Restriction map for
pFN21AE3682
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbeI BbrPI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspEI BspHI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I TspRI
Tth111I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AflIII 569 ACATGT A^C[GA][CT]GT
AlwI 504 GATCC GGATC(4/5)
AlwNI 1015 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 713 GGGCCC GGGCC^C
Asp700I 603 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 214 GAGCAC G[AT]GC[AT]^C
AviII 1022 TGCGCA TGC^GCA
BalI 356 TGGCCA TGG^CCA
BbvCI 912 CCTCAGC CCTCAGC(-5/-2)
BcgI 795 GCATCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 151 CTAG C^TAG
BsiEI 1039 CGACCG CG[GA][CT]^CG
BsiHKAI 214 GAGCAC G[AT]GC[AT]^C
BsmAI 768 GAGAC GTCTC(1/5)
BsmBI 768 GAGACG CGTCTC(1/5)
BspDI 79 ATCGAT AT^CGAT
BspLU11I 569 ACATGT A^CATGT
BsrBI 1006 GAGCGG CCGCTC(-3/-3)
BsrFI 315 GCCGGC [GA]^CCGG[CT]
BstYI 503 AGATCC [GA]^GATC[CT]
Cfr10I 315 GCCGGC [GA]^CCGG[CT]
ClaI 79 ATCGAT AT^CGAT
DpnII 504 GATC ^GATC
DsaI 353 CCATGG C^C[GA][CT]GG
EarI 800 CTCTTC CTCTTC(1/4)
EcoNI 87 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 962 ATGCAT ATGCA^T
FspI 1022 TGCGCA TGC^GCA
HinfI 55 GACTC G^A[ACGT]TC
HphI 350 TCACC GGTGA(8/7)
Ksp632I 800 CTCTTC CTCTTC(1/4)
MaeI 151 CTAG C^TAG
MboI 504 GATC ^GATC
MflI 503 AGATCC [GA]^GATC[CT]
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MseI 500 TTAA T^TAA
MslI 348 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 315 GCCGGC GCC^GGC
NcoI 353 CCATGG C^CATGG
NdeII 504 GATC ^GATC
NgoMIV 315 GCCGGC G^CCGGC
NsiI 962 ATGCAT ATGCA^T
NspI 569 ACATGT [GA]CATG^[CT]
PleI 55 GACTC GAGTC(4/5)
Sau3AI 504 GATC ^GATC
Tru9I 500 TTAA T^TAA
Tsp509I 1145 AATT ^AATT
XhoII 503 AGATCC [GA]^GATC[CT]
XmnI 603 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 770 GACGCC G[GA]^CG[CT]C
941 GGCGTC
AfaI 336 GTAC GT^AC
1012 GTAC
AvaII 552 GGACC G^G[AT]CC
909 GGTCC
BanII 635 GAGCCC G[GA]GC[CT]^C
713 GGGCCC
BglI 521 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1236 GCCTCGTCGGC
BlpI 16 GCTGAGC GC^T[ACGT]AGC
390 GCTCAGC
Bpu1102I 16 GCTGAGC GC^T[ACGT]AGC
390 GCTCAGC
BsaHI 770 GACGCC G[GA]^CG[CT]C
941 GGCGTC
BsrI 542 ACTGG ACTGG(1/-1)
733 CCAGT
CelII 16 GCTGAGC GC^T[ACGT]AGC
390 GCTCAGC
EaeI 356 TGGCCA [CT]^GGCC[GA]
417 TGGCCG
HgaI 770 GACGC GACGC(5/10)
942 GCGTC
Hin1I 770 GACGCC G[GA]^CG[CT]C
941 GGCGTC
NlaIII 354 CATG CATG^
570 CATG
PstI 175 CTGCAG CTGCA^G
894 CTGCAG
RsaI 336 GTAC GT^AC
1012 GTAC
SfcI 175 CTGCAG C^T[GA][CT]AG
894 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AvaI BanI
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI SfaNI SmaI
StyI TaqI TseI TthHB8I XmaCI
XmaI