Restriction map for
pFN21AE6731
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# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AcsI AgeI Aor51HI
ApoI AscI AseI AsnI AspI
AvrII BbrPI BclI BlnI BsiWI
BspHI BssSI Bst1107I BstBI BstZ17I
CpoI DraI DraIII Eco47III EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI Tsp509I Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 1510 GGTACC G^GTACC
AflII 1606 CTTAAG C^TTAAG
AhdI 2158 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 385 GTGCAC G^TGCAC
ApaLI 385 GTGCAC G^TGCAC
Asp700I 36 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1510 GGTACC G^GTACC
AspEI 2158 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 1618 GAAGAC GAAGAC(2/6)
BcgI 2654 CGAGGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3568 GTATCC GTATCC(6/5)
BfrI 1606 CTTAAG C^TTAAG
BlpI 2763 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2763 GCTCAGC GC^T[ACGT]AGC
BpuAI 1618 GAAGAC GAAGAC(2/6)
BsaAI 1264 TACGTG [CT]AC^GT[GA]
BsaBI 639 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1926 CGTCTC CGTCTC(1/5)
Bsp1407I 1517 TGTACA T^GTACA
BspDI 2459 ATCGAT AT^CGAT
BspLU11I 4018 ACATGT A^CATGT
BsrGI 1517 TGTACA T^GTACA
BssHII 398 GCGCGC G^CGCGC
CelII 2763 GCTCAGC GC^T[ACGT]AGC
ClaI 2459 ATCGAT AT^CGAT
DrdI 409 GACGAAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2158 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KpnI 1510 GGTACC GGTAC^C
MamI 639 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 4030 GCCGGC GCC^GGC
NgoMIV 4030 GCCGGC G^CCGGC
PshAI 3446 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2761 GAGCTC GAGCT^C
SapI 3763 GCTCTTC GCTCTTC(1/4)
SspBI 1517 TGTACA T^GTACA
XmnI 36 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 418 GTCTAC GT^[AC][GT]AC
863 GTCTAC
AccIII 914 TCCGGA T^CCGGA
3746 TCCGGA
AflIII 1265 ACGTGT A^C[GA][CT]GT
4018 ACATGT
ApaI 3842 GGGCCC GGGCC^C
4077 GGGCCC
AviII 336 TGCGCA TGC^GCA
782 TGCGCA
BamHI 327 GGATCC G^GATCC
849 GGATCC
BbvCI 3642 CCTCAGC CCTCAGC(-5/-2)
3852 GCTGAGG
BseAI 914 TCCGGA T^CCGGA
3746 TCCGGA
BsmI 556 GAATGC GAATGC(1/-1)
2285 GCATTC
BspEI 914 TCCGGA T^CCGGA
3746 TCCGGA
BsrBI 1596 GAGCGG CCGCTC(-3/-3)
3883 GAGCGG
BsrDI 2435 GCAATG GCAATG(2/0)
2488 GCAATG
BsrFI 2796 ACCGGC [GA]^CCGG[CT]
4030 GCCGGC
BstAPI 339 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1464 GCAGTGGCTGC
BstEII 1047 GGTGACC G^GT[ACGT]ACC
3399 GGTCACC
BstPI 1047 GGTGACC G^GT[ACGT]ACC
3399 GGTCACC
Cfr10I 2796 ACCGGC [GA]^CCGG[CT]
4030 GCCGGC
EcoO65I 1047 GGTGACC G^GT[ACGT]ACC
3399 GGTCACC
FspI 336 TGCGCA TGC^GCA
782 TGCGCA
HindIII 3202 AAGCTT A^AGCTT
3987 AAGCTT
KspI 570 CCGCGG CCGC^GG
1235 CCGCGG
MroI 914 TCCGGA T^CCGGA
3746 TCCGGA
MseI 1226 TTAA T^TAA
1607 TTAA
SacII 570 CCGCGG CCGC^GG
1235 CCGCGG
SbfI 2022 CCTGCAGG CCTGCA^GG
2055 CCTGCAGG
ScaI 549 AGTACT AGT^ACT
2182 AGTACT
Sse8387I 2022 CCTGCAGG CCTGCA^GG
2055 CCTGCAGG
Tru9I 1226 TTAA T^TAA
1607 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI