Restriction map for
pFN21AE3537
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BsaBI BsaI BseAI
BsiEI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DraII EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoRI
EcoT22I FbaI FseI HgaI HindIII
HpaI KpnI KspI MamI MluI
MroI MvnI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacII SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 933 GTCGAC GT^[AC][GT]AC
AcyI 1430 GGCGCC G[GA]^CG[CT]C
Alw44I 10 GTGCAC G^TGCAC
AlwNI 652 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 10 GTGCAC G^TGCAC
AseI 868 ATTAAT AT^TAAT
AsnI 868 ATTAAT AT^TAAT
AspI 1245 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 450 CCTAGG C^CTAGG
BamHI 1210 GGATCC G^GATCC
BbeI 1430 GGCGCC GGCGC^C
BbvCI 1474 CCTCAGC CCTCAGC(-5/-2)
BbvI 1099 GCTGC GCAGC(8/12)
BcnI 379 CCCGG CC^[GC]GG
BfaI 451 CTAG C^TAG
BlnI 450 CCTAGG C^CTAGG
BlpI 1062 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1062 GCTGAGC GC^T[ACGT]AGC
BsaAI 628 TACGTG [CT]AC^GT[GA]
BsaHI 1430 GGCGCC G[GA]^CG[CT]C
BsaWI 335 ACCGGA [AT]^CCGG[AT]
BseRI 1399 GAGGAG GAGGAG(10/8)
BsgI 601 CTGCAC GTGCAG(16/14)
BsmI 1216 GCATTC GAATGC(1/-1)
BspHI 44 TCATGA T^CATGA
BssSI 30 CACGAG CACGAG(-5/-1)
BstXI 275 CCATGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1210 GGATCC [GA]^GATC[CT]
CelII 1062 GCTGAGC GC^T[ACGT]AGC
DpnII 1211 GATC ^GATC
DraIII 721 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1503 GACAAATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 1482 CCCGC CCCGC(4/6)
HaeII 1430 GGCGCC [GA]GCGC^[CT]
Hin1I 1430 GGCGCC G[GA]^CG[CT]C
KasI 1430 GGCGCC G^GCGCC
MaeI 451 CTAG C^TAG
MboI 1211 GATC ^GATC
MfeI 774 CAATTG C^AATTG
MflI 1210 GGATCC [GA]^GATC[CT]
MslI 276 CATGAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 411 CAGCGG C[AC]G^C[GT]G
MunI 774 CAATTG C^AATTG
NarI 1430 GGCGCC GG^CGCC
NciI 379 CCCGG CC^[GC]GG
NdeI 89 CATATG CA^TATG
NdeII 1211 GATC ^GATC
NspI 539 GCATGT [GA]CATG^[CT]
PaeR7I 1382 CTCGAG C^TCGAG
PflFI 1245 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 868 ATTAAT AT^TAAT
RcaI 44 TCATGA T^CATGA
SalI 933 GTCGAC G^TCGAC
Sau3AI 1211 GATC ^GATC
ScaI 1086 AGTACT AGT^ACT
SfcI 743 CTACAG C^T[GA][CT]AG
SfoI 1430 GGCGCC GGC^GCC
TfiI 765 GATTC G^A[AT]TC
TseI 1099 GCTGC G^C[AT]GC
Tth111I 1245 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 148 CCACCCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1382 CTCGAG C^TCGAG
XhoII 1210 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 70 GAATTT [GA]^AATT[CT]
339 GAATTT
AlwI 1211 GATCC GGATC(4/5)
1210 GGATC
AocI 1110 CCTGAGG CC^T[ACGT]AGG
1160 CCTCAGG
ApoI 70 GAATTT [GA]^AATT[CT]
339 GAATTT
AviII 856 TGCGCA TGC^GCA
965 TGCGCA
BalI 668 TGGCCA TGG^CCA
1016 TGGCCA
BanI 633 GGTGCC G^G[CT][GA]CC
1430 GGCGCC
BbsI 190 GAAGAC GAAGAC(2/6)
546 GAAGAC
BpuAI 190 GAAGAC GAAGAC(2/6)
546 GAAGAC
BspMI 599 ACCTGC ACCTGC(4/8)
219 GCAGGT
BstEII 222 GGTGACC G^GT[ACGT]ACC
807 GGTGACC
BstF5I 1421 CATCC GGATG(2/0)
1265 GGATG
BstPI 222 GGTGACC G^GT[ACGT]ACC
807 GGTGACC
Bsu36I 1110 CCTGAGG CC^T[ACGT]AGG
1160 CCTCAGG
DsaI 62 CCATGG C^C[GA][CT]GG
950 CCATGG
EaeI 668 TGGCCA [CT]^GGCC[GA]
1016 TGGCCA
Eco81I 1110 CCTGAGG CC^T[ACGT]AGG
1160 CCTCAGG
EcoNI 132 CCTTGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
213 CCTCCAGCAGG
EcoO65I 222 GGTGACC G^GT[ACGT]ACC
807 GGTGACC
EcoRV 184 GATATC GAT^ATC
406 GATATC
FokI 1421 CATCC GGATG(9/13)
1265 GGATG
FspI 856 TGCGCA TGC^GCA
965 TGCGCA
HapII 336 CCGG C^CGG
380 CCGG
HincII 514 GTCAAC GT[CT]^[GA]AC
933 GTCGAC
HindII 514 GTCAAC GT[CT]^[GA]AC
933 GTCGAC
HpaII 336 CCGG C^CGG
380 CCGG
HphI 222 GGTGA GGTGA(8/7)
807 GGTGA
MluNI 668 TGGCCA TGG^CCA
1016 TGGCCA
MscI 668 TGGCCA TGG^CCA
1016 TGGCCA
MspI 336 CCGG C^CGG
380 CCGG
NcoI 62 CCATGG C^CATGG
950 CCATGG
StuI 707 AGGCCT AGG^CCT
1471 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BanII BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI EarI EcoRII
EcoT14I Fnu4HI HaeIII HhaI HinP1I
HinfI ItaI Ksp632I MaeII MaeIII
MboII MnlI MseI MvaI MwoI
NlaIII NlaIV PflMI PleI RsaI
SacI SapI Sau96I ScrFI SfaNI
SmlI StyI TaqI Tru9I Tsp45I
Tsp509I TspRI TthHB8I Van91I