Restriction map for
pFN21AE5775
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AocI Aor51HI ApaI AscI
AseI AsnI AspI AviII AvrII
BbvCI BcgI BfrI BlnI BsaBI
BsaI BsaWI BseAI BseRI BsiWI
BsmBI Bsp1407I BspEI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I Cfr10I CpoI DrdI Eco47III
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HpaI KspI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 74 enzymes) #
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Acc65I 817 GGTACC G^GTACC
AccII 163 CGCG CG^CG
AcyI 11 GGCGCC G[GA]^CG[CT]C
AflIII 479 ACGTGT A^C[GA][CT]GT
AhdI 847 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 519 GTGCAC G^TGCAC
ApaLI 519 GTGCAC G^TGCAC
Asp700I 934 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 817 GGTACC G^GTACC
AspEI 847 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1120 GGACC G^G[AT]CC
BalI 1020 TGGCCA TGG^CCA
BamHI 129 GGATCC G^GATCC
BbeI 11 GGCGCC GGCGC^C
BbrPI 364 CACGTG CAC^GTG
BclI 243 TGATCA T^GATCA
BcnI 111 CCCGG CC^[GC]GG
BglI 115 GCCGAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1031 AGATCT A^GATCT
BlpI 1296 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1296 GCTGAGC GC^T[ACGT]AGC
BsaAI 364 CACGTG [CT]AC^GT[GA]
BsaHI 11 GGCGCC G[GA]^CG[CT]C
BsiEI 113 CGGCCG CG[GA][CT]^CG
BsmI 1250 GCATTC GAATGC(1/-1)
BspDI 175 ATCGAT AT^CGAT
BspHI 1170 TCATGA T^CATGA
BspMI 97 ACCTGC ACCTGC(4/8)
BsrBI 53 CCGCTC CCGCTC(-3/-3)
BssSI 229 CACGAG CACGAG(-5/-1)
BstAPI 153 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 637 GGTAACC G^GT[ACGT]ACC
BstPI 637 GGTAACC G^GT[ACGT]ACC
BstUI 163 CGCG CG^CG
BstXI 133 CCACTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1296 GCTGAGC GC^T[ACGT]AGC
ClaI 175 ATCGAT AT^CGAT
DraI 1576 TTTAAA TTT^AAA
DraII 1790 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1666 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 446 CCATGG C^C[GA][CT]GG
EagI 113 CGGCCG C^GGCCG
Eam1105I 847 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 983 GAAGAG CTCTTC(1/4)
EclXI 113 CGGCCG C^GGCCG
Eco52I 113 CGGCCG C^GGCCG
EcoNI 595 CCTTTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1790 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 637 GGTAACC G^GT[ACGT]ACC
FbaI 243 TGATCA T^GATCA
HgaI 275 GACGC GACGC(5/10)
Hin1I 11 GGCGCC G[GA]^CG[CT]C
HindIII 1001 AAGCTT A^AGCTT
KasI 11 GGCGCC G^GCGCC
KpnI 817 GGTACC GGTAC^C
Ksp632I 983 GAAGAG CTCTTC(1/4)
MluNI 1020 TGGCCA TGG^CCA
MscI 1020 TGGCCA TGG^CCA
MvnI 163 CGCG CG^CG
NarI 11 GGCGCC GG^CGCC
NciI 111 CCCGG CC^[GC]GG
NcoI 446 CCATGG C^CATGG
NspI 1059 GCATGT [GA]CATG^[CT]
PmaCI 364 CACGTG CAC^GTG
PmlI 364 CACGTG CAC^GTG
PstI 151 CTGCAG CTGCA^G
RcaI 1170 TCATGA T^CATGA
SacI 1287 GAGCTC GAGCT^C
SfoI 11 GGCGCC GGC^GCC
SmlI 970 CTCAAG C^T[CT][GA]AG
SpeI 1423 ACTAGT A^CTAGT
XbaI 1254 TCTAGA T^CTAGA
XcmI 446 CCATGGCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 934 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 154 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1752 CAGTCTCTG
AvaI 125 CTCGGG C^[CT]CG[GA]G
1676 CCCGAG
BanI 11 GGCGCC G^G[CT][GA]CC
817 GGTACC
BanII 463 GGGCTC G[GA]GC[CT]^C
1287 GAGCTC
BciVI 483 GTATCC GTATCC(6/5)
1593 GTATCC
BpmI 103 CTCCAG CTGGAG(16/14)
1509 CTGGAG
BsoBI 125 CTCGGG C^[CT]CG[GA]G
1676 CCCGAG
BsrDI 747 CATTGC GCAATG(2/0)
1277 CATTGC
EaeI 113 CGGCCG [CT]^GGCC[GA]
1020 TGGCCA
EcoT14I 446 CCATGG C^C[AT][AT]GG
1188 CCTTGG
FauI 264 CCCGC CCCGC(4/6)
771 CCCGC
HaeII 11 GGCGCC [GA]GCGC^[CT]
107 AGCGCC
HapII 112 CCGG C^CGG
1282 CCGG
HpaII 112 CCGG C^CGG
1282 CCGG
HphI 581 TCACC GGTGA(8/7)
1796 TCACC
MslI 707 CACCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1852 CACCTATATG
MspI 112 CCGG C^CGG
1282 CCGG
SfaNI 1731 GCATC GCATC(5/9)
1813 GCATC
SfcI 151 CTGCAG C^T[GA][CT]AG
1761 CTATAG
StyI 446 CCATGG C^C[AT][AT]GG
1188 CCTTGG
TaqI 176 TCGA T^CGA
1076 TCGA
Tsp45I 845 GTGAC ^GT[GC]AC
1784 GTGAC
TthHB8I 176 TCGA T^CGA
1076 TCGA
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BbsI BbvI BfaI
BmyI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI TfiI Tru9I
TseI Tsp509I TspRI XhoII