Restriction map for
pFN21AE6053
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI AlwNI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BbvI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HgaI KpnI KspI
MaeI MamI MfeI MluI MroI
MspA1I MunI MvnI MwoI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TseI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AciI 696 CCGC CCGC(-3/-1)
AcyI 892 GGCGCC G[GA]^CG[CT]C
AflIII 206 ACATGT A^C[GA][CT]GT
Alw44I 417 GTGCAC G^TGCAC
AocI 870 CCTGAGG CC^T[ACGT]AGG
ApaI 505 GGGCCC GGGCC^C
ApaLI 417 GTGCAC G^TGCAC
BanI 892 GGCGCC G^G[CT][GA]CC
BanII 505 GGGCCC G[GA]GC[CT]^C
BbeI 892 GGCGCC GGCGC^C
BbsI 94 GTCTTC GAAGAC(2/6)
BpuAI 94 GTCTTC GAAGAC(2/6)
BsaHI 892 GGCGCC G[GA]^CG[CT]C
BsaI 642 GGTCTC GGTCTC(1/5)
BsmAI 643 GTCTC GTCTC(1/5)
BsmI 876 GAATGC GAATGC(1/-1)
BspHI 401 TCATGA T^CATGA
BspLU11I 206 ACATGT A^CATGT
BspMI 715 ACCTGC ACCTGC(4/8)
BstXI 233 CCAAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 677 AGATCC [GA]^GATC[CT]
Bsu36I 870 CCTGAGG CC^T[ACGT]AGG
Cac8I 555 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 893 GCGC GCG^C
DraII 505 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 761 CCATGG C^C[GA][CT]GG
Eco81I 870 CCTGAGG CC^T[ACGT]AGG
EcoNI 353 CCTATGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 505 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 761 CCATGG C^C[AT][AT]GG
EcoT22I 285 ATGCAT ATGCA^T
Fnu4HI 695 GCCGC GC^[ACGT]GC
HaeII 892 GGCGCC [GA]GCGC^[CT]
HapII 510 CCGG C^CGG
HhaI 893 GCGC GCG^C
Hin1I 892 GGCGCC G[GA]^CG[CT]C
HinP1I 893 GCGC G^CGC
HincII 577 GTTAAC GT[CT]^[GA]AC
HindII 577 GTTAAC GT[CT]^[GA]AC
HindIII 157 AAGCTT A^AGCTT
HpaI 577 GTTAAC GTT^AAC
HpaII 510 CCGG C^CGG
ItaI 695 GCCGC GC^[ACGT]GC
KasI 892 GGCGCC G^GCGCC
MaeII 436 ACGT A^CGT
MaeIII 322 GTTAC ^GT[ACGT]AC
MflI 677 AGATCC [GA]^GATC[CT]
MspI 510 CCGG C^CGG
NarI 892 GGCGCC GG^CGCC
NcoI 761 CCATGG C^CATGG
NsiI 285 ATGCAT ATGCA^T
RcaI 401 TCATGA T^CATGA
SfcI 864 CTACAG C^T[GA][CT]AG
SfoI 892 GGCGCC GGC^GCC
SmaI 509 CCCGGG CCC^GGG
SmlI 550 CTCAAG C^T[CT][GA]AG
StuI 704 AGGCCT AGG^CCT
StyI 761 CCATGG C^C[AT][AT]GG
XhoII 677 AGATCC [GA]^GATC[CT]
XmaCI 509 CCCGGG C^CCGGG
XmaI 509 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 476 AAATTC [GA]^AATT[CT]
536 AAATTC
ApoI 476 AAATTC [GA]^AATT[CT]
536 AAATTC
AspHI 417 GTGCAC G[AT]GC[AT]^C
905 GAGCAC
AvaI 509 CCCGGG C^[CT]CG[GA]G
661 CTCGGG
BalI 194 TGGCCA TGG^CCA
368 TGGCCA
BcnI 509 CCCGG CC^[GC]GG
510 CCGGG
BpmI 910 CTCCAG CTGGAG(16/14)
408 CTGGAG
BsiHKAI 417 GTGCAC G[AT]GC[AT]^C
905 GAGCAC
BsoBI 509 CCCGGG C^[CT]CG[GA]G
661 CTCGGG
BsrI 63 ACTGG ACTGG(1/-1)
387 CCAGT
EaeI 194 TGGCCA [CT]^GGCC[GA]
368 TGGCCA
MluNI 194 TGGCCA TGG^CCA
368 TGGCCA
MscI 194 TGGCCA TGG^CCA
368 TGGCCA
MseI 578 TTAA T^TAA
839 TTAA
NciI 509 CCCGG CC^[GC]GG
510 CCGGG
NspI 206 ACATGT [GA]CATG^[CT]
650 ACATGC
Tru9I 578 TTAA T^TAA
839 TTAA
Tsp509I 477 AATT ^AATT
537 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AfaI AluI AlwI AvaII BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cfr13I
DdeI DpnII EarI EcoRII FokI
HaeIII HinfI HphI Ksp632I MboI
MboII MnlI MslI MvaI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI TfiI TspRI