##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DdeI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HindIII     HpaI        
HpaII       HphI        KasI        KpnI        KspI        
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AcsI          117  AAATTT               [GA]^AATT[CT]
ApoI          117  AAATTT               [GA]^AATT[CT]
AspHI         230  GAGCTC               G[AT]GC[AT]^C
AvaII          77  GGTCC                G^G[AT]CC
BanII         230  GAGCTC               G[GA]GC[CT]^C
BbvI           59  GCTGC                GCAGC(8/12)
BfaI           22  CTAG                 C^TAG
BmyI          230  GAGCTC               G[AGT]GC[ACT]^C
BsaI          218  GAGACC               GGTCTC(1/5)
BsaJI          93  CCCTGG               C^C[ACGT][ACGT]GG
BsiHKAI       230  GAGCTC               G[AT]GC[AT]^C
BsmAI         218  GAGAC                GTCTC(1/5)
BsmI          184  GAATGC               GAATGC(1/-1)
Bsp1286I      230  GAGCTC               G[AGT]GC[ACT]^C
BssKI          94  CCTGG                ^CC[ACGT]GG
BstBI         181  TTCGAA               TT^CGAA
BstNI          94  CCTGG                CC^[AT]GG
CfoI           90  GCGC                 GCG^C
Cfr13I         77  GGTCC                G^G[ACGT]CC
EarI          250  GAAGAG               CTCTTC(1/4)
EcoRII         94  CCTGG                ^CC[AT]GG
Fnu4HI         59  GCTGC                GC^[ACGT]GC
HaeIII         28  GGCC                 GG^CC
HhaI           90  GCGC                 GCG^C
HinP1I         90  GCGC                 G^CGC
HincII        158  GTCAAC               GT[CT]^[GA]AC
HindII        158  GTCAAC               GT[CT]^[GA]AC
HinfI         127  GATTC                G^A[ACGT]TC
ItaI           59  GCTGC                GC^[ACGT]GC
Ksp632I       250  GAAGAG               CTCTTC(1/4)
MaeI           22  CTAG                 C^TAG
MaeII         204  ACGT                 A^CGT
MvaI           94  CCTGG                CC^[AT]GG
NspV          181  TTCGAA               TT^CGAA
SacI          230  GAGCTC               GAGCT^C
Sau96I         77  GGTCC                G^G[ACGT]CC
ScrFI          94  CCTGG                CC^[ACGT]GG
SfaNI         109  GATGC                GCATC(5/9)
SfuI          181  TTCGAA               TT^CGAA
TaqI          182  TCGA                 T^CGA
TfiI          127  GATTC                G^A[AT]TC
TseI           59  GCTGC                G^C[AT]GC
TspRI          73  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       182  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

AluI           24  AGCT                 AG^CT
              231  AGCT                
BpmI           63  CTCCAG               CTGGAG(16/14)
               95  CTGGAG              
Cac8I          25  GCTGGC               GC[ACGT]^[ACGT]GC
               68  GCCAGC              
Tsp509I         9  AATT                 ^AATT
              118  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

BsrI        MboII       MnlI        MwoI