Restriction map for
pFN21AE3955
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI AspI
AviII AvrII BamHI BbeI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsiWI BsmBI BsmI
BspDI BspHI BspLU11I BsrBI BsrDI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DrdI Eco47III
EcoRI EcoRV EcoT22I FbaI FspI
HindIII HpaI KasI KspI MamI
MfeI MluI MseI MunI NarI
NdeI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tru9I Tsp45I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 2256 TCCGGA T^CCGGA
AcyI 781 GGCGTC G[GA]^CG[CT]C
AhdI 1510 GACAAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1639 CCTGAGG CC^T[ACGT]AGG
AspEI 1510 GACAAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1057 GAGCTC G[AT]GC[AT]^C
BbsI 1200 GAAGAC GAAGAC(2/6)
BbvCI 775 GCTGAGG CCTCAGC(-5/-2)
BcgI 852 CGAAGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1200 GAAGAC GAAGAC(2/6)
BsaHI 781 GGCGTC G[GA]^CG[CT]C
BsaI 275 GAGACC GGTCTC(1/5)
BsaWI 2256 TCCGGA [AT]^CCGG[AT]
BseAI 2256 TCCGGA T^CCGGA
BsiEI 153 CGGCCG CG[GA][CT]^CG
BsiHKAI 1057 GAGCTC G[AT]GC[AT]^C
Bsp1407I 1838 TGTACA T^GTACA
BspEI 2256 TCCGGA T^CCGGA
BsrGI 1838 TGTACA T^GTACA
BssSI 1330 CACGAG CACGAG(-5/-1)
BstEII 2178 GGTTACC G^GT[ACGT]ACC
BstPI 2178 GGTTACC G^GT[ACGT]ACC
Bsu36I 1639 CCTGAGG CC^T[ACGT]AGG
EagI 153 CGGCCG C^GGCCG
Eam1105I 1510 GACAAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1285 GAAGAG CTCTTC(1/4)
EclXI 153 CGGCCG C^GGCCG
Eco52I 153 CGGCCG C^GGCCG
Eco81I 1639 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2178 GGTTACC G^GT[ACGT]ACC
FseI 341 GGCCGGCC GGCCGG^CC
Hin1I 781 GGCGTC G[GA]^CG[CT]C
HincII 2095 GTCAAC GT[CT]^[GA]AC
HindII 2095 GTCAAC GT[CT]^[GA]AC
Ksp632I 1285 GAAGAG CTCTTC(1/4)
MroI 2256 TCCGGA T^CCGGA
NaeI 342 GCCGGC GCC^GGC
NcoI 2186 CCATGG C^CATGG
NgoMIV 342 GCCGGC G^CCGGC
NotI 152 GCGGCCGC GC^GGCCGC
SacI 1057 GAGCTC GAGCT^C
SfaNI 1500 GATGC GCATC(5/9)
SfiI 792 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 1838 TGTACA T^GTACA
TaqI 2088 TCGA T^CGA
TfiI 1440 GAATC G^A[AT]TC
Tsp509I 1407 AATT ^AATT
TthHB8I 2088 TCGA T^CGA
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1153 GGTACC G^GTACC
2201 GGTACC
AlwNI 185 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
556 CAGTTGCTG
Asp700I 1380 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1593 GAAACGCTTC
Asp718I 1153 GGTACC G^GTACC
2201 GGTACC
BalI 902 TGGCCA TGG^CCA
1859 TGGCCA
BbrPI 838 CACGTG CAC^GTG
1828 CACGTG
BsaAI 838 CACGTG [CT]AC^GT[GA]
1828 CACGTG
BsrI 1955 ACTGG ACTGG(1/-1)
501 CCAGT
BstAPI 566 GCAGAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
810 GCAGGAGGTGC
BstYI 959 AGATCC [GA]^GATC[CT]
1559 AGATCC
DraIII 430 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1774 CACGCGGTG
HgaI 158 GCGTC GACGC(5/10)
782 GCGTC
KpnI 1153 GGTACC GGTAC^C
2201 GGTACC
MaeII 839 ACGT A^CGT
1829 ACGT
MaeIII 2179 GTTAC ^GT[ACGT]AC
2222 GTAAC
MflI 959 AGATCC [GA]^GATC[CT]
1559 AGATCC
MluNI 902 TGGCCA TGG^CCA
1859 TGGCCA
MscI 902 TGGCCA TGG^CCA
1859 TGGCCA
NheI 720 GCTAGC G^CTAGC
1696 GCTAGC
NlaIII 2029 CATG CATG^
2187 CATG
PleI 756 GACTC GAGTC(4/5)
1220 GAGTC
PmaCI 838 CACGTG CAC^GTG
1828 CACGTG
PmlI 838 CACGTG CAC^GTG
1828 CACGTG
SmlI 883 CTTGAG C^T[CT][GA]AG
1399 CTCAAG
StuI 386 AGGCCT AGG^CCT
2022 AGGCCT
XhoII 959 AGATCC [GA]^GATC[CT]
1559 AGATCC
XmnI 1380 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1593 GAAACGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIV
PflMI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfcI
SmaI StyI TseI TspRI Van91I
XcmI XmaCI XmaI