##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        Asp700I     
AspEI       AspI        AvaI        AvaII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        HaeII       HaeIII      
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HinfI       HpaI        HpaII       HphI        
KasI        Ksp632I     KspI        MamI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        234  GGTACC               G^GTACC
AfaI          235  GTAC                 GT^AC
AflIII        180  ACACGT               A^C[GA][CT]GT
AlwI          129  GGATC                GGATC(4/5)
AseI          108  ATTAAT               AT^TAAT
AsnI          108  ATTAAT               AT^TAAT
Asp718I       234  GGTACC               G^GTACC
AspHI         256  GAGCTC               G[AT]GC[AT]^C
AviII          27  TGCGCA               TGC^GCA
BanI          234  GGTACC               G^G[CT][GA]CC
BbvI           40  GCAGC                GCAGC(8/12)
BfaI          175  CTAG                 C^TAG
BlpI          286  GCTCAGC              GC^T[ACGT]AGC
BpmI           68  CTGGAG               CTGGAG(16/14)
Bpu1102I      286  GCTCAGC              GC^T[ACGT]AGC
BsaJI         230  CCCTGG               C^C[ACGT][ACGT]GG
BsiHKAI       256  GAGCTC               G[AT]GC[AT]^C
BsrI           67  ACTGG                ACTGG(1/-1)
BssKI         231  CCTGG                ^CC[ACGT]GG
BstNI         231  CCTGG                CC^[AT]GG
CelII         286  GCTCAGC              GC^T[ACGT]AGC
CfoI           28  GCGC                 GCG^C
DpnII         130  GATC                 ^GATC
EcoRII        231  CCTGG                ^CC[AT]GG
Fnu4HI         40  GCAGC                GC^[ACGT]GC
FspI           27  TGCGCA               TGC^GCA
HhaI           28  GCGC                 GCG^C
HinP1I         28  GCGC                 G^CGC
ItaI           40  GCAGC                GC^[ACGT]GC
KpnI          234  GGTACC               GGTAC^C
MaeI          175  CTAG                 C^TAG
MaeII         182  ACGT                 A^CGT
MaeIII        184  GTTAC                ^GT[ACGT]AC
MboI          130  GATC                 ^GATC
MvaI          231  CCTGG                CC^[AT]GG
MwoI          286  GCTCAGCATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         130  GATC                 ^GATC
NlaIII        292  CATG                 CATG^
NlaIV         234  GGTACC               GG[ACGT]^[ACGT]CC
NspI          291  GCATGC               [GA]CATG^[CT]
PshBI         108  ATTAAT               AT^TAAT
RsaI          235  GTAC                 GT^AC
SacI          256  GAGCTC               GAGCT^C
Sau3AI        130  GATC                 ^GATC
ScrFI         231  CCTGG                CC^[ACGT]GG
SfaNI          25  GATGC                GCATC(5/9)
SphI          291  GCATGC               GCATG^C
TseI           40  GCAGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AluI          102  AGCT                 AG^CT
              257  AGCT                
BanII         227  GAGCCC               G[GA]GC[CT]^C
              256  GAGCTC              
BmyI          227  GAGCCC               G[AGT]GC[ACT]^C
              256  GAGCTC              
Bsp1286I      227  GAGCCC               G[AGT]GC[ACT]^C
              256  GAGCTC              
Cac8I         291  GCATGC               GC[ACGT]^[ACGT]GC
              295  GCCAGC              
DdeI           97  CTGAG                C^T[ACGT]AG
              287  CTCAG               
MnlI           84  CCTC                 CCTC(7/6)
              154  GAGG                
TspRI         145  CACTG                CA[GC]TG[ACGT][ACGT]^
              243  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

MseI        Tru9I       Tsp509I