Restriction map for
pFN21AE5129
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# NO CUT ENZYMES ( 188 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AluI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tru9I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 34 GTCGAC GT^[AC][GT]AC
AcsI 244 AAATTT [GA]^AATT[CT]
AfaI 438 GTAC GT^AC
AflIII 402 ACGTGT A^C[GA][CT]GT
AlwNI 278 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 244 AAATTT [GA]^AATT[CT]
Asp700I 100 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 310 GGACC G^G[AT]CC
BbvI 346 GCTGC GCAGC(8/12)
BglI 206 GCCATAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 122 CTGGAG CTGGAG(16/14)
BsiYI 230 CCATCTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 230 CCATCTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI 471 CATTGC GCAATG(2/0)
BssKI 172 CCAGG ^CC[ACGT]GG
BstNI 172 CCAGG CC^[AT]GG
DpnII 465 GATC ^GATC
DrdI 25 GACATCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 172 CCAGG ^CC[AT]GG
HinfI 84 GATTC G^A[ACGT]TC
MaeII 402 ACGT A^CGT
MboI 465 GATC ^GATC
MboII 462 GAAGA GAAGA(8/7)
MnlI 261 CCTC CCTC(7/6)
MspA1I 95 CAGCGG C[AC]G^C[GT]G
MvaI 172 CCAGG CC^[AT]GG
NdeII 465 GATC ^GATC
NspI 375 GCATGT [GA]CATG^[CT]
RsaI 438 GTAC GT^AC
SalI 34 GTCGAC G^TCGAC
Sau3AI 465 GATC ^GATC
ScrFI 172 CCAGG CC^[ACGT]GG
SspI 409 AATATT AAT^ATT
TaqI 35 TCGA T^CGA
TfiI 84 GATTC G^A[AT]TC
TseI 346 GCTGC G^C[AT]GC
TthHB8I 35 TCGA T^CGA
XcmI 207 CCATAATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 100 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 54 CCGC CCGC(-3/-1)
97 GCGG
BstF5I 264 CATCC GGATG(2/0)
366 GGATG
Cac8I 41 GCAAGC GC[ACGT]^[ACGT]GC
371 GCAAGC
Cfr13I 51 GGGCC G^G[ACGT]CC
310 GGACC
DdeI 115 CTGAG C^T[ACGT]AG
356 CTGAG
DsaI 296 CCATGG C^C[GA][CT]GG
422 CCATGG
Fnu4HI 53 GCCGC GC^[ACGT]GC
346 GCTGC
FokI 264 CATCC GGATG(9/13)
366 GGATG
HaeIII 52 GGCC GG^CC
381 GGCC
HincII 4 GTCAAC GT[CT]^[GA]AC
34 GTCGAC
HindII 4 GTCAAC GT[CT]^[GA]AC
34 GTCGAC
ItaI 53 GCCGC GC^[ACGT]GC
346 GCTGC
MaeIII 194 GTGAC ^GT[ACGT]AC
221 GTGAC
MwoI 206 GCCATAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
449 GCAAGACCAGC
NcoI 296 CCATGG C^CATGG
422 CCATGG
Sau96I 51 GGGCC G^G[ACGT]CC
310 GGACC
SfaNI 56 GCATC GCATC(5/9)
287 GCATC
Tsp45I 194 GTGAC ^GT[GC]AC
221 GTGAC
TspRI 120 CACTG CA[GC]TG[ACGT][ACGT]^
216 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AspHI BmyI BsaJI BsiHKAI Bsp1286I
BsrI EcoT14I HphI NlaIII NlaIV
StyI Tsp509I