Restriction map for
pFN21AE5471
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfaI BglI BglII BlnI BmyI
BsaAI BsaBI BsaHI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MaeI
MamI MluI MluNI MscI MspA1I
MvnI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TfiI Tth111I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 73 TCCGGA T^CCGGA
AflII 459 CTTAAG C^TTAAG
AvaI 480 CTCGAG C^[CT]CG[GA]G
BclI 617 TGATCA T^GATCA
BfrI 459 CTTAAG C^TTAAG
BlpI 186 GCTGAGC GC^T[ACGT]AGC
BpmI 226 CTGGAG CTGGAG(16/14)
Bpu1102I 186 GCTGAGC GC^T[ACGT]AGC
BsaWI 73 TCCGGA [AT]^CCGG[AT]
BseAI 73 TCCGGA T^CCGGA
BseRI 528 CTCCTC GAGGAG(10/8)
BsmFI 704 GGGAC GGGAC(10/14)
BsoBI 480 CTCGAG C^[CT]CG[GA]G
BspEI 73 TCCGGA T^CCGGA
BsrDI 686 GCAATG GCAATG(2/0)
BsrFI 868 GCCGGC [GA]^CCGG[CT]
BstYI 602 GGATCT [GA]^GATC[CT]
Cac8I 868 GCCGGC GC[ACGT]^[ACGT]GC
CelII 186 GCTGAGC GC^T[ACGT]AGC
Cfr10I 868 GCCGGC [GA]^CCGG[CT]
DraII 704 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 539 CCACGG C^C[GA][CT]GG
EcoO109I 704 GGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 617 TGATCA T^GATCA
HincII 574 GTCAAC GT[CT]^[GA]AC
HindII 574 GTCAAC GT[CT]^[GA]AC
HindIII 462 AAGCTT A^AGCTT
HinfI 963 GACTC G^A[ACGT]TC
MaeII 799 ACGT A^CGT
MfeI 779 CAATTG C^AATTG
MflI 602 GGATCT [GA]^GATC[CT]
MroI 73 TCCGGA T^CCGGA
MseI 460 TTAA T^TAA
MslI 60 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 779 CAATTG C^AATTG
NaeI 868 GCCGGC GCC^GGC
NgoMIV 868 GCCGGC G^CCGGC
PaeR7I 480 CTCGAG C^TCGAG
PflMI 320 CCAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 963 GACTC GAGTC(4/5)
PpuMI 704 GGGACCC [GA]G^G[AT]CC[CT]
SapI 911 GAAGAGC GCTCTTC(1/4)
ScaI 551 AGTACT AGT^ACT
SexAI 578 ACCTGGT A^CC[AT]GGT
SfcI 208 CTACAG C^T[GA][CT]AG
SspI 832 AATATT AAT^ATT
TaqI 481 TCGA T^CGA
Tru9I 460 TTAA T^TAA
TthHB8I 481 TCGA T^CGA
Van91I 320 CCAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 480 CTCGAG C^TCGAG
XhoII 602 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 171 CCGC CCGC(-3/-1)
924 GCGG
AfaI 552 GTAC GT^AC
880 GTAC
AlwI 648 GATCC GGATC(4/5)
602 GGATC
AlwNI 822 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
961 CAGACTCTG
Asp700I 231 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
549 GAAGTACTTC
AvaII 698 GGTCC G^G[AT]CC
705 GGACC
BbsI 349 GTCTTC GAAGAC(2/6)
490 GTCTTC
BpuAI 349 GTCTTC GAAGAC(2/6)
490 GTCTTC
BsaI 562 GAGACC GGTCTC(1/5)
69 GGTCTC
BsrI 91 ACTGG ACTGG(1/-1)
163 ACTGG
BstEII 51 GGTCACC G^GT[ACGT]ACC
166 GGTGACC
BstPI 51 GGTCACC G^GT[ACGT]ACC
166 GGTGACC
EaeI 119 CGGCCA [CT]^GGCC[GA]
870 CGGCCA
EcoO65I 51 GGTCACC G^GT[ACGT]ACC
166 GGTGACC
EcoT14I 99 CCTTGG C^C[AT][AT]GG
427 CCTTGG
HapII 74 CCGG C^CGG
869 CCGG
HpaII 74 CCGG C^CGG
869 CCGG
MaeIII 52 GTCAC ^GT[ACGT]AC
167 GTGAC
MspI 74 CCGG C^CGG
869 CCGG
RsaI 552 GTAC GT^AC
880 GTAC
StyI 99 CCTTGG C^C[AT][AT]GG
427 CCTTGG
Tsp45I 52 GTCAC ^GT[GC]AC
167 GTGAC
XmnI 231 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
549 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BsmAI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HphI ItaI Ksp632I
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SmlI TseI Tsp509I
TspRI