Restriction map for
pFN21AE2318
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# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccI AccIII AclI Aor51HI
AscI AseI AsnI AviII BbrPI
BsaAI BseAI BsiEI BsiWI BspEI
BssHII Bst1107I BstEII BstPI BstZ17I
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI FseI
FspI KspI MluI MroI NaeI
NgoMIV NheI NotI NruI PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SnaBI Sse8387I
SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 3285 GGTACC G^GTACC
AgeI 3145 ACCGGT A^CCGGT
AhdI 3244 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1817 GGGCCC GGGCC^C
Asp718I 3285 GGTACC G^GTACC
AspEI 3244 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3706 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 636 CCTAGG C^CTAGG
BbvCI 5108 CCTCAGC CCTCAGC(-5/-2)
BglI 1075 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 636 CCTAGG C^CTAGG
BsmBI 1984 CGTCTC CGTCTC(1/5)
BsmI 3951 GAATGC GAATGC(1/-1)
BspDI 5044 ATCGAT AT^CGAT
BspMI 3568 ACCTGC ACCTGC(4/8)
BsrFI 3145 ACCGGT [GA]^CCGG[CT]
BssSI 5032 CACGAG CACGAG(-5/-1)
BstBI 830 TTCGAA TT^CGAA
Cfr10I 3145 ACCGGT [GA]^CCGG[CT]
ClaI 5044 ATCGAT AT^CGAT
DraI 1294 TTTAAA TTT^AAA
Eam1105I 3244 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3412 GATATC GAT^ATC
EcoT22I 2755 ATGCAT ATGCA^T
HindIII 4132 AAGCTT A^AGCTT
HpaI 4036 GTTAAC GTT^AAC
KpnI 3285 GGTACC GGTAC^C
NsiI 2755 ATGCAT ATGCA^T
NspV 830 TTCGAA TT^CGAA
PflFI 3706 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3145 ACCGGT A^CCGGT
SexAI 4673 ACCTGGT A^CC[AT]GGT
SfuI 830 TTCGAA TT^CGAA
SgrAI 3144 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1728 CCCGGG CCC^GGG
SphI 950 GCATGC GCATG^C
Tth111I 3706 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1728 CCCGGG C^CCGGG
XmaI 1728 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflII 1315 CTTAAG C^TTAAG
3909 CTTAAG
Alw44I 417 GTGCAC G^TGCAC
3043 GTGCAC
AocI 3451 CCTGAGG CC^T[ACGT]AGG
3882 CCTTAGG
ApaLI 417 GTGCAC G^TGCAC
3043 GTGCAC
AvaI 1534 CCCGAG C^[CT]CG[GA]G
1728 CCCGGG
BcgI 2791 CGAGTGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5385 CGACCCCGATGC
BciVI 1006 GGATAC GTATCC(6/5)
4204 GGATAC
BclI 407 TGATCA T^GATCA
4889 TGATCA
BfrI 1315 CTTAAG C^TTAAG
3909 CTTAAG
BglII 2993 AGATCT A^GATCT
5369 AGATCT
BlpI 1641 GCTCAGC GC^T[ACGT]AGC
3627 GCTGAGC
Bpu1102I 1641 GCTCAGC GC^T[ACGT]AGC
3627 GCTGAGC
BsaWI 3145 ACCGGT [AT]^CCGG[AT]
3161 ACCGGA
BsoBI 1534 CCCGAG C^[CT]CG[GA]G
1728 CCCGGG
Bsu36I 3451 CCTGAGG CC^T[ACGT]AGG
3882 CCTTAGG
CelII 1641 GCTCAGC GC^T[ACGT]AGC
3627 GCTGAGC
Eco81I 3451 CCTGAGG CC^T[ACGT]AGG
3882 CCTTAGG
EcoNI 1989 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5166 CCTAGATAAGG
FbaI 407 TGATCA T^GATCA
4889 TGATCA
MfeI 716 CAATTG C^AATTG
1129 CAATTG
MunI 716 CAATTG C^AATTG
1129 CAATTG
NdeI 2752 CATATG CA^TATG
5021 CATATG
ScaI 4460 AGTACT AGT^ACT
5411 AGTACT
SpeI 519 ACTAGT A^CTAGT
2121 ACTAGT
XcmI 4472 CCATGAAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5555 CCAAGCGGGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BalI BamHI
BanI BanII BbeI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspHI BspLU11I
BsrBI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI