Restriction map for
pFN21AE6069
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AvaI AvaII AviII
AvrII BanI BanII BbeI BbrPI
BbvI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI Fnu4HI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
ItaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MroI
MspA1I MunI MvnI MwoI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StyI SwaI TaqI TseI
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 792 CCGC CCGC(-3/-1)
AflIII 548 ACATGT A^C[GA][CT]GT
AhdI 544 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 544 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 225 GTGCTC G[AT]GC[AT]^C
AspI 910 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 923 GGATCC G^GATCC
BbsI 67 GAAGAC GAAGAC(2/6)
BbvCI 692 CCTCAGC CCTCAGC(-5/-2)
BciVI 942 GGATAC GTATCC(6/5)
BcnI 672 CCGGG CC^[GC]GG
BfaI 230 CTAG C^TAG
BmyI 225 GTGCTC G[AGT]GC[ACT]^C
BpmI 764 CTCCAG CTGGAG(16/14)
BpuAI 67 GAAGAC GAAGAC(2/6)
BsiHKAI 225 GTGCTC G[AT]GC[AT]^C
BsmAI 908 GAGAC GTCTC(1/5)
BsmI 292 GAATGC GAATGC(1/-1)
Bsp1286I 225 GTGCTC G[AGT]GC[ACT]^C
BspLU11I 548 ACATGT A^CATGT
BspMI 724 ACCTGC ACCTGC(4/8)
BsrI 63 ACTGG ACTGG(1/-1)
BstXI 789 CCACCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 923 GGATCC [GA]^GATC[CT]
Cac8I 500 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 498 GCGC GCG^C
Cfr13I 360 GGCCC G^G[ACGT]CC
Eam1105I 544 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 497 AGCGCC [GA]GCGC^[CT]
HapII 672 CCGG C^CGG
HhaI 498 GCGC GCG^C
HinP1I 498 GCGC G^CGC
HpaII 672 CCGG C^CGG
MaeI 230 CTAG C^TAG
MaeIII 542 GTGAC ^GT[ACGT]AC
MflI 923 GGATCC [GA]^GATC[CT]
MseI 719 TTAA T^TAA
MspI 672 CCGG C^CGG
NciI 672 CCGGG CC^[GC]GG
NlaIV 923 GGATCC GG[ACGT]^[ACGT]CC
NspI 548 ACATGT [GA]CATG^[CT]
PflFI 910 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 510 CTGCAG CTGCA^G
Sau96I 360 GGCCC G^G[ACGT]CC
SmlI 271 CTCAAG C^T[CT][GA]AG
SspI 841 AATATT AAT^ATT
StuI 713 AGGCCT AGG^CCT
TfiI 666 GATTC G^A[AT]TC
Tru9I 719 TTAA T^TAA
Tsp45I 542 GTGAC ^GT[GC]AC
Tth111I 910 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 923 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 183 GTAC GT^AC
233 GTAC
AluI 33 AGCT AG^CT
768 AGCT
BalI 194 TGGCCA TGG^CCA
377 TGGCCA
BsaJI 57 CCCAGG C^C[ACGT][ACGT]GG
362 CCCTGG
BseRI 139 CTCCTC GAGGAG(10/8)
690 CTCCTC
BsgI 169 CTGCAC GTGCAG(16/14)
726 CTGCAC
BsmFI 439 GGGAC GGGAC(10/14)
55 GTCCC
BstNI 58 CCAGG CC^[AT]GG
363 CCTGG
DpnII 367 GATC ^GATC
924 GATC
EaeI 194 TGGCCA [CT]^GGCC[GA]
377 TGGCCA
EcoRII 58 CCAGG ^CC[AT]GG
363 CCTGG
MboI 367 GATC ^GATC
924 GATC
MluNI 194 TGGCCA TGG^CCA
377 TGGCCA
MscI 194 TGGCCA TGG^CCA
377 TGGCCA
MslI 327 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
736 CACATTTGTG
MvaI 58 CCAGG CC^[AT]GG
363 CCTGG
NdeII 367 GATC ^GATC
924 GATC
RsaI 183 GTAC GT^AC
233 GTAC
Sau3AI 367 GATC ^GATC
924 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AlwI BsiYI BslI BssKI BstF5I
DdeI FokI HaeIII HinfI HphI
MboII MnlI NlaIII PleI ScrFI
SfaNI SfcI Tsp509I TspRI