Restriction map for
pFN21AE3293
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# NO CUT ENZYMES ( 113 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BclI BfrI
BglII BlnI BsaAI BsaWI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BsrFI Bst1107I
BstBI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MflI MluI MroI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI StyI SwaI TfiI Tth111I
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AatII 2155 GACGTC GACGT^C
AccI 1156 GTAGAC GT^[AC][GT]AC
BbsI 43 GTCTTC GAAGAC(2/6)
BbvCI 1474 GCTGAGG CCTCAGC(-5/-2)
BcgI 1224 CGACCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1183 GGATAC GTATCC(6/5)
BglI 2086 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 43 GTCTTC GAAGAC(2/6)
BsaBI 699 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1032 CGACCG CG[GA][CT]^CG
Bsp1407I 626 TGTACA T^GTACA
BsrGI 626 TGTACA T^GTACA
BssHII 1330 GCGCGC G^CGCGC
BssSI 1150 CACGAG CACGAG(-5/-1)
BstAPI 1246 GCACATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1086 GGTCACC G^GT[ACGT]ACC
BstPI 1086 GGTCACC G^GT[ACGT]ACC
EcoO65I 1086 GGTCACC G^GT[ACGT]ACC
EcoT22I 469 ATGCAT ATGCA^T
HgaI 175 GACGC GACGC(5/10)
MamI 699 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 638 CAATTG C^AATTG
MunI 638 CAATTG C^AATTG
NsiI 469 ATGCAT ATGCA^T
SmaI 949 CCCGGG CCC^GGG
SspBI 626 TGTACA T^GTACA
XbaI 834 TCTAGA T^CTAGA
XmaCI 949 CCCGGG C^CCGGG
XmaI 949 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcsI 479 AAATTT [GA]^AATT[CT]
1041 GAATTT
ApaI 1016 GGGCCC GGGCC^C
1286 GGGCCC
ApoI 479 AAATTT [GA]^AATT[CT]
1041 GAATTT
AspHI 1859 GTGCTC G[AT]GC[AT]^C
2007 GAGCAC
AvaI 949 CCCGGG C^[CT]CG[GA]G
1050 CCCGAG
BanI 74 GGCGCC G^G[CT][GA]CC
2143 GGCGCC
BanII 1016 GGGCCC G[GA]GC[CT]^C
1286 GGGCCC
BbeI 74 GGCGCC GGCGC^C
2143 GGCGCC
BlpI 561 GCTCAGC GC^T[ACGT]AGC
1564 GCTCAGC
Bpu1102I 561 GCTCAGC GC^T[ACGT]AGC
1564 GCTCAGC
BsaI 376 GAGACC GGTCTC(1/5)
1440 GGTCTC
BseRI 1912 GAGGAG GAGGAG(10/8)
2004 GAGGAG
BsiHKAI 1859 GTGCTC G[AT]GC[AT]^C
2007 GAGCAC
BsoBI 949 CCCGGG C^[CT]CG[GA]G
1050 CCCGAG
BstXI 500 CCAAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1262 CCAACCGAGTGG
CelII 561 GCTCAGC GC^T[ACGT]AGC
1564 GCTCAGC
DraIII 244 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
1247 CACATGGTG
DsaI 1347 CCACGG C^C[GA][CT]GG
1905 CCACGG
EcoNI 315 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1464 CCTGCATGAGG
KasI 74 GGCGCC G^GCGCC
2143 GGCGCC
MseI 410 TTAA T^TAA
959 TTAA
NarI 74 GGCGCC GG^CGCC
2143 GGCGCC
PpuMI 609 AGGACCT [GA]G^G[AT]CC[CT]
809 AGGACCC
PvuII 1700 CAGCTG CAG^CTG
2149 CAGCTG
SfoI 74 GGCGCC GGC^GCC
2143 GGCGCC
SphI 557 GCATGC GCATG^C
722 GCATGC
Tru9I 410 TTAA T^TAA
959 TTAA
XcmI 305 CCAACGGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1170 CCAGACGGCCTCTGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaII BalI BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I