Restriction map for
pFN21AE6517
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
KspI MaeI MaeIII MamI MfeI
MflI MluI MroI MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 174 GTAC GT^AC
AflII 69 CTTAAG C^TTAAG
AluI 597 AGCT AG^CT
AocI 30 CCTGAGG CC^T[ACGT]AGG
AseI 238 ATTAAT AT^TAAT
AsnI 238 ATTAAT AT^TAAT
AspHI 562 GAGCAC G[AT]GC[AT]^C
AvaII 414 GGACC G^G[AT]CC
BalI 623 TGGCCA TGG^CCA
BanII 199 GAGCCC G[GA]GC[CT]^C
BbvI 422 GCAGC GCAGC(8/12)
BfrI 69 CTTAAG C^TTAAG
BsiHKAI 562 GAGCAC G[AT]GC[AT]^C
BsiYI 154 CCCACCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 154 CCCACCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrDI 376 GCAATG GCAATG(2/0)
Bsu36I 30 CCTGAGG CC^T[ACGT]AGG
Cac8I 54 GCAAGC GC[ACGT]^[ACGT]GC
DpnII 474 GATC ^GATC
DraI 270 TTTAAA TTT^AAA
DsaI 159 CCACGG C^C[GA][CT]GG
EaeI 623 TGGCCA [CT]^GGCC[GA]
EarI 196 GAAGAG CTCTTC(1/4)
Eco81I 30 CCTGAGG CC^T[ACGT]AGG
EcoRI 116 GAATTC G^AATTC
Fnu4HI 422 GCAGC GC^[ACGT]GC
ItaI 422 GCAGC GC^[ACGT]GC
Ksp632I 196 GAAGAG CTCTTC(1/4)
MaeII 493 ACGT A^CGT
MboI 474 GATC ^GATC
MluNI 623 TGGCCA TGG^CCA
MscI 623 TGGCCA TGG^CCA
MslI 393 CATAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 477 CAGCGG C[AC]G^C[GT]G
MwoI 490 GCTACGTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 290 CATATG CA^TATG
NdeII 474 GATC ^GATC
NlaIII 457 CATG CATG^
NlaIV 7 GGAACC GG[ACGT]^[ACGT]CC
PacI 184 TTAATTAA TTAAT^TAA
PshBI 238 ATTAAT AT^TAAT
RsaI 174 GTAC GT^AC
SapI 196 GAAGAGC GCTCTTC(1/4)
Sau3AI 474 GATC ^GATC
SfcI 107 CTGTAG C^T[GA][CT]AG
SmlI 69 CTTAAG C^T[CT][GA]AG
SspI 274 AATATT AAT^ATT
TfiI 590 GATTC G^A[AT]TC
TseI 422 GCAGC G^C[AT]GC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 5 GCGG CCGC(-3/-1)
479 GCGG
AcsI 116 GAATTC [GA]^AATT[CT]
503 AAATTT
ApoI 116 GAATTC [GA]^AATT[CT]
503 AAATTT
BbsI 574 GAAGAC GAAGAC(2/6)
530 GTCTTC
BmyI 199 GAGCCC G[AGT]GC[ACT]^C
562 GAGCAC
BpuAI 574 GAAGAC GAAGAC(2/6)
530 GTCTTC
BsmFI 247 GGGAC GGGAC(10/14)
585 GGGAC
Bsp1286I 199 GAGCCC G[AGT]GC[ACT]^C
562 GAGCAC
BsrI 609 ACTGG ACTGG(1/-1)
321 CCAGT
BssKI 303 CCTGG ^CC[ACGT]GG
435 CCTGG
BstNI 303 CCTGG CC^[AT]GG
435 CCTGG
BstXI 159 CCACGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
236 CCATTAATTTGG
Cfr13I 137 GGCCC G^G[ACGT]CC
414 GGACC
EcoRII 303 CCTGG ^CC[AT]GG
435 CCTGG
EcoT14I 11 CCTTGG C^C[AT][AT]GG
417 CCAAGG
HaeIII 137 GGCC GG^CC
624 GGCC
MvaI 303 CCTGG CC^[AT]GG
435 CCTGG
PleI 439 GACTC GAGTC(4/5)
542 GAGTC
Sau96I 137 GGCCC G^G[ACGT]CC
414 GGACC
ScrFI 303 CCTGG CC^[ACGT]GG
435 CCTGG
StyI 11 CCTTGG C^C[AT][AT]GG
417 CCAAGG
TaqI 362 TCGA T^CGA
600 TCGA
TthHB8I 362 TCGA T^CGA
600 TCGA
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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BsaJI DdeI HinfI MboII MnlI
MseI Tru9I Tsp509I TspRI