##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MspA1I      MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI          532  GTCTAC               GT^[AC][GT]AC
ApaI           38  GGGCCC               GGGCC^C
AvaI          439  CCCGGG               C^[CT]CG[GA]G
BanII          38  GGGCCC               G[GA]GC[CT]^C
BbsI          279  GTCTTC               GAAGAC(2/6)
BpuAI         279  GTCTTC               GAAGAC(2/6)
BsmAI         271  GTCTC                GTCTC(1/5)
BsmBI         270  CGTCTC               CGTCTC(1/5)
BsoBI         439  CCCGGG               C^[CT]CG[GA]G
BssSI          99  CTCGTG               CACGAG(-5/-1)
BstXI         149  CCATCGGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          208  GCGC                 GCG^C
DpnII         220  GATC                 ^GATC
DraIII        376  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           79  CCGTGG               C^C[GA][CT]GG
EcoRV         252  GATATC               GAT^ATC
HaeII         207  GGCGCT               [GA]GCGC^[CT]
HgaI          390  GACGC                GACGC(5/10)
HhaI          208  GCGC                 GCG^C
HinP1I        208  GCGC                 G^CGC
MaeII         269  ACGT                 A^CGT
MaeIII         10  GTCAC                ^GT[ACGT]AC
MboI          220  GATC                 ^GATC
MboII         280  TCTTC                GAAGA(8/7)
NdeII         220  GATC                 ^GATC
NspI          446  ACATGC               [GA]CATG^[CT]
PpuMI         656  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI          632  CTGCAG               CTGCA^G
Sau3AI        220  GATC                 ^GATC
SfaNI         563  GCATC                GCATC(5/9)
SfcI          632  CTGCAG               C^T[GA][CT]AG
SmaI          439  CCCGGG               CCC^GGG
Tsp45I         10  GTCAC                ^GT[GC]AC
XmaCI         439  CCCGGG               C^CCGGG
XmaI          439  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AfaI          267  GTAC                 GT^AC
              465  GTAC                
AvaII         508  GGTCC                G^G[AT]CC
              657  GGTCC               
BpmI          525  CTCCAG               CTGGAG(16/14)
              651  CTCCAG              
BsgI          432  CTGCAC               GTGCAG(16/14)
              493  GTGCAG              
BsrI          401  CCAGT                ACTGG(1/-1)
              527  CCAGT               
BstF5I        564  CATCC                GGATG(2/0)
               29  GGATG               
EcoT14I       614  CCTTGG               C^C[AT][AT]GG
              641  CCAAGG              
FokI          564  CATCC                GGATG(9/13)
               29  GGATG               
HinfI           8  GAGTC                G^A[ACGT]TC
              646  GAGTC               
PleI            8  GAGTC                GAGTC(4/5)
              646  GAGTC               
RsaI          267  GTAC                 GT^AC
              465  GTAC                
StyI          614  CCTTGG               C^C[AT][AT]GG
              641  CCAAGG              
TaqI          188  TCGA                 T^CGA
              218  TCGA                
TspRI         402  CAGTG                CA[GC]TG[ACGT][ACGT]^
              528  CAGTG               
TthHB8I       188  TCGA                 T^CGA
              218  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AluI        AlwNI       BanI        BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BstNI       
Cac8I       Cfr13I      DdeI        DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HpaII       HphI        ItaI        MnlI        MslI        
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       Sau96I      ScrFI       TseI