAGG C^CTAGG
678 CCTAGG
BalI 827 TGGCCA TGG^CCA
1010 TGGCCA
BlnI 280 CCTAGG C^CTAGG
678 CCTAGG
BpmI 583 CTGGAG CTGGAG(16/14)
1214 CTGGAG
BseRI 7 GAGGAG GAGGAG(10/8)
868 GAGGAG
BsgI 193 CTGCAC GTGCAG(16/14)
1054 CTGCAC
BsmAI 440 GAGAC GTCTC(1/5)
873 GAGAC
BsmFI 675 GTCCC GGGAC(10/14)
1398 GTCCC
BstYI 815 AGATCT [GA]^GATC[CT]
1029 GGATCT
DraII 283 AGGGCCC [GA]G^G[ACGT]CC[CT]
923 AGGCCCT
EaeI 827 TGGCCA [CT]^GGCC[GA]
1010 TGGCCA
EcoO109I 283 AGGGCCC [GA]G^G[ACGT]CC[CT]
923 AGGCCCT
Fnu4HI 408 GCGGC GC^[ACGT]GC
939 GCTGC
ItaI 408 GCGGC GC^[ACGT]GC
939 GCTGC
MaeII 189 ACGT A^CGT
1396 ACGT
MflI 815 AGATCT [GA]^GATC[CT]
1029 GGATCT
MluNI 827 TGGCCA TGG^CCA
1010 TGGCCA
MscI 827 TGGCCA TGG^CCA
1010 TGGCCA
MslI 1129 CACGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1135 CATGAGAATG
MspA1I 952 CAGCTG C[AC]G^C[GT]G
1311 CAGCTG
MwoI 591 GCAAAGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
970 GCAGTGGTTGC
PvuII 952 CAGCTG CAG^CTG
1311 CAGCTG
RsaI 187 GTAC GT^AC
580 GTAC
SmlI 131 CTCAAG C^T[CT][GA]AG
445 CTCAAG
TaqI 884 TCGA T^CGA
1347 TCGA
TthHB8I 884 TCGA T^CGA
1347 TCGA
XhoII 815 AGATCT [GA]^GATC[CT]
1029 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AlwNI AvaII BfaI
BsaJI BsiYI BslI BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MspI MvaI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
Tru9I Tsp45I Tsp509I TspRI XcmI