Restriction map for
pFN21AE0612
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AhdI Alw44I AlwI
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MseI MunI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tru9I Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 835 GAATTT [GA]^AATT[CT]
AgeI 744 ACCGGT A^CCGGT
Aor51HI 869 AGCGCT AGC^GCT
ApoI 835 GAATTT [GA]^AATT[CT]
AspHI 358 GAGCTC G[AT]GC[AT]^C
AvaII 691 GGACC G^G[AT]CC
BbeI 788 GGCGCC GGCGC^C
BbvCI 730 GCTGAGG CCTCAGC(-5/-2)
BfaI 226 CTAG C^TAG
BglI 150 GCCCTACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 917 AGATCT A^GATCT
BsaI 590 GAGACC GGTCTC(1/5)
BsaWI 744 ACCGGT [AT]^CCGG[AT]
BsgI 907 GTGCAG GTGCAG(16/14)
BsiHKAI 358 GAGCTC G[AT]GC[AT]^C
BspMI 909 GCAGGT ACCTGC(4/8)
BsrBI 160 CCGCTC CCGCTC(-3/-3)
BsrI 724 ACTGG ACTGG(1/-1)
BssSI 163 CTCGTG CACGAG(-5/-1)
BstF5I 642 GGATG GGATG(2/0)
BstYI 917 AGATCT [GA]^GATC[CT]
Eco47III 869 AGCGCT AGC^GCT
FokI 642 GGATG GGATG(9/13)
HincII 1033 GTCAAC GT[CT]^[GA]AC
HindII 1033 GTCAAC GT[CT]^[GA]AC
KasI 788 GGCGCC G^GCGCC
MaeI 226 CTAG C^TAG
MflI 917 AGATCT [GA]^GATC[CT]
NarI 788 GGCGCC GG^CGCC
NheI 225 GCTAGC G^CTAGC
NspI 1029 GCATGT [GA]CATG^[CT]
PinAI 744 ACCGGT A^CCGGT
PleI 82 GACTC GAGTC(4/5)
PpuMI 690 GGGACCT [GA]G^G[AT]CC[CT]
PstI 665 CTGCAG CTGCA^G
SacI 358 GAGCTC GAGCT^C
SapI 682 GCTCTTC GCTCTTC(1/4)
SfcI 665 CTGCAG C^T[GA][CT]AG
SfoI 788 GGCGCC GGC^GCC
SgrAI 743 CACCGGTG C[GA]^CCGG[CT]G
SmlI 892 CTCAAG C^T[CT][GA]AG
TfiI 930 GAATC G^A[AT]TC
XhoII 917 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 425 GACGCC G[GA]^CG[CT]C
788 GGCGCC
AfaI 126 GTAC GT^AC
552 GTAC
AlwNI 175 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
764 CAGCTCCTG
BanI 741 GGCACC G^G[CT][GA]CC
788 GGCGCC
BpmI 574 CTGGAG CTGGAG(16/14)
725 CTGGAG
BsaHI 425 GACGCC G[GA]^CG[CT]C
788 GGCGCC
BseRI 565 GAGGAG GAGGAG(10/8)
733 GAGGAG
BsmAI 517 GAGAC GTCTC(1/5)
590 GAGAC
Bsp1407I 125 TGTACA T^GTACA
551 TGTACA
BsrDI 841 CATTGC GCAATG(2/0)
965 GCAATG
BsrGI 125 TGTACA T^GTACA
551 TGTACA
DpnII 879 GATC ^GATC
918 GATC
EarI 683 CTCTTC CTCTTC(1/4)
616 GAAGAG
HgaI 337 GACGC GACGC(5/10)
425 GACGC
Hin1I 425 GACGCC G[GA]^CG[CT]C
788 GGCGCC
HinfI 82 GACTC G^A[ACGT]TC
930 GAATC
HphI 631 GGTGA GGTGA(8/7)
747 GGTGA
Ksp632I 683 CTCTTC CTCTTC(1/4)
616 GAAGAG
MboI 879 GATC ^GATC
918 GATC
MboII 616 GAAGA GAAGA(8/7)
684 TCTTC
MslI 141 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1020 CATACCTGTG
NaeI 238 GCCGGC GCC^GGC
462 GCCGGC
NdeII 879 GATC ^GATC
918 GATC
NgoMIV 238 GCCGGC G^CCGGC
462 GCCGGC
PflMI 430 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1062 CCAACAAATGG
RsaI 126 GTAC GT^AC
552 GTAC
Sau3AI 879 GATC ^GATC
918 GATC
SfaNI 580 GCATC GCATC(5/9)
953 GCATC
SmaI 60 CCCGGG CCC^GGG
467 CCCGGG
SspBI 125 TGTACA T^GTACA
551 TGTACA
Tsp509I 824 AATT ^AATT
836 AATT
Van91I 430 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1062 CCAACAAATGG
XmaCI 60 CCCGGG C^CCGGG
467 CCCGGG
XmaI 60 CCCGGG C^CCGGG
467 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI ApaI AvaI
BanII BbvI BcnI BmyI BsaJI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeIII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI TaqI TseI
Tsp45I TspRI TthHB8I