Restriction map for
pFN21AE2415
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BbeI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmFI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI Tsp45I
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 1347 CCGC CCGC(-3/-1)
AcsI 39 GAATTC [GA]^AATT[CT]
AflIII 1259 ACGTGT A^C[GA][CT]GT
ApoI 39 GAATTC [GA]^AATT[CT]
AspHI 434 GAGCAC G[AT]GC[AT]^C
BamHI 1343 GGATCC G^GATCC
BanI 372 GGTGCC G^G[CT][GA]CC
BbrPI 385 CACGTG CAC^GTG
BclI 1295 TGATCA T^GATCA
BseRI 1017 CTCCTC GAGGAG(10/8)
BsgI 182 GTGCAG GTGCAG(16/14)
BsiHKAI 434 GAGCAC G[AT]GC[AT]^C
BsmBI 662 GAGACG CGTCTC(1/5)
Bsp1407I 1180 TGTACA T^GTACA
BspMI 453 GCAGGT ACCTGC(4/8)
BsrDI 1112 GCAATG GCAATG(2/0)
BsrGI 1180 TGTACA T^GTACA
BstAPI 436 GCACTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1343 GGATCC [GA]^GATC[CT]
Cac8I 762 GCTTGC GC[ACGT]^[ACGT]GC
EarI 1209 GAAGAG CTCTTC(1/4)
EcoRI 39 GAATTC G^AATTC
FbaI 1295 TGATCA T^GATCA
HincII 1120 GTCAAC GT[CT]^[GA]AC
HindII 1120 GTCAAC GT[CT]^[GA]AC
Ksp632I 1209 GAAGAG CTCTTC(1/4)
MaeIII 976 GTTAC ^GT[ACGT]AC
MflI 1343 GGATCC [GA]^GATC[CT]
MwoI 436 GCACTGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 1040 CATATG CA^TATG
PflMI 1188 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 385 CACGTG CAC^GTG
PmlI 385 CACGTG CAC^GTG
SspBI 1180 TGTACA T^GTACA
SspI 297 AATATT AAT^ATT
Van91I 1188 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1343 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 304 GTAGAC GT^[AC][GT]AC
1324 GTAGAC
Asp700I 1088 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1209 GAAGAGATTC
BanII 380 GAGCCC G[GA]GC[CT]^C
419 GGGCTC
BbsI 159 GAAGAC GAAGAC(2/6)
634 GTCTTC
BbvCI 1019 CCTCAGC CCTCAGC(-5/-2)
1218 CCTCAGC
BpuAI 159 GAAGAC GAAGAC(2/6)
634 GTCTTC
BsaAI 385 CACGTG [CT]AC^GT[GA]
1258 TACGTG
BsmAI 58 GAGAC GTCTC(1/5)
662 GAGAC
BstF5I 441 GGATG GGATG(2/0)
857 GGATG
FokI 441 GGATG GGATG(9/13)
857 GGATG
HphI 82 GGTGA GGTGA(8/7)
1293 GGTGA
MslI 921 CACACTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1191 CACCCTGGTG
PstI 580 CTGCAG CTGCA^G
1250 CTGCAG
SfcI 580 CTGCAG C^T[GA][CT]AG
1250 CTGCAG
SmlI 192 CTTGAG C^T[CT][GA]AG
991 CTCAAG
XmnI 1088 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1209 GAAGAGATTC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AfaI AluI AlwI BbvI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstNI DdeI DpnII
EcoRII Fnu4HI HinfI ItaI MaeII
MboI MboII MnlI MseI MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I