Restriction map for
pFN21AE1110
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SmlI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AvaI 82 CCCGAG C^[CT]CG[GA]G
BamHI 150 GGATCC G^GATCC
BfaI 32 CTAG C^TAG
BglI 72 GCCTATGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 46 TACGTA [CT]AC^GT[GA]
BseRI 304 GAGGAG GAGGAG(10/8)
BsoBI 82 CCCGAG C^[CT]CG[GA]G
BspMI 193 GCAGGT ACCTGC(4/8)
BsrI 247 ACTGG ACTGG(1/-1)
BssKI 225 CCAGG ^CC[ACGT]GG
BstEII 235 GGTCACC G^GT[ACGT]ACC
BstF5I 177 GGATG GGATG(2/0)
BstNI 225 CCAGG CC^[AT]GG
BstPI 235 GGTCACC G^GT[ACGT]ACC
BstYI 150 GGATCC [GA]^GATC[CT]
DdeI 164 CTCAG C^T[ACGT]AG
EcoO65I 235 GGTCACC G^GT[ACGT]ACC
EcoRII 225 CCAGG ^CC[AT]GG
FauI 291 CCCGC CCCGC(4/6)
FokI 177 GGATG GGATG(9/13)
HgaI 41 GACGC GACGC(5/10)
MaeI 32 CTAG C^TAG
MaeIII 236 GTCAC ^GT[ACGT]AC
MflI 150 GGATCC [GA]^GATC[CT]
MspA1I 158 CAGCTG C[AC]G^C[GT]G
MvaI 225 CCAGG CC^[AT]GG
PflMI 241 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 158 CAGCTG CAG^CTG
ScrFI 225 CCAGG CC^[ACGT]GG
SfaNI 187 GCATC GCATC(5/9)
SfiI 71 GGCCTATGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 46 TACGTA TAC^GTA
TaqI 19 TCGA T^CGA
Tsp45I 236 GTCAC ^GT[GC]AC
TthHB8I 19 TCGA T^CGA
Van91I 241 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 150 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 292 CCGC CCGC(-3/-1)
78 GCGG
AluI 159 AGCT AG^CT
216 AGCT
AlwI 151 GATCC GGATC(4/5)
150 GGATC
AvaII 268 GGTCC G^G[AT]CC
288 GGACC
BbvI 157 GCAGC GCAGC(8/12)
160 GCTGC
DpnII 17 GATC ^GATC
151 GATC
EcoNI 118 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
319 CCTGAAGAAGG
MaeII 47 ACGT A^CGT
144 ACGT
MboI 17 GATC ^GATC
151 GATC
MwoI 72 GCCTATGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
78 GCGGCCCGAGC
NdeII 17 GATC ^GATC
151 GATC
PstI 155 CTGCAG CTGCA^G
191 CTGCAG
Sau3AI 17 GATC ^GATC
151 GATC
SfcI 155 CTGCAG C^T[GA][CT]AG
191 CTGCAG
TseI 157 GCAGC G^C[AT]GC
160 GCTGC
TspRI 93 CAGTG CA[GC]TG[ACGT][ACGT]^
343 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BsiYI BslI Cfr13I Fnu4HI HaeIII
HphI ItaI MboII MnlI NlaIV
Sau96I