Restriction map for
pFN21AE5477
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KspI MaeII MamI
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 735 GGTACC G^GTACC
AcsI 818 GAATTT [GA]^AATT[CT]
AflII 757 CTTAAG C^TTAAG
AflIII 772 ACATGT A^C[GA][CT]GT
ApoI 818 GAATTT [GA]^AATT[CT]
Asp718I 735 GGTACC G^GTACC
AvaII 207 GGACC G^G[AT]CC
BanI 735 GGTACC G^G[CT][GA]CC
BbvI 253 GCTGC GCAGC(8/12)
BfaI 250 CTAG C^TAG
BfrI 757 CTTAAG C^TTAAG
BsgI 284 CTGCAC GTGCAG(16/14)
BsmFI 811 GTCCC GGGAC(10/14)
BsmI 370 GCATTC GAATGC(1/-1)
BspLU11I 772 ACATGT A^CATGT
BspMI 906 ACCTGC ACCTGC(4/8)
BsrDI 176 GCAATG GCAATG(2/0)
BsrI 128 ACTGG ACTGG(1/-1)
BssSI 322 CACGAG CACGAG(-5/-1)
BstYI 947 AGATCC [GA]^GATC[CT]
EcoT22I 368 ATGCAT ATGCA^T
Fnu4HI 253 GCTGC GC^[ACGT]GC
ItaI 253 GCTGC GC^[ACGT]GC
KpnI 735 GGTACC GGTAC^C
MaeI 250 CTAG C^TAG
MflI 947 AGATCC [GA]^GATC[CT]
MseI 758 TTAA T^TAA
NdeI 595 CATATG CA^TATG
NheI 249 GCTAGC G^CTAGC
NsiI 368 ATGCAT ATGCA^T
NspI 772 ACATGT [GA]CATG^[CT]
SexAI 263 ACCTGGT A^CC[AT]GGT
SmlI 757 CTTAAG C^T[CT][GA]AG
TaqI 639 TCGA T^CGA
TfiI 102 GATTC G^A[AT]TC
Tru9I 758 TTAA T^TAA
TseI 253 GCTGC G^C[AT]GC
Tsp45I 434 GTCAC ^GT[GC]AC
TthHB8I 639 TCGA T^CGA
XhoII 947 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 840 CCGC CCGC(-3/-1)
951 CCGC
AspHI 304 GTGCTC G[AT]GC[AT]^C
961 GTGCTC
BbsI 936 GAAGAC GAAGAC(2/6)
802 GTCTTC
BmyI 304 GTGCTC G[AGT]GC[ACT]^C
961 GTGCTC
BpmI 718 CTCCAG CTGGAG(16/14)
66 CTGGAG
BpuAI 936 GAAGAC GAAGAC(2/6)
802 GTCTTC
BsiHKAI 304 GTGCTC G[AT]GC[AT]^C
961 GTGCTC
BsiYI 264 CCTGGTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
753 CCGACTTAAGG
BslI 264 CCTGGTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
753 CCGACTTAAGG
Bsp1286I 304 GTGCTC G[AGT]GC[ACT]^C
961 GTGCTC
CfoI 480 GCGC GCG^C
910 GCGC
Cfr13I 207 GGACC G^G[ACGT]CC
750 GGCCC
DsaI 380 CCATGG C^C[GA][CT]GG
404 CCATGG
EarI 190 CTCTTC CTCTTC(1/4)
235 CTCTTC
EcoT14I 380 CCATGG C^C[AT][AT]GG
404 CCATGG
HhaI 480 GCGC GCG^C
910 GCGC
HinP1I 480 GCGC G^CGC
910 GCGC
Ksp632I 190 CTCTTC CTCTTC(1/4)
235 CTCTTC
MaeIII 181 GTTAC ^GT[ACGT]AC
434 GTCAC
NcoI 380 CCATGG C^CATGG
404 CCATGG
NlaIV 207 GGACCC GG[ACGT]^[ACGT]CC
735 GGTACC
PleI 939 GACTC GAGTC(4/5)
385 GAGTC
Sau96I 207 GGACC G^G[ACGT]CC
750 GGCCC
StyI 380 CCATGG C^C[AT][AT]GG
404 CCATGG
Tsp509I 58 AATT ^AATT
819 AATT
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI AlwI BalI BsaJI
BseRI BssKI BstF5I BstNI BstXI
Cac8I DdeI DpnII EaeI EcoRII
FokI HaeIII HinfI HphI MboI
MboII MluNI MnlI MscI MslI
MvaI MwoI NdeII NlaIII RsaI
Sau3AI ScrFI SfaNI TspRI