Restriction map for
pFN21AE6743
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AclI AflIII
AgeI AhdI AocI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BclI BfaI
BglII BlnI BsaBI BsgI BsiWI
BsmBI BsmFI Bsp1407I BspDI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco81I EcoNI EcoO65I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI HphI KpnI Ksp632I
KspI MaeI MamI MfeI MflI
MluI MluNI MscI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 1436 CGCG CG^CG
AccIII 134 TCCGGA T^CCGGA
AflII 1002 CTTAAG C^TTAAG
Alw44I 1124 GTGCAC G^TGCAC
AlwI 1272 GATCC GGATC(4/5)
Aor51HI 1871 AGCGCT AGC^GCT
ApaLI 1124 GTGCAC G^TGCAC
BanII 1258 GAGCTC G[GA]GC[CT]^C
BbsI 850 GAAGAC GAAGAC(2/6)
BbvCI 1165 GCTGAGG CCTCAGC(-5/-2)
BfrI 1002 CTTAAG C^TTAAG
BlpI 1021 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1021 GCTGAGC GC^T[ACGT]AGC
BpuAI 850 GAAGAC GAAGAC(2/6)
BsaAI 1445 TACGTG [CT]AC^GT[GA]
BsaWI 134 TCCGGA [AT]^CCGG[AT]
BseAI 134 TCCGGA T^CCGGA
BsiEI 51 CGGCCG CG[GA][CT]^CG
BsmI 1314 GAATGC GAATGC(1/-1)
BspEI 134 TCCGGA T^CCGGA
BstUI 1436 CGCG CG^CG
BstXI 200 CCATCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1021 GCTGAGC GC^T[ACGT]AGC
EagI 51 CGGCCG C^GGCCG
EclXI 51 CGGCCG C^GGCCG
Eco47III 1871 AGCGCT AGC^GCT
Eco52I 51 CGGCCG C^GGCCG
EcoRI 506 GAATTC G^AATTC
EcoRV 1138 GATATC GAT^ATC
EcoT14I 1073 CCATGG C^C[AT][AT]GG
HgaI 1018 GACGC GACGC(5/10)
MroI 134 TCCGGA T^CCGGA
MseI 1003 TTAA T^TAA
MvnI 1436 CGCG CG^CG
NcoI 1073 CCATGG C^CATGG
SacI 1258 GAGCTC GAGCT^C
SexAI 842 ACCTGGT A^CC[AT]GGT
SmaI 224 CCCGGG CCC^GGG
StyI 1073 CCATGG C^C[AT][AT]GG
Tru9I 1003 TTAA T^TAA
XmaCI 224 CCCGGG C^CCGGG
XmaI 224 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 506 GAATTC [GA]^AATT[CT]
907 GAATTT
AcyI 809 GGCGCC G[GA]^CG[CT]C
1780 GGCGCC
ApoI 506 GAATTC [GA]^AATT[CT]
907 GAATTT
AspHI 1124 GTGCAC G[AT]GC[AT]^C
1258 GAGCTC
AvaI 224 CCCGGG C^[CT]CG[GA]G
796 CTCGGG
BanI 809 GGCGCC G^G[CT][GA]CC
1780 GGCGCC
BbeI 809 GGCGCC GGCGC^C
1780 GGCGCC
BglI 485 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1694 GCCGGGGAGGC
BsaHI 809 GGCGCC G[GA]^CG[CT]C
1780 GGCGCC
BsaI 679 GAGACC GGTCTC(1/5)
66 GGTCTC
BseRI 1525 GAGGAG GAGGAG(10/8)
1616 GAGGAG
BsiHKAI 1124 GTGCAC G[AT]GC[AT]^C
1258 GAGCTC
BsoBI 224 CCCGGG C^[CT]CG[GA]G
796 CTCGGG
BsrDI 1110 CATTGC GCAATG(2/0)
1335 CATTGC
BsrI 1851 CCAGT ACTGG(1/-1)
1860 CCAGT
Hin1I 809 GGCGCC G[GA]^CG[CT]C
1780 GGCGCC
KasI 809 GGCGCC G^GCGCC
1780 GGCGCC
NarI 809 GGCGCC GG^CGCC
1780 GGCGCC
PpuMI 839 AGGACCT [GA]G^G[AT]CC[CT]
1006 AGGACCC
SfoI 809 GGCGCC GGC^GCC
1780 GGCGCC
TfiI 756 GAATC G^A[AT]TC
1712 GAATC
Tsp509I 507 AATT ^AATT
908 AATT
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrBI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TseI Tsp45I TspRI TthHB8I