Restriction map for
pFN21AE4061
##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpmI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI FauI
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeIII MamI MluI MluNI
MroI MscI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI Tsp45I Tth111I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 561 CGCG CG^CG
AlwNI 589 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 268 ATTAAT AT^TAAT
AsnI 268 ATTAAT AT^TAAT
Asp700I 3 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 761 GAGCTC G[AT]GC[AT]^C
BanII 761 GAGCTC G[GA]GC[CT]^C
BcnI 136 CCGGG CC^[GC]GG
BfaI 146 CTAG C^TAG
BlpI 583 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 583 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 761 GAGCTC G[AT]GC[AT]^C
BsmAI 810 GTCTC GTCTC(1/5)
Bsp1407I 542 TGTACA T^GTACA
BspMI 588 GCAGGT ACCTGC(4/8)
BsrGI 542 TGTACA T^GTACA
BstUI 561 CGCG CG^CG
BstYI 116 GGATCT [GA]^GATC[CT]
Cac8I 310 GCTTGC GC[ACGT]^[ACGT]GC
CelII 583 GCTCAGC GC^T[ACGT]AGC
DsaI 878 CCATGG C^C[GA][CT]GG
EcoT22I 841 ATGCAT ATGCA^T
HaeIII 375 GGCC GG^CC
HapII 136 CCGG C^CGG
HgaI 223 GACGC GACGC(5/10)
HpaII 136 CCGG C^CGG
MaeI 146 CTAG C^TAG
MaeII 278 ACGT A^CGT
MflI 116 GGATCT [GA]^GATC[CT]
MspI 136 CCGG C^CGG
MvnI 561 CGCG CG^CG
NciI 136 CCGGG CC^[GC]GG
NcoI 878 CCATGG C^CATGG
NsiI 841 ATGCAT ATGCA^T
PleI 601 GACTC GAGTC(4/5)
PshBI 268 ATTAAT AT^TAAT
PstI 940 CTGCAG CTGCA^G
SacI 761 GAGCTC GAGCT^C
SspBI 542 TGTACA T^GTACA
StuI 374 AGGCCT AGG^CCT
XbaI 145 TCTAGA T^CTAGA
XhoII 116 GGATCT [GA]^GATC[CT]
XmnI 3 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AvaII 591 GGTCC G^G[AT]CC
873 GGACC
BmyI 761 GAGCTC G[AGT]GC[ACT]^C
963 GTGCCC
BsiYI 286 CCACAGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
948 CCCAGACATGG
BslI 286 CCACAGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
948 CCCAGACATGG
BsmFI 528 GGGAC GGGAC(10/14)
537 GGGAC
Bsp1286I 761 GAGCTC G[AGT]GC[ACT]^C
963 GTGCCC
BstNI 27 CCAGG CC^[AT]GG
735 CCTGG
CfoI 63 GCGC GCG^C
958 GCGC
Cfr13I 591 GGTCC G^G[ACGT]CC
873 GGACC
DraII 590 AGGTCCT [GA]G^G[ACGT]CC[CT]
872 AGGACCC
EcoO109I 590 AGGTCCT [GA]G^G[ACGT]CC[CT]
872 AGGACCC
EcoRII 27 CCAGG ^CC[AT]GG
735 CCTGG
EcoRV 247 GATATC GAT^ATC
625 GATATC
EcoT14I 492 CCAAGG C^C[AT][AT]GG
878 CCATGG
HaeII 62 GGCGCT [GA]GCGC^[CT]
957 GGCGCT
HhaI 63 GCGC GCG^C
958 GCGC
HinP1I 63 GCGC G^CGC
958 GCGC
HphI 496 GGTGA GGTGA(8/7)
216 TCACC
MfeI 692 CAATTG C^AATTG
710 CAATTG
MunI 692 CAATTG C^AATTG
710 CAATTG
MvaI 27 CCAGG CC^[AT]GG
735 CCTGG
NlaIV 338 GGAACC GG[ACGT]^[ACGT]CC
873 GGACCC
PpuMI 590 AGGTCCT [GA]G^G[AT]CC[CT]
872 AGGACCC
PvuII 289 CAGCTG CAG^CTG
937 CAGCTG
Sau96I 591 GGTCC G^G[ACGT]CC
873 GGACC
SfcI 239 CTATAG C^T[GA][CT]AG
940 CTGCAG
StyI 492 CCAAGG C^C[AT][AT]GG
878 CCATGG
XcmI 440 CCAATACTCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
878 CCATGGACATAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BsaJI BsrI BssKI
BstF5I DdeI DpnII EarI Fnu4HI
FokI HinfI ItaI Ksp632I MboI
MboII MnlI MseI MspA1I MwoI
NdeII NlaIII RsaI Sau3AI ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I