Restriction map for
pFN21AE2031
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AocI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BanI BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
Eam1105I Eco81I EcoO65I EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KasI KpnI MaeI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI RcaI
SacI SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 1087 GTCTAC GT^[AC][GT]AC
AcsI 427 GAATTC [GA]^AATT[CT]
Aor51HI 1549 AGCGCT AGC^GCT
ApaI 236 GGGCCC GGGCC^C
ApoI 427 GAATTC [GA]^AATT[CT]
BglI 1837 GCCATCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1233 AGATCT A^GATCT
BlpI 1552 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1552 GCTGAGC GC^T[ACGT]AGC
BssHII 1701 GCGCGC G^CGCGC
BstBI 703 TTCGAA TT^CGAA
CelII 1552 GCTGAGC GC^T[ACGT]AGC
CpoI 1488 CGGACCG CG^G[AT]CCG
DraI 124 TTTAAA TTT^AAA
EagI 689 CGGCCG C^GGCCG
EarI 1686 GAAGAG CTCTTC(1/4)
EclXI 689 CGGCCG C^GGCCG
Eco47III 1549 AGCGCT AGC^GCT
Eco52I 689 CGGCCG C^GGCCG
EcoNI 1674 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 427 GAATTC G^AATTC
FseI 1647 GGCCGGCC GGCCGG^CC
Ksp632I 1686 GAAGAG CTCTTC(1/4)
KspI 692 CCGCGG CCGC^GG
NaeI 1648 GCCGGC GCC^GGC
NgoMIV 1648 GCCGGC G^CCGGC
NruI 374 TCGCGA TCG^CGA
NspV 703 TTCGAA TT^CGAA
RsrII 1488 CGGACCG CG^G[AT]CCG
SacII 692 CCGCGG CCGC^GG
SexAI 1799 ACCAGGT A^CC[AT]GGT
SfcI 321 CTACAG C^T[GA][CT]AG
SfuI 703 TTCGAA TT^CGAA
SphI 548 GCATGC GCATG^C
Tsp509I 428 AATT ^AATT
XcmI 1031 CCATGGGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 316 GACGCC G[GA]^CG[CT]C
985 GACGCC
Alw44I 1388 GTGCAC G^TGCAC
1813 GTGCAC
ApaLI 1388 GTGCAC G^TGCAC
1813 GTGCAC
Asp700I 603 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
921 GAAGGTGTTC
AspHI 1388 GTGCAC G[AT]GC[AT]^C
1813 GTGCAC
AspI 244 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1597 GACAAAGTC
AvaI 830 CTCGGG C^[CT]CG[GA]G
1397 CCCGAG
BamHI 530 GGATCC G^GATCC
1574 GGATCC
BbsI 706 GAAGAC GAAGAC(2/6)
1047 GAAGAC
BbvCI 81 CCTCAGC CCTCAGC(-5/-2)
1275 GCTGAGG
BpuAI 706 GAAGAC GAAGAC(2/6)
1047 GAAGAC
BsaHI 316 GACGCC G[GA]^CG[CT]C
985 GACGCC
BsiHKAI 1388 GTGCAC G[AT]GC[AT]^C
1813 GTGCAC
BsmBI 1267 GAGACG CGTCTC(1/5)
1666 GAGACG
BsoBI 830 CTCGGG C^[CT]CG[GA]G
1397 CCCGAG
BsrBI 257 GAGCGG CCGCTC(-3/-3)
1750 GAGCGG
BsrI 1392 ACTGG ACTGG(1/-1)
1586 ACTGG
BstAPI 1494 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1806 GCAGGTGGTGC
BstXI 974 CCAGCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1847 CCACCAAAGTGG
DraIII 355 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1798 CACCAGGTG
DrdI 412 GACACCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1594 GACGACAAAGTC
HaeII 55 AGCGCC [GA]GCGC^[CT]
1549 AGCGCT
Hin1I 316 GACGCC G[GA]^CG[CT]C
985 GACGCC
MseI 125 TTAA T^TAA
937 TTAA
NspI 548 GCATGC [GA]CATG^[CT]
1724 ACATGC
PflFI 244 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1597 GACAAAGTC
PflMI 402 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
534 CCAGGCCCTGG
PpuMI 653 AGGACCT [GA]G^G[AT]CC[CT]
1535 GGGACCC
PvuI 266 CGATCG CGAT^CG
377 CGATCG
PvuII 406 CAGCTG CAG^CTG
619 CAGCTG
TfiI 288 GATTC G^A[AT]TC
593 GATTC
Tru9I 125 TTAA T^TAA
937 TTAA
Tth111I 244 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1597 GACAAAGTC
Van91I 402 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
534 CCAGGCCCTGG
XmnI 603 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
921 GAAGGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BalI BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiEI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII