Restriction map for
pFN21AE5012
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII Acc65I AccI AccII AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AspI AviII BamHI BciVI BclI
BlpI Bpu1102I BsaBI BsiWI Bsp1407I
BspDI BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI Eco47III EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII KpnI
KspI MamI MfeI MluI MspA1I
MunI MvnI NaeI NdeI NgoMIV
NheI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PshAI PstI
PvuI PvuII RsrII SacII SalI
SbfI ScaI SexAI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AclI 1373 AACGTT AA^CGTT
AflII 1974 CTTAAG C^TTAAG
AhdI 310 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 824 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 310 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 2571 GGACC G^G[AT]CC
BalI 1188 TGGCCA TGG^CCA
BbrPI 1502 CACGTG CAC^GTG
BbvCI 1276 GCTGAGG CCTCAGC(-5/-2)
BcgI 2187 GCATTTCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1974 CTTAAG C^TTAAG
BsgI 1246 GTGCAG GTGCAG(16/14)
BsiEI 2365 CGGCCG CG[GA][CT]^CG
BsmBI 2025 GAGACG CGTCTC(1/5)
BsmI 2124 GCATTC GAATGC(1/-1)
BspHI 564 TCATGA T^CATGA
BspLU11I 1152 ACATGT A^CATGT
BsrBI 1016 CCGCTC CCGCTC(-3/-3)
BsrDI 1098 CATTGC GCAATG(2/0)
BstAPI 157 GCAAGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 791 TTCGAA TT^CGAA
BstEII 1513 GGTGACC G^GT[ACGT]ACC
BstPI 1513 GGTGACC G^GT[ACGT]ACC
EagI 2365 CGGCCG C^GGCCG
Eam1105I 310 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2365 CGGCCG C^GGCCG
Eco52I 2365 CGGCCG C^GGCCG
EcoO65I 1513 GGTGACC G^GT[ACGT]ACC
HgaI 282 GCGTC GACGC(5/10)
HpaI 2991 GTTAAC GTT^AAC
MluNI 1188 TGGCCA TGG^CCA
MscI 1188 TGGCCA TGG^CCA
NcoI 2835 CCATGG C^CATGG
NotI 2364 GCGGCCGC GC^GGCCGC
NspI 1152 ACATGT [GA]CATG^[CT]
NspV 791 TTCGAA TT^CGAA
PmaCI 1502 CACGTG CAC^GTG
PmlI 1502 CACGTG CAC^GTG
PpuMI 2570 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1373 AACGTT AA^CGTT
RcaI 564 TCATGA T^CATGA
SacI 208 GAGCTC GAGCT^C
SapI 368 GCTCTTC GCTCTTC(1/4)
SfiI 2966 GGCCAGTTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 791 TTCGAA TT^CGAA
SwaI 1341 ATTTAAAT ATTT^AAAT
XbaI 1075 TCTAGA T^CTAGA
XmnI 824 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 287 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
1192 CAGAAACTG
ApaI 2436 GGGCCC GGGCC^C
2974 GGGCCC
AseI 376 ATTAAT AT^TAAT
775 ATTAAT
AsnI 376 ATTAAT AT^TAAT
775 ATTAAT
BglI 1647 GCCTGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2967 GCCAGTTGGGC
BglII 745 AGATCT A^GATCT
2952 AGATCT
BsaAI 512 TACGTG [CT]AC^GT[GA]
1502 CACGTG
DraI 1342 TTTAAA TTT^AAA
2800 TTTAAA
DsaI 1916 CCACGG C^C[GA][CT]GG
2835 CCATGG
EcoNI 85 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1866 CCTTGAGGAGG
HincII 307 GTTGAC GT[CT]^[GA]AC
2991 GTTAAC
HindII 307 GTTGAC GT[CT]^[GA]AC
2991 GTTAAC
MslI 397 CATACTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
562 CATCATGATG
PflMI 286 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2671 CCAGAAGGTGG
PshBI 376 ATTAAT AT^TAAT
775 ATTAAT
TaqI 792 TCGA T^CGA
2866 TCGA
TthHB8I 792 TCGA T^CGA
2866 TCGA
Van91I 286 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2671 CCAGAAGGTGG
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccIII AciI AcsI AcyI AfaI
AflIII AluI AlwI AocI ApoI
AspHI AvaI AvrII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BlnI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MroI MseI
MspI MvaI MwoI NarI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII