Restriction map for
pFN21AE2376
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BamHI
BbrPI BbvCI BciVI BclI BfrI
BlnI BsaAI BsaBI BsaWI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeII MamI
MfeI MluI MluNI MroI MscI
MunI NcoI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SalI SapI SbfI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tsp45I Van91I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 694 GTAGAC GT^[AC][GT]AC
AcyI 427 GGCGCC G[GA]^CG[CT]C
AflIII 855 ACATGT A^C[GA][CT]GT
Asp700I 1360 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 37 GAGCTC G[AT]GC[AT]^C
AspI 240 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 427 GGCGCC G^G[CT][GA]CC
BbeI 427 GGCGCC GGCGC^C
BcgI 1225 CGAGCCATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 828 AGATCT A^GATCT
BlpI 97 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 97 GCTGAGC GC^T[ACGT]AGC
BsaHI 427 GGCGCC G[GA]^CG[CT]C
BsaI 406 GAGACC GGTCTC(1/5)
BsiHKAI 37 GAGCTC G[AT]GC[AT]^C
BsmBI 238 GAGACG CGTCTC(1/5)
BspLU11I 855 ACATGT A^CATGT
BsrBI 411 CCGCTC CCGCTC(-3/-3)
BsrFI 605 GCCGGC [GA]^CCGG[CT]
BstYI 828 AGATCT [GA]^GATC[CT]
CelII 97 GCTGAGC GC^T[ACGT]AGC
Cfr10I 605 GCCGGC [GA]^CCGG[CT]
DraII 467 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 962 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 467 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 482 CCAAGG C^C[AT][AT]GG
HgaI 1163 GACGC GACGC(5/10)
Hin1I 427 GGCGCC G[GA]^CG[CT]C
KasI 427 GGCGCC G^GCGCC
KspI 490 CCGCGG CCGC^GG
MaeIII 942 GTTAC ^GT[ACGT]AC
MflI 828 AGATCT [GA]^GATC[CT]
NaeI 605 GCCGGC GCC^GGC
NarI 427 GGCGCC GG^CGCC
NgoMIV 605 GCCGGC G^CCGGC
NheI 1032 GCTAGC G^CTAGC
NspI 855 ACATGT [GA]CATG^[CT]
PflFI 240 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 658 GACTC GAGTC(4/5)
SacI 37 GAGCTC GAGCT^C
SacII 490 CCGCGG CCGC^GG
SfoI 427 GGCGCC GGC^GCC
SmaI 72 CCCGGG CCC^GGG
SspI 772 AATATT AAT^ATT
StyI 482 CCAAGG C^C[AT][AT]GG
Tth111I 240 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 828 AGATCT [GA]^GATC[CT]
XmaCI 72 CCCGGG C^CCGGG
XmaI 72 CCCGGG C^CCGGG
XmnI 1360 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
BbsI 289 GAAGAC GAAGAC(2/6)
1360 GAAGAC
BpuAI 289 GAAGAC GAAGAC(2/6)
1360 GAAGAC
BsrDI 730 GCAATG GCAATG(2/0)
1004 GCAATG
DsaI 490 CCGCGG C^C[GA][CT]GG
1174 CCACGG
MslI 15 CACCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
856 CATGTAGATG
NdeI 952 CATATG CA^TATG
1230 CATATG
ScaI 679 AGTACT AGT^ACT
1294 AGTACT
TaqI 1193 TCGA T^CGA
1224 TCGA
TfiI 1108 GAATC G^A[AT]TC
1321 GATTC
TthHB8I 1193 TCGA T^CGA
1224 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI Tru9I TseI Tsp509I TspRI