Restriction map for
pFN21AE6485
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 1033 GTATAC GT^[AC][GT]AC
Alw44I 83 GTGCAC G^TGCAC
AocI 1710 CCTCAGG CC^T[ACGT]AGG
ApaLI 83 GTGCAC G^TGCAC
Asp700I 118 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 919 GGATAC GTATCC(6/5)
BclI 1356 TGATCA T^GATCA
BcnI 10 CCGGG CC^[GC]GG
BseRI 353 CTCCTC GAGGAG(10/8)
Bsp1407I 1282 TGTACA T^GTACA
BsrGI 1282 TGTACA T^GTACA
Bst1107I 1033 GTATAC GTA^TAC
BstXI 658 CCACTTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1033 GTATAC GTA^TAC
Bsu36I 1710 CCTCAGG CC^T[ACGT]AGG
Eco81I 1710 CCTCAGG CC^T[ACGT]AGG
EcoRV 730 GATATC GAT^ATC
FbaI 1356 TGATCA T^GATCA
HapII 10 CCGG C^CGG
HincII 1599 GTCAAC GT[CT]^[GA]AC
HindII 1599 GTCAAC GT[CT]^[GA]AC
HindIII 703 AAGCTT A^AGCTT
HpaII 10 CCGG C^CGG
MaeII 1702 ACGT A^CGT
MspI 10 CCGG C^CGG
NciI 10 CCGGG CC^[GC]GG
NheI 1575 GCTAGC G^CTAGC
NspI 482 ACATGC [GA]CATG^[CT]
PflMI 58 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 139 GACTC GAGTC(4/5)
SacI 871 GAGCTC GAGCT^C
SspBI 1282 TGTACA T^GTACA
SwaI 341 ATTTAAAT ATTT^AAAT
Van91I 58 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 118 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 179 GAATTT [GA]^AATT[CT]
345 AAATTC
AfaI 1283 GTAC GT^AC
1818 GTAC
AlwNI 1250 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
1744 CAGAACCTG
ApoI 179 GAATTT [GA]^AATT[CT]
345 AAATTC
AspHI 83 GTGCAC G[AT]GC[AT]^C
871 GAGCTC
AspI 154 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1701 GACGTTGTC
BbsI 1716 GAAGAC GAAGAC(2/6)
965 GTCTTC
BpmI 56 CTCCAG CTGGAG(16/14)
406 CTCCAG
BpuAI 1716 GAAGAC GAAGAC(2/6)
965 GTCTTC
BsgI 27 GTGCAG GTGCAG(16/14)
416 GTGCAG
BsiHKAI 83 GTGCAC G[AT]GC[AT]^C
871 GAGCTC
BsmFI 513 GGGAC GGGAC(10/14)
1707 GTCCC
BstYI 285 GGATCT [GA]^GATC[CT]
1462 GGATCT
MflI 285 GGATCT [GA]^GATC[CT]
1462 GGATCT
MslI 37 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1321 CACTGCCATG
NdeI 799 CATATG CA^TATG
1690 CATATG
PflFI 154 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1701 GACGTTGTC
PstI 859 CTGCAG CTGCA^G
1672 CTGCAG
RsaI 1283 GTAC GT^AC
1818 GTAC
SapI 121 GCTCTTC GCTCTTC(1/4)
873 GCTCTTC
SfcI 859 CTGCAG C^T[GA][CT]AG
1672 CTGCAG
TaqI 69 TCGA T^CGA
1350 TCGA
Tsp45I 152 GTGAC ^GT[GC]AC
1314 GTCAC
Tth111I 154 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1701 GACGTTGTC
TthHB8I 69 TCGA T^CGA
1350 TCGA
XhoII 285 GGATCT [GA]^GATC[CT]
1462 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AluI AlwI BanII BbvI BfaI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NcoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp509I TspRI