Restriction map for
pFN21AE3950
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# NO CUT ENZYMES ( 91 enzymes) #
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AccI AccIII AclI AcsI AflII
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BamHI BcgI BciVI BfaI
BfrI BlnI BsaBI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HpaI
MaeI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tsp509I XbaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 563 GGTACC G^GTACC
AflIII 904 ACGTGT A^C[GA][CT]GT
AgeI 1973 ACCGGT A^CCGGT
Alw44I 1948 GTGCAC G^TGCAC
AlwNI 127 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1948 GTGCAC G^TGCAC
Asp718I 563 GGTACC G^GTACC
AspI 2242 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2262 CCTCAGC CCTCAGC(-5/-2)
BglII 1373 AGATCT A^GATCT
BlpI 303 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 303 GCTCAGC GC^T[ACGT]AGC
BsaI 287 GAGACC GGTCTC(1/5)
Bsp1407I 311 TGTACA T^GTACA
BsrBI 142 CCGCTC CCGCTC(-3/-3)
BsrGI 311 TGTACA T^GTACA
BssSI 1003 CACGAG CACGAG(-5/-1)
BstEII 2286 GGTCACC G^GT[ACGT]ACC
BstPI 2286 GGTCACC G^GT[ACGT]ACC
BstXI 2066 CCATGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 303 GCTCAGC GC^T[ACGT]AGC
DraIII 1003 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 978 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2286 GGTCACC G^GT[ACGT]ACC
HindIII 1757 AAGCTT A^AGCTT
HinfI 1751 GAGTC G^A[ACGT]TC
KpnI 563 GGTACC GGTAC^C
NaeI 989 GCCGGC GCC^GGC
NcoI 386 CCATGG C^CATGG
NgoMIV 989 GCCGGC G^CCGGC
NspI 935 GCATGT [GA]CATG^[CT]
PaeR7I 544 CTCGAG C^TCGAG
PflFI 2242 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1500 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1973 ACCGGT A^CCGGT
PleI 1751 GAGTC GAGTC(4/5)
SacI 462 GAGCTC GAGCT^C
SapI 7 GAAGAGC GCTCTTC(1/4)
ScaI 1325 AGTACT AGT^ACT
SspBI 311 TGTACA T^GTACA
Tth111I 2242 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1500 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 544 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AatII 594 GACGTC GACGT^C
679 GACGTC
AlwI 1134 GATCC GGATC(4/5)
160 GGATC
ApaI 796 GGGCCC GGGCC^C
2217 GGGCCC
BalI 614 TGGCCA TGG^CCA
2009 TGGCCA
BclI 1340 TGATCA T^GATCA
1388 TGATCA
BsaWI 1312 ACCGGA [AT]^CCGG[AT]
1973 ACCGGT
BsiEI 633 CGACCG CG[GA][CT]^CG
1908 CGACCG
BstAPI 126 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
935 GCATGTACTGC
BstYI 1133 AGATCC [GA]^GATC[CT]
1373 AGATCT
EarI 1767 CTCTTC CTCTTC(1/4)
7 GAAGAG
FbaI 1340 TGATCA T^GATCA
1388 TGATCA
Ksp632I 1767 CTCTTC CTCTTC(1/4)
7 GAAGAG
MflI 1133 AGATCC [GA]^GATC[CT]
1373 AGATCT
MluNI 614 TGGCCA TGG^CCA
2009 TGGCCA
MscI 614 TGGCCA TGG^CCA
2009 TGGCCA
MseI 1063 TTAA T^TAA
1201 TTAA
PpuMI 337 GGGTCCT [GA]G^G[AT]CC[CT]
726 GGGACCC
StuI 1058 AGGCCT AGG^CCT
2057 AGGCCT
Tru9I 1063 TTAA T^TAA
1201 TTAA
XhoII 1133 AGATCC [GA]^GATC[CT]
1373 AGATCT
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbeI BbrPI BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI KspI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PmaCI PmlI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XmaCI XmaI