Restriction map for
pFN21AE1720
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaII AviII AvrII BalI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT22I FauI
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MspA1I
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AciI 664 GCGG CCGC(-3/-1)
AcyI 358 GGCGCC G[GA]^CG[CT]C
AluI 672 AGCT AG^CT
Asp700I 543 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 285 GGATCC G^GATCC
BanI 358 GGCGCC G^G[CT][GA]CC
BanII 643 GAGCCC G[GA]GC[CT]^C
BbeI 358 GGCGCC GGCGC^C
BfaI 299 CTAG C^TAG
BsaHI 358 GGCGCC G[GA]^CG[CT]C
BsaI 43 GGTCTC GGTCTC(1/5)
BsmBI 65 GAGACG CGTCTC(1/5)
BsrFI 423 ACCGGC [GA]^CCGG[CT]
BstYI 285 GGATCC [GA]^GATC[CT]
Cfr10I 423 ACCGGC [GA]^CCGG[CT]
DraIII 428 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 251 CCGTGG C^C[GA][CT]GG
EcoNI 492 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 349 GATATC GAT^ATC
EcoT14I 497 CCAAGG C^C[AT][AT]GG
HaeII 358 GGCGCC [GA]GCGC^[CT]
Hin1I 358 GGCGCC G[GA]^CG[CT]C
KasI 358 GGCGCC G^GCGCC
MaeI 299 CTAG C^TAG
MflI 285 GGATCC [GA]^GATC[CT]
MslI 48 CATGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 358 GGCGCC GG^CGCC
PaeR7I 512 CTCGAG C^TCGAG
PleI 604 GACTC GAGTC(4/5)
SfaNI 292 GATGC GCATC(5/9)
SfcI 198 CTACAG C^T[GA][CT]AG
SfoI 358 GGCGCC GGC^GCC
SmaI 243 CCCGGG CCC^GGG
SmlI 512 CTCGAG C^T[CT][GA]AG
StyI 497 CCAAGG C^C[AT][AT]GG
TaqI 513 TCGA T^CGA
TthHB8I 513 TCGA T^CGA
XhoI 512 CTCGAG C^TCGAG
XhoII 285 GGATCC [GA]^GATC[CT]
XmaCI 243 CCCGGG C^CCGGG
XmaI 243 CCCGGG C^CCGGG
XmnI 543 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 286 GATCC GGATC(4/5)
285 GGATC
AvaI 243 CCCGGG C^[CT]CG[GA]G
512 CTCGAG
BcnI 243 CCCGG CC^[GC]GG
244 CCGGG
BglI 91 GCCCATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
403 GCCTACCAGGC
BmyI 89 GTGCCC G[AGT]GC[ACT]^C
643 GAGCCC
BsoBI 243 CCCGGG C^[CT]CG[GA]G
512 CTCGAG
Bsp1286I 89 GTGCCC G[AGT]GC[ACT]^C
643 GAGCCC
BspHI 47 TCATGA T^CATGA
584 TCATGA
BstF5I 72 GGATG GGATG(2/0)
221 GGATG
CfoI 260 GCGC GCG^C
359 GCGC
DpnII 210 GATC ^GATC
286 GATC
DraII 98 AGGCCCT [GA]G^G[ACGT]CC[CT]
131 AGGGCCT
EcoO109I 98 AGGCCCT [GA]G^G[ACGT]CC[CT]
131 AGGGCCT
FokI 72 GGATG GGATG(9/13)
221 GGATG
HapII 244 CCGG C^CGG
424 CCGG
HhaI 260 GCGC GCG^C
359 GCGC
HinP1I 260 GCGC G^CGC
359 GCGC
HpaII 244 CCGG C^CGG
424 CCGG
MboI 210 GATC ^GATC
286 GATC
MspI 244 CCGG C^CGG
424 CCGG
NciI 243 CCCGG CC^[GC]GG
244 CCGGG
NdeII 210 GATC ^GATC
286 GATC
RcaI 47 TCATGA T^CATGA
584 TCATGA
Sau3AI 210 GATC ^GATC
286 GATC
TfiI 323 GATTC G^A[AT]TC
381 GATTC
Tsp45I 22 GTCAC ^GT[GC]AC
58 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
BbvI BpmI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstNI Cac8I
Cfr13I EcoRII Fnu4HI HaeIII HinfI
HphI ItaI MaeIII MboII MnlI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI TseI TspRI