Restriction map for
pFN21AE5807
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AccIII AgeI AhdI
Aor51HI AscI AspEI AspI AvrII
BamHI BbeI BcgI BglI BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BsrBI BssSI
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I FseI HaeII
HpaI KasI KspI MamI MluI
MroI NarI NdeI NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1341 GGTACC G^GTACC
AcyI 3937 GACGCC G[GA]^CG[CT]C
AflII 609 CTTAAG C^TTAAG
AflIII 69 ACGTGT A^C[GA][CT]GT
Alw44I 3794 GTGCAC G^TGCAC
AocI 2319 CCTGAGG CC^T[ACGT]AGG
ApaI 54 GGGCCC GGGCC^C
ApaLI 3794 GTGCAC G^TGCAC
AseI 2151 ATTAAT AT^TAAT
AsnI 2151 ATTAAT AT^TAAT
Asp718I 1341 GGTACC G^GTACC
AvaI 114 CTCGGG C^[CT]CG[GA]G
AviII 101 TGCGCA TGC^GCA
BanI 1341 GGTACC G^G[CT][GA]CC
BbrPI 68 CACGTG CAC^GTG
BclI 2372 TGATCA T^GATCA
BcnI 299 CCCGG CC^[GC]GG
BfrI 609 CTTAAG C^TTAAG
BlpI 324 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 324 GCTGAGC GC^T[ACGT]AGC
BsaAI 68 CACGTG [CT]AC^GT[GA]
BsaHI 3937 GACGCC G[GA]^CG[CT]C
BsaI 2209 GAGACC GGTCTC(1/5)
BsgI 759 CTGCAC GTGCAG(16/14)
BsmBI 394 CGTCTC CGTCTC(1/5)
BsoBI 114 CTCGGG C^[CT]CG[GA]G
BspMI 767 ACCTGC ACCTGC(4/8)
BssHII 242 GCGCGC G^CGCGC
BstAPI 993 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 294 GGTGACC G^GT[ACGT]ACC
BstPI 294 GGTGACC G^GT[ACGT]ACC
Bsu36I 2319 CCTGAGG CC^T[ACGT]AGG
CelII 324 GCTGAGC GC^T[ACGT]AGC
Eco81I 2319 CCTGAGG CC^T[ACGT]AGG
EcoO65I 294 GGTGACC G^GT[ACGT]ACC
EcoRI 619 GAATTC G^AATTC
EcoRV 3405 GATATC GAT^ATC
FbaI 2372 TGATCA T^GATCA
FspI 101 TGCGCA TGC^GCA
Hin1I 3937 GACGCC G[GA]^CG[CT]C
HincII 2890 GTCAAC GT[CT]^[GA]AC
HindII 2890 GTCAAC GT[CT]^[GA]AC
KpnI 1341 GGTACC GGTAC^C
NciI 299 CCCGG CC^[GC]GG
NcoI 248 CCATGG C^CATGG
PmaCI 68 CACGTG CAC^GTG
PmlI 68 CACGTG CAC^GTG
PshAI 2304 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2151 ATTAAT AT^TAAT
SacI 1557 GAGCTC GAGCT^C
StuI 110 AGGCCT AGG^CCT
SwaI 2768 ATTTAAAT ATTT^AAAT
XbaI 486 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
BalI 2426 TGGCCA TGG^CCA
3860 TGGCCA
BanII 54 GGGCCC G[GA]GC[CT]^C
1557 GAGCTC
BbvCI 237 CCTCAGC CCTCAGC(-5/-2)
2577 CCTCAGC
BciVI 3233 GGATAC GTATCC(6/5)
3073 GTATCC
Bsp1407I 684 TGTACA T^GTACA
4091 TGTACA
BspHI 1490 TCATGA T^CATGA
2805 TCATGA
BsrFI 124 GCCGGC [GA]^CCGG[CT]
194 GCCGGC
BsrGI 684 TGTACA T^GTACA
4091 TGTACA
Cfr10I 124 GCCGGC [GA]^CCGG[CT]
194 GCCGGC
DsaI 248 CCATGG C^C[GA][CT]GG
3229 CCACGG
EaeI 2426 TGGCCA [CT]^GGCC[GA]
3860 TGGCCA
EcoT22I 2345 ATGCAT ATGCA^T
3394 ATGCAT
HindIII 1365 AAGCTT A^AGCTT
3676 AAGCTT
MfeI 3286 CAATTG C^AATTG
3572 CAATTG
MluNI 2426 TGGCCA TGG^CCA
3860 TGGCCA
MscI 2426 TGGCCA TGG^CCA
3860 TGGCCA
MunI 3286 CAATTG C^AATTG
3572 CAATTG
NaeI 124 GCCGGC GCC^GGC
194 GCCGGC
NgoMIV 124 GCCGGC G^CCGGC
194 GCCGGC
NsiI 2345 ATGCAT ATGCA^T
3394 ATGCAT
PflMI 67 CCACGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3966 CCAGACCTTGG
PpuMI 61 AGGTCCC [GA]G^G[AT]CC[CT]
3782 AGGTCCT
PstI 991 CTGCAG CTGCA^G
1000 CTGCAG
RcaI 1490 TCATGA T^CATGA
2805 TCATGA
SpeI 711 ACTAGT A^CTAGT
4179 ACTAGT
SspBI 684 TGTACA T^GTACA
4091 TGTACA
SspI 2938 AATATT AAT^ATT
4017 AATATT
Van91I 67 CCACGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3966 CCAGACCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AclI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BbsI BbvI BfaI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI Psp1406I PvuII
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI