##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaJI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr13I      
ClaI        CpoI        DpnII       DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoRV       EcoT14I     
EcoT22I     FbaI        Fnu4HI      FokI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        ItaI        
KasI        KpnI        KspI        MaeII       MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvaI        MvnI        MwoI        NarI        NciI        
NcoI        NdeI        NdeII       NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TseI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AcsI          256  GAATTC               [GA]^AATT[CT]
ApoI          256  GAATTC               [GA]^AATT[CT]
AvaI          215  CCCGAG               C^[CT]CG[GA]G
BfaI          151  CTAG                 C^TAG
BpmI          188  CTCCAG               CTGGAG(16/14)
BsaI           79  GAGACC               GGTCTC(1/5)
BsmAI          79  GAGAC                GTCTC(1/5)
BsoBI         215  CCCGAG               C^[CT]CG[GA]G
BsrFI          19  GCCGGC               [GA]^CCGG[CT]
BstXI         146  CCATACTAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          19  GCCGGC               GC[ACGT]^[ACGT]GC
Cfr10I         19  GCCGGC               [GA]^CCGG[CT]
DraI          175  TTTAAA               TTT^AAA
EarI           30  GAAGAG               CTCTTC(1/4)
EcoRI         256  GAATTC               G^AATTC
HaeIII         60  GGCC                 GG^CC
HgaI           38  GCGTC                GACGC(5/10)
HphI           27  GGTGA                GGTGA(8/7)
Ksp632I        30  GAAGAG               CTCTTC(1/4)
MaeI          151  CTAG                 C^TAG
MboII          30  GAAGA                GAAGA(8/7)
MseI          176  TTAA                 T^TAA
MslI          147  CATACTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI           19  GCCGGC               GCC^GGC
NgoMIV         19  GCCGGC               G^CCGGC
NlaIII        261  CATG                 CATG^
SfaNI          72  GATGC                GCATC(5/9)
SfcI          162  CTACAG               C^T[GA][CT]AG
SpeI          150  ACTAGT               A^CTAGT
TaqI          142  TCGA                 T^CGA
Tru9I         176  TTAA                 T^TAA
Tsp509I       257  AATT                 ^AATT
TspRI          15  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       142  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AciI          103  CCGC                 CCGC(-3/-1)
               23  GCGG                
BsmFI          92  GGGAC                GGGAC(10/14)
               40  GTCCC               
BspMI         108  ACCTGC               ACCTGC(4/8)
              282  ACCTGC              
BsrI           88  ACTGG                ACTGG(1/-1)
               14  CCAGT               
DdeI           96  CTCAG                C^T[ACGT]AG
              134  CTGAG               
FauI          102  CCCGC                CCCGC(4/6)
               23  GCGGG               
HapII          20  CCGG                 C^CGG
               76  CCGG                
HpaII          20  CCGG                 C^CGG
               76  CCGG                
MspI           20  CCGG                 C^CGG
               76  CCGG                
PleI           94  GACTC                GAGTC(4/5)
              136  GAGTC               
TfiI           49  GATTC                G^A[AT]TC
              227  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

BseRI       BsiYI       BslI        HinfI       MnlI