Restriction map for
pFN21AE6358
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 336 CCGC CCGC(-3/-1)
AfaI 18 GTAC GT^AC
AflIII 416 ACGTGT A^C[GA][CT]GT
AhdI 181 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 535 GTGCAC G^TGCAC
AlwNI 550 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 535 GTGCAC G^TGCAC
AspEI 181 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 535 GTGCAC G[AT]GC[AT]^C
AvaII 363 GGTCC G^G[AT]CC
BfaI 715 CTAG C^TAG
BglII 657 AGATCT A^GATCT
BsaI 814 GAGACC GGTCTC(1/5)
BseRI 174 CTCCTC GAGGAG(10/8)
BsiHKAI 535 GTGCAC G[AT]GC[AT]^C
BsmAI 814 GAGAC GTCTC(1/5)
BspHI 53 TCATGA T^CATGA
BspMI 738 ACCTGC ACCTGC(4/8)
BstAPI 528 GCAAGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 528 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 489 GCGC GCG^C
DraIII 94 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 683 CCATGG C^C[GA][CT]GG
Eam1105I 181 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 618 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 488 GGCGCT [GA]GCGC^[CT]
HapII 811 CCGG C^CGG
HgaI 793 GACGC GACGC(5/10)
HhaI 489 GCGC GCG^C
HinP1I 489 GCGC G^CGC
HpaII 811 CCGG C^CGG
MaeI 715 CTAG C^TAG
MaeII 416 ACGT A^CGT
MspI 811 CCGG C^CGG
NcoI 683 CCATGG C^CATGG
NdeI 113 CATATG CA^TATG
NspI 32 ACATGC [GA]CATG^[CT]
PpuMI 362 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 126 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 445 CTGCAG CTGCA^G
RcaI 53 TCATGA T^CATGA
RsaI 18 GTAC GT^AC
SmlI 970 CTTGAG C^T[CT][GA]AG
TaqI 381 TCGA T^CGA
TfiI 160 GAATC G^A[AT]TC
Tsp509I 197 AATT ^AATT
TthHB8I 381 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwI 768 GATCC GGATC(4/5)
952 GATCC
BanII 314 GGGCTC G[GA]GC[CT]^C
385 GGGCTC
BsmI 276 GAATGC GAATGC(1/-1)
351 GAATGC
BstXI 248 CCAAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
653 CCATAGATCTGG
BstYI 657 AGATCT [GA]^GATC[CT]
951 AGATCC
DraII 362 AGGTCCT [GA]G^G[ACGT]CC[CT]
702 GGGGCCT
EcoO109I 362 AGGTCCT [GA]G^G[ACGT]CC[CT]
702 GGGGCCT
MaeIII 213 GTCAC ^GT[ACGT]AC
854 GTGAC
MboII 862 GAAGA GAAGA(8/7)
378 TCTTC
MflI 657 AGATCT [GA]^GATC[CT]
951 AGATCC
MseI 734 TTAA T^TAA
745 TTAA
MspA1I 448 CAGCTG C[AC]G^C[GT]G
550 CAGCTG
MwoI 528 GCAAGCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
718 GCGATCAGTGC
PleI 47 GACTC GAGTC(4/5)
966 GAGTC
PvuII 448 CAGCTG CAG^CTG
550 CAGCTG
Tru9I 734 TTAA T^TAA
745 TTAA
Tsp45I 213 GTCAC ^GT[GC]AC
854 GTGAC
XhoII 657 AGATCT [GA]^GATC[CT]
951 AGATCC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI BbvI BmyI BpmI BsaJI
BsgI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI ItaI MboI MnlI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI TspRI