Restriction map for
pFN21AE6014
##################################
# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspLU11I
BsrBI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaI
KasI KpnI KspI MamI MluI
MluNI MscI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 875 TCCGGA T^CCGGA
AcsI 28 GAATTT [GA]^AATT[CT]
ApoI 28 GAATTT [GA]^AATT[CT]
AseI 111 ATTAAT AT^TAAT
AsnI 111 ATTAAT AT^TAAT
Asp700I 705 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 360 GGACC G^G[AT]CC
BfaI 17 CTAG C^TAG
BpmI 202 CTGGAG CTGGAG(16/14)
BsaAI 775 TACGTG [CT]AC^GT[GA]
BsaWI 875 TCCGGA [AT]^CCGG[AT]
BseAI 875 TCCGGA T^CCGGA
BsmAI 646 GTCTC GTCTC(1/5)
BspEI 875 TCCGGA T^CCGGA
BspHI 134 TCATGA T^CATGA
BspMI 418 ACCTGC ACCTGC(4/8)
BsrDI 349 GCAATG GCAATG(2/0)
BsrFI 474 GCCGGT [GA]^CCGG[CT]
Cac8I 795 GCAGGC GC[ACGT]^[ACGT]GC
Cfr10I 474 GCCGGT [GA]^CCGG[CT]
DdeI 388 CTGAG C^T[ACGT]AG
DraII 896 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 896 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 39 CCTTGG C^C[AT][AT]GG
HincII 926 GTCAAC GT[CT]^[GA]AC
HindII 926 GTCAAC GT[CT]^[GA]AC
MaeI 17 CTAG C^TAG
MroI 875 TCCGGA T^CCGGA
PshBI 111 ATTAAT AT^TAAT
PstI 160 CTGCAG CTGCA^G
RcaI 134 TCATGA T^CATGA
SapI 300 GCTCTTC GCTCTTC(1/4)
SfcI 160 CTGCAG C^T[GA][CT]AG
SmlI 676 CTCAAG C^T[CT][GA]AG
StyI 39 CCTTGG C^C[AT][AT]GG
TspRI 24 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 705 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 309 GTAC GT^AC
393 GTAC
BstYI 206 AGATCC [GA]^GATC[CT]
680 AGATCC
Cfr13I 360 GGACC G^G[ACGT]CC
897 GGCCC
EarI 301 CTCTTC CTCTTC(1/4)
595 CTCTTC
Ksp632I 301 CTCTTC CTCTTC(1/4)
595 CTCTTC
MaeII 156 ACGT A^CGT
776 ACGT
MflI 206 AGATCC [GA]^GATC[CT]
680 AGATCC
MseI 112 TTAA T^TAA
601 TTAA
MslI 129 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
399 CATCATTATG
MspA1I 759 CAGCTG C[AC]G^C[GT]G
800 CAGCTG
MwoI 44 GCTTCCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
752 GCTACGGCAGC
PvuII 759 CAGCTG CAG^CTG
800 CAGCTG
RsaI 309 GTAC GT^AC
393 GTAC
Sau96I 360 GGACC G^G[ACGT]CC
897 GGCCC
SfaNI 237 GATGC GCATC(5/9)
264 GATGC
Tru9I 112 TTAA T^TAA
601 TTAA
XhoII 206 AGATCC [GA]^GATC[CT]
680 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsiYI
BslI BsmFI BssKI BstF5I BstNI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeIII MboI MboII MfeI MnlI
MspI MunI MvaI NdeII NlaIII
Sau3AI ScrFI TseI Tsp45I Tsp509I